Lc msd sl 1100 instrument

All solvents were purified before use. Ethyl chlorophormiate, pyrrolidine, morpholine, piperidine, acetophenone, ethyl oxalate, 2-acetylthiophene, and 2-acetylfurane were purchased from Acros Organics and used without purification. Reactions were monitored by thin-layer chromatography (TLC) using Fluka silica gel (60 F 254) plates (0.25 mm). Visualization was made with UV light. Melting points of the synthesized compounds were taken on a melting point tube. ¹H-NMR spectra were recorded on the Varian Gemini 400 MHz (Germany) in DMSO-d6 using tetramethylsilane (TMS) as an internal standard. Chemical shifts are reported in ppm units with use of the d scale. The mass spectra were recorded on an Agilent LC/MSD SL 1100 instrument (USA).

All solvents were purified before use. N-bromosuccinimide, thiourea, 2-methylbenzyl chloride, 3-fluorobenzyl chloride, 2-chlorobenzyl chloride, 2,4-dichlorobenzyl chloride, 3,4-dichlorobenzyl chloride, 4-bromobenzyl chloride, 2-chloroacetamide, and ethyl chloroacetate were purchased from Acros Organics and used without purification. Compounds 2, 3, 4b, and 4h were prepared using methods described previously [10 ]. The reactions were monitored by thin-layer chromatography (TLC) using Fluka silica gel (60 F 254) plates (0.25 mm). Visualization was made with UV light. The melting points of the synthesized compounds were taken on a melting point tube. Infrared spectra were recorded on a Bruker Tensor 37 spectrometer (Germany). The ¹H-NMR spectra were recorded on a Bruker WP 250 SY spectrometer (250.13 MHz) (Germany). Chemical shifts are reported relative to chloroform (δ=7.25 ppm) for ¹H-NMR. The mass spectra were recorded on an Agilent LC/MSD SL 1100 instrument (USA).