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Apc conjugated anti cd83 and phycoerythrin pe conjugated anti cd86 mabs

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APC-conjugated anti-CD83 and phycoerythrin (PE)-conjugated anti-CD86 mAbs are two monoclonal antibodies used for flow cytometry analysis. The anti-CD83 antibody is conjugated to the fluorescent dye allophycocyanin (APC), while the anti-CD86 antibody is conjugated to the fluorescent dye phycoerythrin (PE). These antibodies can be used to identify and quantify cells expressing the CD83 and CD86 surface markers.

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Lab products found in correlation

Raltegravir, by Thermo Fisher Scientific (2 mentions) Pe conjugated anti cd40l mab, by BD (2 mentions) Human inflammatory cytokine cytometric bead array, by BD (2 mentions)

Close spelling variants of this product

PE)-conjugated anti-CD83 APC-conjugated anti-CD86 Anti-CD86 mAb PE-conjugated anti-CD86 APC-conjugated CD86 Anti-CD83-APC PE-conjugated CD86 Anti-CD83-PE Anti-CD86-APC Anti-CD86-PE

2 protocols using apc conjugated anti cd83 and phycoerythrin pe conjugated anti cd86 mabs

1

Dendritic Cell Activation and Cytokine Profiling

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DCs (2.0 × 105) were plated in a 96 well dish and infected with titered lentiviral vector stock at a multiplicity of infection (MOI) of 2 in the presence or absence of 10 μM raltegravir (Fisher, Waltham, MA) or 1 μM nevirapine. After 72 hours, cells were stained with PE-conjugated anti-CD40L mAb (BD Biosciences, San Jose, CA) and the GFP+ and CD40L+ cells were quantified by flow cytometry. The differentiation state of the DCs was determined 72 hours post-infection by staining with APC-conjugated anti-CD83 and phycoerythrin (PE)-conjugated anti-CD86 mAbs (BioLegend, San Diego, CA) and measuring supernatant IL-12p70, TNF-α, IL-6, IL-1β, and IL-10 using the Human Inflammatory Cytokine Cytometric Bead Array (BD Biosciences).
Norton T.D., Miller E.A., Bhardwaj N, & Landau N.R. (2015). Vpx-containing Dendritic Cell Vaccine Vectors Induce CTLs and Reactivate Latent HIV-1 in vitro. Gene therapy, 22(3), 227-236.
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2

Dendritic Cell Activation and Cytokine Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
DCs (2.0 × 105) were plated in a 96 well dish and infected with titered lentiviral vector stock at a multiplicity of infection (MOI) of 2 in the presence or absence of 10 μM raltegravir (Fisher, Waltham, MA) or 1 μM nevirapine. After 72 hours, cells were stained with PE-conjugated anti-CD40L mAb (BD Biosciences, San Jose, CA) and the GFP+ and CD40L+ cells were quantified by flow cytometry. The differentiation state of the DCs was determined 72 hours post-infection by staining with APC-conjugated anti-CD83 and phycoerythrin (PE)-conjugated anti-CD86 mAbs (BioLegend, San Diego, CA) and measuring supernatant IL-12p70, TNF-α, IL-6, IL-1β, and IL-10 using the Human Inflammatory Cytokine Cytometric Bead Array (BD Biosciences).
Norton T.D., Miller E.A., Bhardwaj N, & Landau N.R. (2015). Vpx-containing Dendritic Cell Vaccine Vectors Induce CTLs and Reactivate Latent HIV-1 in vitro. Gene therapy, 22(3), 227-236.
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