Firstly, the testis organ of the rats was removed to exclude the influence of intrinsic testosterone except for the rats in the control group. The spermatic cord and blood vessels were ligated with Silkam sutures 3/8–16 mm (B. Braun Surgical SA, Rubi, Spain) and the testis organ was resected. In order to induce BPH in rats, a subcutaneous injection of testosterone (20 mg/kg; Wako chemicals, Tokyo, Japan) for 4 weeks after castration was carried out. And, next day, rats were divided into 4 groups (each group, n = 10): (1) control group, (2) testosterone-induced (subcutaneous) BPH group, and (3) KH053 treated group (200 mg/kg oral administration). (4) Finasteride (Sigma–Aldrich, St. Louis, MO, USA) treat group as positive group. Based on a previous study, we treated rats with 200 mg/kg of KH053 (Hong et al., 2013 (link), Kim et al., 2014 (link)). All materials were administered to the animals in the morning for 30 days.
Testosterone
Manufactured by Fujifilm
Sourced in Japan
Testosterone is a laboratory equipment product manufactured by Fujifilm. It is used to measure and analyze the levels of the testosterone hormone in biological samples. The product's core function is to provide accurate and reliable results for testosterone quantification, which is essential for various medical and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Silkam sutures, by B. Braun (2 mentions)
Dimethyl sulfoxide (dmso), by Fujifilm (2 mentions)
Finasteride, by Merck Group (1 mentions)
Zoletil 50, by Virbac (1 mentions)
Hydrocortisone, by Fujifilm (1 mentions)
Tebufenozide, by Fujifilm (1 mentions)
Corticosterone, by Fujifilm (1 mentions)
Ecdysone, by Merck Group (1 mentions)
O nitrophenyl β d galactopyranoside, by Merck Group (1 mentions)
20 hydroxyecdysone 20e, by Merck Group (1 mentions)
Restriction enzymes, by Fujifilm (1 mentions)
Hydrogen peroxide, by Fujifilm (1 mentions)
Urea, by Fujifilm (1 mentions)
Ultrapure water, by Thermo Fisher Scientific (1 mentions)
Sodium chloride (nacl), by Fujifilm (1 mentions)
Disodium hydrogen phosphate, by Fujifilm (1 mentions)
Progesterone, by Fujifilm (1 mentions)
Sodium nitrate, by Fujifilm (1 mentions)
Mucin, by Fujifilm (1 mentions)
Glucose, by Fujifilm (1 mentions)
9 protocols using testosterone
1
Rat Model for Benign Prostatic Hyperplasia
2
Rat Model of Benign Prostatic Hyperplasia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The BPH animal model was made following a previously described method [9 (link)]. The rats were anesthetized with Zoletil 50® (50 mg/kg; Virbac Laboratories, Carros, France). The testes and epididymis were exposed through a midline ventral scrotal incision, and the extratesticular rete testis together with the pampiniform plexus vessels were ligated. Then, the testes were removed, and the ligated efferent ductules and epididymis were returned to the scrotum. In the sham-operation rats, the testes were exposed, manipulated, and then reinserted into the scrotum. After surgery, the scrotal incision was closed by suturing. After orchiectomy, BPH was induced by a subcutaneous injection of testosterone (20 mg/kg; Wako Pure Chemical Industries, Ltd., Osaka, Japan) for 30 days.
3
Synthesis and Characterization of Tetrahydroquinoline Compound
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A tetrahydroquinoline (THQ)‐type compound (C23H19BrF2N2O) was synthesized as previously described 40 , 41 . Dimethyl sulfoxide (DMSO), tebufenozide, methoxyfenozide, chromafenozide, corticosterone, hydrocortisone, and testosterone were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Dithiothreitol (DTT), 17β‐estradiol (E2), and progesterone were obtained from Nacalai Tesque (Kyoto, Japan). 20‐Hydroxyecdysone (20E), ecdysone, ponasterone A (Pon A), aldosterone, halofenozide, and o‐nitrophenyl‐β‐d ‐galactopyranoside (ONPG) were purchased from Sigma Aldrich Chemical Co. (St. Louis, MO, USA). Restriction enzymes, DNA modification enzymes, and other chemicals were obtained from Wako Pure Chemical Industries, Ltd., TaKaRa Bio Inc. (Otsu, Japan), or TOYOBO Co., Ltd. (Osaka, Japan).
4
Electrochemical Detection of Biomolecules
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cortisol (C21H30O5, hydrocortisone), β-CD [(C6H10O5)7], potassium HCF(II)
trihydrate (K4[Fe(CN)6]·3H2O),
potassium HCF(III) (K4[Fe(CN)6]), graphite powder,
sulfuric acid (H2SO4, 98%), sodium nitrate (NaNO3), potassium permanganate (KMnO4), hydrogen peroxide
(H2O2, 30% in water), 1 M hydrochloric acid
(HCl), ethanol, dimethyl sulfoxide (DMSO), glucose, urea, sodiuml -lactate, cholesterol, progesterone, testosterone, sodium chloride
(NaCl), disodium hydrogen phosphate (Na2HPO4), anhydrous calcium chloride (CaCl2), and mucin were
procured from Fujifilm WAKO (Japan). Phosphate buffer saline (PBS,
pH 7.4) and ultrapure water were obtained from Gibco (Thermofischer).
Pyrrole (C4H5N) was obtained from Tokyo Chemicals
(TCI, Japan). All chemicals were used as received. Rod-type glassy
carbon electrodes (GCE, surface area = 0.07 cm2) were obtained
from ALS Co., Ltd. (Japan).
trihydrate (K4[Fe(CN)6]·3H2O),
potassium HCF(III) (K4[Fe(CN)6]), graphite powder,
sulfuric acid (H2SO4, 98%), sodium nitrate (NaNO3), potassium permanganate (KMnO4), hydrogen peroxide
(H2O2, 30% in water), 1 M hydrochloric acid
(HCl), ethanol, dimethyl sulfoxide (DMSO), glucose, urea, sodium
(NaCl), disodium hydrogen phosphate (Na2HPO4), anhydrous calcium chloride (CaCl2), and mucin were
procured from Fujifilm WAKO (Japan). Phosphate buffer saline (PBS,
pH 7.4) and ultrapure water were obtained from Gibco (Thermofischer).
Pyrrole (C4H5N) was obtained from Tokyo Chemicals
(TCI, Japan). All chemicals were used as received. Rod-type glassy
carbon electrodes (GCE, surface area = 0.07 cm2) were obtained
from ALS Co., Ltd. (Japan).
5
Testosterone and Antiandrogen Receptor Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Testosterone and flutamide (an androgen receptor inhibitor) were purchased from Wako Pure Chemical Industries (Osaka, Japan). Aromatase Inhibitor I was purchased from Merck Millipore (Billerica, MA, USA). LY 294002 was purchased from Sigma-Aldrich (St. Louis, MO, USA). All rabbit monoclonal anti-human Akt antibodies, monoclonal anti-human phospho-Akt antibodies, rabbit monoclonal anti-human FoxO3a antibodies, monoclonal anti-human phospho-FoxO3a antibodies, rabbit monoclonal anti-human beta actin antibodies and rabbit polyclonal anti-human Bim antibodies used for immunoblotting and immunohistochemistry were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Rabbit monoclonal anti-human FSH receptor antibodies were purchased from Abcam (Cambridge, MA, USA).
6
Rat Model of Benign Prostatic Hyperplasia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The testes of the rats in the BPH and the P. ginseng groups were removed to exclude the influence of intrinsic testosterone. The spermatic cord and blood vessels were ligated with Silkam sutures 3/8-16 mm (B.Braun Surgical SA, Rubi, Spain) and resected. BPH was induced by subcutaneous injection of testosterone (20 mg/kg; Wako chemicals, Tokyo, Japan) for 4 weeks after castration. Rats were divided into 3 groups (each group, n=10): (1) control group, (2) testosterone-induced (subcutaneous) BPH group, and (3) P. ginseng treated group (200 mg/kg oral administration; Sigma-Aldrich, St. Louis, MO, USA). Based on previous studies, we treated rats with 200 mg/kg of P. ginseng [20 (link),21 (link)]. All materials were administered to the animals once daily for 4 weeks, and body weight was measured weekly. After 4 weeks, all animals were fasted overnight. Blood was collected in ethylenediaminetetraacetic acid tubes, placed on ice, and the serum was immediately separated and stored at -20℃. After the animals were sacrificed, the tissue of prostate was stored in formaldehyde solution for light microscopic observation. The rest of the prostate was stored at -70℃ for subsequent analysis.
7
Procurement of Diverse Chemical Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Abietic acid, ADD, DHEA, methyltestosterone,
phenanthrene, and progesterone were purchased from Tokyo Chemical
Industry (Tokyo, Japan). DehydroAbietic acid, dibenzothiophene, 7-ethoxycoumarin,
ibuprofen, and testosterone were purchased from Fujifilm Wako Pure
Chemical (Osaka, Japan). Pyrene was purchased from Sigma-Aldrich Japan
(Tokyo, Japan). Diclofenac was purchased from Combi-Blocks USA (San
Diego, CA, USA). Ferruginol was kindly provided by Dr. H. Suhara (Miyazaki
Prefectural Wood Utilization Research Center, Japan). Yeast nitrogen
base without amino acids was purchased from Formedium (Hunstanton,
UK). Dropout supplements (DOS) were purchased from TaKaRa Bio USA
(Mountain View, CA, USA). Custom-synthesized oligonucleotide primers
were obtained from Sigma-Aldrich Japan. All other chemicals were of
reagent grade. Deionized water was obtained using a Barnstead Smart2Pure
System (Thermo Fisher Scientific, Waltham, MA, USA).
phenanthrene, and progesterone were purchased from Tokyo Chemical
Industry (Tokyo, Japan). DehydroAbietic acid, dibenzothiophene, 7-ethoxycoumarin,
ibuprofen, and testosterone were purchased from Fujifilm Wako Pure
Chemical (Osaka, Japan). Pyrene was purchased from Sigma-Aldrich Japan
(Tokyo, Japan). Diclofenac was purchased from Combi-Blocks USA (San
Diego, CA, USA). Ferruginol was kindly provided by Dr. H. Suhara (Miyazaki
Prefectural Wood Utilization Research Center, Japan). Yeast nitrogen
base without amino acids was purchased from Formedium (Hunstanton,
UK). Dropout supplements (DOS) were purchased from TaKaRa Bio USA
(Mountain View, CA, USA). Custom-synthesized oligonucleotide primers
were obtained from Sigma-Aldrich Japan. All other chemicals were of
reagent grade. Deionized water was obtained using a Barnstead Smart2Pure
System (Thermo Fisher Scientific, Waltham, MA, USA).
8
Akr1A1 Mice, Gonadectomy, Sex Hormones
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The male and female Akr1A1eGFP/+ mice were divided into 4 groups. Groups 1, 2 and 3 underwent gonadectomy during early puberty (4 weeks) and received subcutaneous (S.C.) injections of testosterone (2 mg/kg; Wako Pure Chemical Industries, Japan), 17β-estradiol (50 μg/kg; Wako Pure Chemical Industries) in corn oil or corn oil only, respectively, once a day for 2 weeks. Group 4 consisted of the mature intact female mice (8 weeks old) that received S.C. injections of testosterone once a day for 2 weeks. After the sex hormone administration, the EGFP expression in mouse liver was analyzed by IHC and quantified by ImageJ.
9
Metabolism of Therapeutic Drugs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Alprazolam, estradiol, midazolam, and testosterone were purchased from Fujifilm Wako Pure Chemicals (Osaka, Japan). Nifedipine, oxidized Nifedipine, 1′-hydroxymidazolam, 4-hydroxymidazolam, 4-hydroxyalprazolam, 2-/16α-hydoxyestradiol, and 6β-hydroxytestosterone were purchased from Sigma-Aldrich (St. Louis, MO). Pooled liver microsomes from dogs and humans were obtained from Corning Life Sciences (Woburn, MA) and intestinal microsomes were purchase from Sekisui Xenotech (Kansas City, KS). Recombinant human CYP3A4 in the bicistronic system with human NADPH-P450 reductase in bacterial membranes was prepared as described previously (Yamazaki et al., 2002) . Oligonucleotides were synthesized by Integrated DNA Technologies (Coralville, IA). All other reagents were purchased from Sigma-Aldrich or Fujifilm Wako Pure Chemicals, unless otherwise specified.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!