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Nrf2 ep1808y mab

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NRF2 (EP1808Y) mAb is a monoclonal antibody that recognizes the nuclear factor erythroid 2-related factor 2 (NRF2) protein. NRF2 is a transcription factor that regulates the expression of antioxidant response element-driven genes, which play a role in the cellular defense against oxidative stress.

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Lab products found in correlation

Nitrocellulose membrane, by Merck Group (2 mentions) Histone extraction kit, by Abcam (2 mentions) Total h3, by Cell Signaling Technology (2 mentions) Nupage gel, by Thermo Fisher Scientific (2 mentions) Nqo1 hpa007308 and phgdh hpa021241, by Merck Group (2 mentions) H3k4me3, by Cell Signaling Technology (2 mentions) H3k27me3, by Merck Group (2 mentions)

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EP1808Y (9 citations)

2 protocols using nrf2 ep1808y mab

1

Western Blot Analysis of Stress Responses

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Protein lysates were prepared using RIPA lysis buffer and separated on 4–12% NuPAGE gels (Invitrogen), transferred onto a nitrocellulose membrane (Millipore) and incubated with the following antibodies: ACTIN (ab6276) and NRF2 (EP1808Y) mAb (both Abcam), NQO1 (HPA007308) and PHGDH (HPA021241) (both Sigma), PSAT1 (PA5-22124, Pierce), and ATF4 (11815, Cell Signaling). Alternatively, nuclear extracts were prepared as described31 . Histone extracts were prepared with the Histone Extraction Kit according to the manufacturer’s instructions (Abcam, ab113476). Histone extracts were probed with the following antibodies: H3K4me3 (9727, Cell Signaling), H3K27me3 (07-449, Millipore), total H3 (4499, Cell Signaling).
DeNicola G.M., Chen P.H., Mullarky E., Sudderth J.A., Hu Z., Wu D., Tang H., Xie Y., Asara J.M., Huffman K.E., Wistuba I.I., Minna J.D., DeBerardinis R.J, & Cantley L.C. (2015). NRF2 regulates serine biosynthesis in non-small cell lung cancer. Nature genetics, 47(12), 1475-1481.
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2

Western Blot Analysis of Stress Responses

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein lysates were prepared using RIPA lysis buffer and separated on 4–12% NuPAGE gels (Invitrogen), transferred onto a nitrocellulose membrane (Millipore) and incubated with the following antibodies: ACTIN (ab6276) and NRF2 (EP1808Y) mAb (both Abcam), NQO1 (HPA007308) and PHGDH (HPA021241) (both Sigma), PSAT1 (PA5-22124, Pierce), and ATF4 (11815, Cell Signaling). Alternatively, nuclear extracts were prepared as described31 . Histone extracts were prepared with the Histone Extraction Kit according to the manufacturer’s instructions (Abcam, ab113476). Histone extracts were probed with the following antibodies: H3K4me3 (9727, Cell Signaling), H3K27me3 (07-449, Millipore), total H3 (4499, Cell Signaling).
DeNicola G.M., Chen P.H., Mullarky E., Sudderth J.A., Hu Z., Wu D., Tang H., Xie Y., Asara J.M., Huffman K.E., Wistuba I.I., Minna J.D., DeBerardinis R.J, & Cantley L.C. (2015). NRF2 regulates serine biosynthesis in non-small cell lung cancer. Nature genetics, 47(12), 1475-1481.
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