Three tumor tissues were randomly selected from each group for Western blot analysis, according to the methods described in our previous study.39 (link) Primary antibodies used were as follows: S1P (Bioss, Beijing, China); S1PR5 (Proteintech, Wuhan, China); GNAI1 (Proteintech, Wuhan, China); Rac1 (Proteintech, Wuhan, China); KRAS (Proteintech, Wuhan, China); ERK1/2 (Proteintech, Wuhan, China); RAF1 (Proteintech, Wuhan, China); Phospho-ERK1/2 (Proteintech, Wuhan, China); MEK1 (Santa, USA); Phospho-RAF1 (Abcam, USA); Phospho-MEK1 (Abcam USA); PI3K p85 alpha (Proteintech, Wuhan, China), Phospho-PI3K p85 alpha (Abcam USA); RAC1 (Proteintech, Wuhan, China); GAPDH (Proteintech, Wuhan, China).
Phospho mek1
Manufactured by Abcam
Sourced in United States
Phospho-MEK1 is a lab equipment product that is used to detect and quantify the phosphorylation state of MEK1 protein. It provides a reliable and accurate method for researchers to study cell signaling pathways involving the MEK1 protein.
Automatically generated - may contain errors
Lab products found in correlation
Raf 1, by Proteintech (1 mentions)
Phospho erk1 2, by Proteintech (1 mentions)
Erk1 2, by Proteintech (1 mentions)
Gapdh, by Proteintech (1 mentions)
Protein phosphatase inhibitor, by Solarbio (1 mentions)
Phospho erk1 2, by Abcam (1 mentions)
Phospho fak, by Abcam (1 mentions)
Ripa buffer, by Beyotime (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Solarbio (1 mentions)
Bca protein assay kit, by Solarbio (1 mentions)
Erk1 2, by Abcam (1 mentions)
Protease inhibitor, by Solarbio (1 mentions)
2 protocols using phospho mek1
1
Western Blot Analysis of Tumor Tissues
2
Western Blot Analysis of Focal Adhesion Proteins
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Cells were lysed in RIPA buffer (Beyotime) containing protease inhibitor PMSF (Solarbio) at ratio (100:1 = RIPA: PMSF) and protein phosphatase inhibitor (Solarbio), then clarified by centrifugation at 13,000 g for 10 min at 4°C. Protein concentration was quantified using BCA Protein Assay Kit (Solarbio, Beijing, China). Western blot was performed by automatic protein analyser (Wes&Jess) according to the manufacturer's instruction. All protein levels were normalized to β‐actin. The blots were reacted with rabbit anti‐human against β‐actin (CST), phospho‐FAK, FAK, phospho‐MEK1, MEK1, phospho‐ERK1/2, ERK1/2, β3, αv, α5, β1 (all from Abcam).
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