Hepatoma cell lines FLC-4 were provided by Dr S. Nagamori (Kyorin University, Japan). Breast adenocarcinoma cell lines, MCF7, and gastric adenocarcinoma cell lines, AGS, were provided by the Department of Chest, Breast and Endocrine Surgery, Akita University, Japan. Colon carcinoma cell lines, HCT116, and T-cell leukemia cell lines, Jurkat, were provided by Dr H. Tomoda (Kitasato University, Japan). Esophageal carcinoma cell lines, TE4, and T-cell leukemia cell lines, HPB-ALL, HUT78 and PEER, were obtained from Riken Bioresource Center, Japan. Embryonic kidney cell lines, HEK293, were provided by the Department of Hematology, Nephrology and Rheumatology, Akita University. Promyelocytic leukemia cell lines, HL-60, and T-cell leukemia cell lines, JKT-beta-del, were obtained from the Japanese Collection of Research Bioresources, Japan. FLC-4, MCF7 and HCT116 cells were cultured in DMEM/F12, Eagle's MEM containing 10 μg/mL insulin and McCoy's 5A, respectively. AGS and HEK293 cells were cultured in DMEM. TE4, Jurkat, HPB-ALL, HUT78, PEER, HL-60 and JKT-beta-del cells were cultured in PRMI-1460. Each medium was supplemented with 10% FBS, 100 units/mL penicillin, 100 μg/mL streptomycin and 2.5 μg/mL amphotericin B. Cells were cultured in a humidified 5% CO2 atmosphere at 37°C.
Hl 60
Manufactured by Japanese Collection of Research Bioresources Cell Bank
Sourced in Japan
HL-60 is a human promyelocytic leukemia cell line. It is a suspension cell line derived from a patient with acute promyelocytic leukemia. HL-60 cells are commonly used in biomedical research to study cellular processes and evaluate drug efficacy.
Automatically generated - may contain errors
Lab products found in correlation
Thp 1, by Japanese Collection of Research Bioresources Cell Bank (3 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Hut78, by RIKEN BioResource Center (1 mentions)
Hl 60, by Thermo Fisher Scientific (1 mentions)
Mv4 11, by Thermo Fisher Scientific (1 mentions)
Hmc 1, by Thermo Fisher Scientific (1 mentions)
Ball 1, by Thermo Fisher Scientific (1 mentions)
Tall 1, by Thermo Fisher Scientific (1 mentions)
Thp 1, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Mv4 11, by American Type Culture Collection (1 mentions)
Ham s f 12 nutrient mixture, by Thermo Fisher Scientific (1 mentions)
Roswell park memorial institute 1640 medium, by Thermo Fisher Scientific (1 mentions)
Meg 01, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Jurkat, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Geneprint 10 system, by Promega (1 mentions)
Stemspan medium, by STEMCELL (1 mentions)
Lymphoprep gradient, by STEMCELL (1 mentions)
Mycoalert plus mycoplasma detection kit, by Lonza (1 mentions)
8 protocols using hl 60
1
Culturing of Human Cell Lines
2
Cultivation of Leukemia Cell Lines
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Leukemia cell lines, BALL-1, HL-60, TALL-1, KG-1, THP-1, Kasumi, and K562 were purchased from Japanese Collection of Research Bioresources Cell Bank (National Institutes of Biomedical Innovation, Health and Nutrition, Japan). HMC-1, MV4-11, Ba/F3 and 293T cells were purchased from American Tissue Culture Collection (Manassas, VA). BALL-1, HL-60, TALL-1, KG-1, THP-1, Kasumi, K562, and HMC-1 cells were cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA) supplemented with 10% fetal calf serum (FCS). Ba/F3 cells were cultured in RPMI 1640 medium supplemented with 10% FCS in the presence of 10 ng/ml IL-3. MV4-11 cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM, Thermo Fisher Scientific) supplemented with 10% FCS. 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific) with 10% FCS.
3
Cell Line Maintenance Protocols for Leukemia Research
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The human leukemia cell lines K562 [28 (link)], HEL [29 (link)], MEG–01 [30 (link)], CMK-11-5 [31 (link)], HL60 [32 (link)], NB4 [33 (link)], Jurkat [34 (link)], Reh [35 (link)], and THP–1 [36 (link)] were obtained from the Japanese Collection of Research Bioresources Cell Bank and maintained in Roswell Park Memorial Institute 1640 medium (Life Technologies) supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere of 5% CO2, unless otherwise indicated. The erythropoietin-dependent erythroid cell line UT–7/Epo was kindly provided by Dr. Norio Komatsu and maintained in the same medium supplemented with 10% FBS and 1 U/ml recombinant human erythropoietin (Kyowa Hakko Kirin, Tokyo, Japan) [37 (link)]. The Chinese hamster ovary cell line CHO-TRVb, which lacks functional TFR1, was kindly provided by Dr. Timothy McGraw and maintained in Ham’s F12 nutrient mixture (Life Technologies) supplemented with 5% FBS [38 (link)].
4
Authentication and Validation of AML Cell Lines
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Human AML cell lines human promyelocyte leukemia cell line (HL‐60) and KO52 (JCRB Cell Bank), U937 American Type Culture Collection (ATCC), human monocytic leukemia cell line (THP‐1) (ECACC), MARIMO (kind gift from Dr. Yosuke Minami at the Department of Hematology/Oncology, Kobe University, Kobe, Japan), and NB4 (kind gift from Dr. Hideki Nakakuma at the Department of Hematology/Oncology, Wakayama Medical University, Wakayama, Japan) were cultured as previously described [19 (link), 20 (link)]. The feeder cell line Hs27 was obtained from ATCC. All cell lines were cultured as previously described [19 (link), 20 (link)]. To authenticate cell lines, short tandem repeat analysis was performed by BEX using GenePrint 10 System (Promega). Mycoplasma testing was carried out routinely using an e‐Myco Plus Mycoplasma polymerase chain reaction (PCR) Detection Kit (iNtRON Biotechnology), and all cell lines were negative for mycoplasma.
5
Culturing AML Cell Lines and Primary Cells
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Human AML cell lines, KG-1, HL-60, PL-21, and Kasumi-3 cells, were obtained from Japanese Collection of Research Bioresources (JCRB) Cell Bank (Osaka, Japan) and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 µg/mL streptomycin at 37 °C, 5% CO2 and 95% relative humidity. Mycoplasma contamination was routinely tested by using MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza, Cologne, Germany), and any cell lines found positive were discarded. Patient-derived primary AML cells were obtained from PBMCs of relapsed patients, which were enriched over a Lymphoprep gradient (STEMCELL Technologies, Vancouver, Canada) (see Additional file 1 : Table S1 for clinical characterization). The primary AML cells were then cultured in StemSpan medium (STEMCELL Technologies) supplemented with 10 ng/mL interleukin (IL)-3, IL-6 and granulocyte colony-stimulating factor (G-CSF), 25 ng/mL thrombopoietin (TPO), 50 ng/mL stem cell factor (SCF) and FLT3 ligand, which promoted the growth and expansion of LSCs (Additional file 1 : Table S2). PBMCs from healthy donors were used as normal blood cells.
6
Cell Line Culture Protocols for Gastric and Lung Cancer Research
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Human GC cell lines HGC27, GSU, MKN1, and KATOIII, a human immortalized fetal lung fibroblast cell line L23immo, and a human pancreatic carcinoma cell line Panc-1 were obtained from RIKEN BioResource Research Center (BRC) (Japan). A human GC cell line NUGC-3, a human monocytic leukemia cell line THP-1, a human promyelocytic leukemia cell line HL60, and a human lung adenocarcinoma cell line A549 were obtained from Japanese Collection of Research Bioresources (JCRB) Cell Bank (Japan). Cells were cultured in RPMI1640 medium (#189-02025, FUJIFILM Wako Pure Chemical Corporation, Japan) supplemented with 10% fetal bovine serum (FBS) (#172012, Sigma-Aldrich) (with exceptions of KATOIII and HL60 that were cultured with 20% FBS), 2 mM L-glutamine (#073-05391, FUJIFILM Wako Pure Chemical Corporation), Penicillin/Streptomycin (#168-23191, FUJIFILM Wako Pure Chemical Corporation), and 1 mM sodium pyruvate (#11360-070, Thermo Fisher Scientific). Negativity for Mycoplasma infection has been regularly confirmed using TaKaRa PCR Mycoplasma Detection Set (TaKaRa Bio, Japan).
7
Cell line cultivation and authentication
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Leukemia cell lines, LOUCY, MEGAL, and OCI-AML3 were purchased from DSMZ (Braunschweig, Germany). K562, HL60, and HEL cells were obtained from JCRB cell bank (Ibaraki, Osaka). These cell lines were authenticated using STR typing by DSMZ or JCRB cell bank, and also the mycoplasma status was checked by them. LOUCY, MEGAL, and HL60 cells were cultured in RPMI1640 medium (Sigma) supplemented with 20% FBS (Sigma). K562 and HEL cells were cultured in RPMI1640 medium supplemented with 10% FBS. OCI-AML3 was cultured in alpha-MEM medium (WAKO) supplemented with 20% FBS. EB3 ES cells (Niwa et al., 2002 (link); Ogawa et al., 2004 (link)), E14tg2a ES cells (Hooper et al., 1987 (link)) and their derivatives, and HeLa cells were cultured as described previously (Oka et al., 2016 (link)).
8
Myeloma and Leukemia Cell Lines Study
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Human myeloma-derived cell lines (HMCLs), KMS-11, KMS-21-BM, KMS-26, and acute myelogenous leukemia-derived cell line HL-60 were purchased from the Japanese Collection of Research Bioresources (Osaka, Japan). BM samples were obtained from patients with MGUS (n = 12), NDMM (n = 23), and RRMM (n = 35) between September 2017 and March 2021 at the Division of Hematology and Oncology, Department of Medicine, Kyoto Prefectural University of Medicine (KPUM). MGUS/MM was diagnosed based on the International Myeloma Working Group 2014 criteria [19 (link)]. Written informed consent was obtained from all patients. The study was conducted in compliance with the Declaration of Helsinki, and the study protocol was approved by the institutional review board of KPUM (ERB-C-930-1).
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