Cal 520 am ab171868

Manufactured by Abcam

Sourced in United Kingdom

Cal‐520/AM (ab171868) is a fluorescent calcium indicator that can be used to measure changes in intracellular calcium levels. It has an excitation wavelength of 492 nm and an emission wavelength of 514 nm.

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Lab products found in correlation

Thrombin t4648, by Merck Group (1 mentions) Hc 067047, by Merck Group (1 mentions) Pluronic f 127 p3000mp, by Thermo Fisher Scientific (1 mentions) Collagenase type 2 ls004176, by Worthington (1 mentions) Uk5099 pz0160, by Merck Group (1 mentions)

Close spelling variants of this product

Cal‐520/AM (18 citations) Cal-520 (11 citations) Ab171868 (2 citations)

2 protocols using cal 520 am ab171868

Evaluating PAR1 and PAR2 Signaling

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The PAR activating peptides (>95% pure by HPLC) TFLLR‐NH₂ (PAR1) and 2‐furoyl‐LIGRLO‐amide (PAR2) were synthesized in the University of Calgary peptide synthesis facility. Acetylcholine (A6625), 2‐APB (D9754), Caffeine (C0750), phospholipase C (PLC) inhibitor—U73122 (U6756), TRPV4 blocker—HC 067047, CPA (C1530), phenylephrine (P6126), sodium nitroprusside (S0501), trypsin (T1426), and thrombin (T4648) were from Sigma UK. The stock solution of acetylcholine (ACh, 100 mM) and phenylephrine (10 mM) was prepared in MilliQ water. Sodium nitroprusside and caffeine were dissolved in PSS. 2‐APB and U73122 were dissolved in dimethyl sulfoxide (DMSO, D8418) and diluted in PSS to the final concentration. Cal‐520/AM (ab171868) was obtained from Abcam (UK). Pluronic F‐127 was obtained from SiChem (Germany).

Zhang X., Lee M.D., Buckley C., Hollenberg M.D., Wilson C, & McCarron J.G. (2023). Endothelial PAR2 activation evokes resistance artery relaxation. Journal of Cellular Physiology, 238(4), 776-789.

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Comprehensive Toolkit for Calcium Imaging and Mitochondrial Analysis

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Cal-520/AM (ab171868) and mitoTEMPO (ab144644) were purchased from Abcam (United Kingdom). Caged IP₃ (cag-iso-2–145–100) was purchased from SiChem (Germany). ACh (A6625), apamin (A1289), dihydroethidium (D7008), GSK1016790A (G0798), oligomycin (O4876), ouabain (O3125), L-NA (N5501), L-NAME (N5751), MSDC-0160 (mitoglitazone, SML1884), PE (P6126), propidium iodide (P4864), rotenone (R8875), SNP (S0501), TEMPOL (4-HYDROXY-TEMPO; 176141), TRAM-34 (T6700), and UK5099 (PZ0160) were purchased from Sigma-Aldrich (United States). Pluronic F127 (P3000MP) was purchased from ThermoFisher (United Kingdom). Collagenase (Type 2; LS004176) was purchased from Worthington (United States). All other chemicals were obtained from Sigma-Aldrich.

Wilson C., Lee M.D., Buckley C., Zhang X, & McCarron J.G. (2022). Mitochondrial ATP Production is Required for Endothelial Cell Control of Vascular Tone. Function, 4(2), zqac063.

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