Height and body weight were measured using a digital scale, and body mass index (BMI) was calculated as body weight (kg)/height squared (m2). Waist circumference was measured at the umbilical level by a well-trained nurse. Systolic blood pressure and diastolic blood pressure were measured after 15 min rest. The blood sample for laboratory tests was drawn from individuals after 8 h or more of fasting, and tests included the following: serum fasting blood glucose (FBG), total cholesterol (TC), triglycerides (TG), low-density-lipoprotein cholesterol (LDL-C), high-density-lipoprotein cholesterol (HDL-C), creatinine, and uric acid (UA); serum calcium, albumin, and phosphorus were measured using a Hitachi 7600 clinical analyzer (Hitachi, Tokyo, Japan). Serum thyroid-stimulating hormone (TSH), thyroxine (FT4), free triiodothyronine (FT3), thyroid peroxidase antibody, (TPO-Ab) and thyroid globulin antibody (Tg-Ab) were quantified using chemiluminescent enzyme immunoassays (ICMA; Abbott, Chicago, IL, USA). Serum 25(OH)D concentration was measured with radioimmunoassay and automeasured by using a Roche cobas 8000 automatic biochemical analyzer.
Cobas 8000 automatic biochemical analyzer
Manufactured by Roche
Sourced in Switzerland, Germany
The Cobas 8000 is an automatic biochemical analyzer designed for clinical laboratory settings. It performs various clinical chemistry tests using a variety of sample types, including blood, serum, and urine. The Cobas 8000 is capable of high-throughput analysis, with the ability to process a large number of samples efficiently. The system is designed to provide accurate and reliable results to support clinical decision-making.
Automatically generated - may contain errors
Lab products found in correlation
7600 clinical analyzer, by Hitachi (1 mentions)
Hematology analyzer lh750, by Beckman Coulter (1 mentions)
Cobas e601 immunoassay analyzer, by Roche (1 mentions)
Cal 8000 blood cell analyzer, by Mindray (1 mentions)
Bc 6900 automatic blood cell analyzer, by Mindray (1 mentions)
Advia 2120i automatic blood analyzer, by Siemens (1 mentions)
Hcy reagent, by Beijing Strong Biotechnologies (1 mentions)
Lal assay kit, by Lonza (1 mentions)
13 protocols using cobas 8000 automatic biochemical analyzer
1
Comprehensive Metabolic and Anthropometric Assessment
2
Stroke Biomarker Evaluation Protocol
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Laboratory tests were obtained within 24 h of admission after stroke onset, including the serum albumin, hemoglobin, lymphocyte, and platelet levels. The blood counts were analyzed using an autoanalyzer (Beckman Coulter Hematology Analyzer LH750, USA). All the serum biochemical parameters were assayed using an automatic biochemical analyzer (Roche COBAS 8000 automatic biochemical analyzer). The HALP score was calculated according to the following formula: hemoglobin (g/L) × albumin (g/L) × lymphocytes (/L)/platelets (/L).
3
Hormonal and Lipid Profiling
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Fasting serum specimens were collected. Serum FSH, luteinizing hormone (LH) and estradiol (E2) were detected by electrochemical luminescence with a Cobas e 601 immunoassay analyzer (Roche, Basel, Switzerland). Fasting blood glucose, triglycerides (TG), total cholesterol (TC), high‐density lipoprotein cholesterol (HDL‐C) and low‐density lipoprotein cholesterol (LDL‐C) were detected by enzyme colorimetry using a Cobas 8000 automatic biochemical analyzer (Roche).
4
Comprehensive Hematology Profiling
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(1) before thrombolysis, 2 mL of venous blood was collected with EDTA-K2 anticoagulant vacuum tube. Within 30 minutes after reversing evenly, it was detected with automatic Mindray cal 8000 blood cell analyzer. (2) Before thrombolysis, collect 3~5 mL of venous blood with a coagulation promoting tube, centrifuge at 3500 rpm for 10 min, use Roche Cobas 8000 automatic biochemical analyzer to detect albumin, creatinine, urea nitrogen, blood glucose and other indicators, and use Roche diagnostic reagents to complete the detection within 4 hours. (3) Before thrombolysis, use sodium citrate anticoagulant vacuum tube to collect 3 mL of venous blood, centrifuge at 3500 rpm for 15 min, separate plasma, use STAGO compact Max Automatic Coagulation Analyzer to detect coagulation function indexes, and use STAGO matching reagent to complete the detection within 2 h.
5
Hepatic Biochemical Indices Assessment
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The levels of hepatic biochemical indices, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and albumin (ALB), were determined by ELISA kits (Roche; Shanghai). These indices were detected every two weeks to estimate the extent of liver injury. AST and ALT mainly exist in hepatic cells. Their levels were detected in blood when more hepatic cells were damaged. ALB was detected by the bromocresol green method. Sample blood was used to examine hepatic biochemical indices within 2 h after collection to ensure the reliability of the results by the Roche Cobas 8000 automatic biochemical analyzer. The liver function indices measured in the second, fourth and sixth weeks are shown in Supplementary Table 1 , and the liver function index results from the eighth week are shown in Supplementary Table 2 .
6
Biomarkers in Colorectal Liver Metastasis
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Patients diagnosed with CRLM were screened using a blood test before receiving drug therapy for CRLM. For blood cell testing, including measuring levels of ApoA-I and NLR, 5 ml of fasting venous blood was taken from all patients and placed in K2-EDTA disposable anticoagulant vacuum tubes. After mixing, blood was collected for examination. ApoA-I was detected using a Cobas 8000 automatic biochemical analyzer (Roche). The NLR was tested using a BC-6900 automatic blood cell analyzer (Mindray, Shenzhen).
Through the hospital's electronic medical record system, the duration of follow-up was established to be from the first day of hospitalization to December 31, 2021. The variables (ApoA-I and NLR) were classified using the median value as the cut-off point. Thus, the cut-off level for ApoA-I was 1.03 g/L, and NLR was 3.24. We used the ApoA-I and NLR to analyze whether they were clinically associated with CRLM.
Through the hospital's electronic medical record system, the duration of follow-up was established to be from the first day of hospitalization to December 31, 2021. The variables (ApoA-I and NLR) were classified using the median value as the cut-off point. Thus, the cut-off level for ApoA-I was 1.03 g/L, and NLR was 3.24. We used the ApoA-I and NLR to analyze whether they were clinically associated with CRLM.
7
Comprehensive Stroke Treatment Evaluation
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The patients' basic data, including sex, age, HbA1c (HPLC, PRIMUS Ultra2 glycosylated hemoglobin analyzer); glucose, homocysteine (HCY), total cholesterol (TC), and triglyceride (TG) levels; and laboratory indexes were measured using a German Roche Cobas8000 automatic biochemical analyzer. The treatment onset time; length of hospital stay; history of smoking, drinking, heart disease, stroke, or hypertension; NIHSS score at admission and discharge; and complications (bleeding, hyperperfusion, or reinfarction) were also recorded. The surgical
8
Comprehensive Hepatic Biomarker Profiling
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Serum total bilirubin (TBIL), direct bilirubin (DBIL), indirect bilirubin (IBIL), aspartate transaminase (ALT), glutamic-oxalacetic transaminase (AST), glutamyltranspeptidase (GGT), apolipoprotein B (APO-B), and low-density lipoprotein cholesterol (LDL-C) were measured using a cobas8000 automatic biochemical analyzer (Roche Diagnostics, Switzerland). Serum hepatitis B surface antigen (HBsAg), anti-HBs, HBeAg, anti-HBe, and anti-HBc were detected using the Architect ci4100 automatic biochemical immunoassay system (Abbott Laboratories, USA). Serum HBV DNA was quantified on the Roche LightCycler 480 system (Roche Corporation, Switzerland) using a quantitative real-time PCR (qRT-PCR) assay (Sansure Biotech, China). Complete blood counts were performed using the Advia 2120i automatic blood analyzer (Siemens, Germany).
9
Comprehensive Metabolic Profile Assessment
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Hemoglobin (Hb) was detected by a XE-5000 automatic blood analyzer (Japan), and fasting plasma glucose (FPG), ALB, cholesterol, triglyceride (TG), blood uric acid, blood creatinine, high density lipoprotein-c (HDL-c), low density lipoprotein-c (LDL-c), alanine aminotransferase, aspartate aminotransferase, total bilirubin, direct bilirubin and cystatin C (Cys-C) were detected by a cobas 8000 automatic biochemical analyzer (Roche, Germany). The ratio of TG and cystatin C was calculated. The glycosylated hemoglobin (HbA1C) was detected by high-performance liquid exchange chromatography with a HCL-723G8 analyzer (Japan), and fibrinogen was determined by the coagulation method with an automated hemagglutination test (Germany). The estimated GFR (eGFR) was estimated using the formula of kidney disease diet improvement (MDRD).7 (link)
10
Postoperative Kidney Function Monitoring
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Postoperative serum and urine samples were collected from patients on an empty stomach in the morning. Samples on postoperative day 1 were collected nearly 3 to 12 hours after renal transplantation. The samples were collected daily from day 1 to day 7 after kidney transplantation.
Serum creatinine was measured by the creatinine oxidase method; blood urea nitrogen and uric acid (UA) were detected by the colorimetric method; cystatin C (Cys C) and β2‐microglobulin (β2‐MG) were measured by the immunoturbidimetric method; estimated glomerular filtration rate (eGFR) was calculated using the modification of diet in renal disease formula.12 Roche cobas 8000 automatic biochemical analyzer (Roche Diagnostics, Switzerland) and test kits (Roche Diagnostics, Switzerland) were used to detect daily renal function markers, and all experimental procedures strictly followed the manufacturer's instructions. Immunological scattering turbidity was used to detect serum and urinary NGAL using the PA8800 special protein analyzer (Beijing Prom Medical, China), according to the neutrophil gelatinase‐related lipocalin detection kit (Beijing Prom Medical). Urinary NGAL >100 ng/mL or serum NGAL concentration >106 ng/mL was judged as positive, according to the reference value provided by the manufacturer.
Serum creatinine was measured by the creatinine oxidase method; blood urea nitrogen and uric acid (UA) were detected by the colorimetric method; cystatin C (Cys C) and β2‐microglobulin (β2‐MG) were measured by the immunoturbidimetric method; estimated glomerular filtration rate (eGFR) was calculated using the modification of diet in renal disease formula.
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