Human PC cell lines PANC-1, CFPAC-1, and Patu8988 (ATCC, Manassas, VA, USA) were grown in Dulbecco’s modified Eagle medium (DMEM; Invitrogen, Carlsbad, CA) that contained 10% fetal bovine serum and 1% penicillin–streptomycin (Sigma, Taufkirchen, Germany). Primary antibodies for NUDCD1 (Ab126902, 1:1000) and GAPDH (Ab8245, 1:2000) were obtained from Abcam. Antibodies against human Bcl-2 (12789-1-AP, 1:1000), Bax (50599-2-Ig, 1:1000) and Caspase 3 (19677-1-AP, 1:1000) were purchased from Proteintech. Antibodies against vimentin (AF7013, 1:1000), N-cadherin (AF4039, 1:1000), Cleaved-Caspase 3 (AF7022, 1:1000) and E-cadherin (AF0131, 1:1000) were purchased from Affinity Biosciences.
Ab126902
Manufactured by Abcam
Sourced in United States
Ab126902 is a monoclonal antibody specifically designed to recognize and bind to the target protein. This product is intended for research use only.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified, by Thermo Fisher Scientific (1 mentions)
E cadherin, by Affinity Biosciences (1 mentions)
Cfpac 1, by American Type Culture Collection (1 mentions)
Cleaved caspase 3, by Affinity Biosciences (1 mentions)
Patu8988, by American Type Culture Collection (1 mentions)
N cadherin, by Affinity Biosciences (1 mentions)
Panc 1, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Ab8245, by Abcam (1 mentions)
Ab252833, by Abcam (1 mentions)
Ab75972, by Abcam (1 mentions)
Protein extraction kit, by Solarbio (1 mentions)
2 protocols using ab126902
1
Protein Expression in Pancreatic Cancer Cells
2
Investigating Protein Expression in Liver Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total protein from L-02, SMMC7721, or HepG2 cells was extracted using a protein extraction kit (Solarbio, China). The protein concentration was quantified with the Bradford approach. Protein samples (50 µg) were separated via denaturing sodium dodecyl sulfate-polyacrylamide gel electrophoresis, followed by transference onto polyvinylidene fluoride membranes. Membranes were blocked by 5% skim milk then incubated with primary antibodies targeting NUDCD1 (1/5,000; ab126902; Abcam, USA), GAPDH (1/5,000; ab199553), GPX4 (1/2,000; ab252833), and ferritin heavy chain 1 (FTH1) (1/2,000; ab75972), followed by a secondary antibody. Protein bands were developed with an enhanced chemiluminescence reagent.
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