Agilent 6890n network gc system

The ground resin (4.0 g) was extracted by maceration in MeOH (3 × 100 mL) at room temperature for 24 hours. After filtration, the organic solutions were concentrated at 40°C to give a crude MeOH extract that was diluted with H₂O (50 mL) and sequentially partitioned with n-hexane (3 × 50 mL) and CH₂Cl₂ (3 × 50 mL). The hexane fraction was evaporated under a vacuum, obtaining 0.90 g of extract. An amount of the hexane fraction was phytochemically studied and results have already been reported.^{15 (link)}The extract was further characterized by a Gas chromatography-mass spectometry (GC-MS) fingerprint chromatogram: GC analyses were performed on an Agilent 6890N Network GC System, equipped with a flame ionization detector and a DB-35ms column (30 m × 0.25 mm id, 0.25 µm film thickness). The oven temperature was programmed from 40°C for 10 minutes, then ramped at 5°C/min to 300°C and held for 10 minutes. Injector and detector temperatures were 250°C and 270°C, respectively. The sample was injected in the splitless mode using helium as carrier gas (1 mL/min); the samples were dissolved in DCM to give a 0.125-µL/mL solution; the injection volume was 1 µL (Figure 1).