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Mouse rat creatinine elisa kit

Manufactured by Cayman Chemical

The Mouse/Rat Creatinine ELISA Kit is a quantitative colorimetric assay designed for the measurement of creatinine levels in mouse and rat samples. The kit utilizes the ELISA (Enzyme-Linked Immunosorbent Assay) technique to detect and quantify creatinine concentrations.

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2 protocols using mouse rat creatinine elisa kit

1

Urine Albumin and Creatinine Quantification

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Spot urine was used to determine levels of albumin and creatinine using either mouse/rat albumin ELISA kit (Bethyl Laboratories, Montgomery, TX) or mouse/rat creatinine ELISA kit (Cayman Chemical, Ann Arbor, MI), respectively, in all animal models except transgenic DYN1R725A mice. Urine samples from Empty (podocine-only transgenic animals) and transgenic DYN1R725A mice were collected by placing them into xurine collection containers for 4 to 6 h. A physical barrier prevented the mixing of solid and liquid wastes, allowing urine samples to be collected free of fecal contamination. Samples were diluted to 1:5, 1:10, or 1:50 with distilled water before total protein was measured using a mouse albumin ELISA kit (Bethyl Laboratories, Montgomery, TX) and urine creatinine measurements were taken using an ELISA kit (Cayman Chemical, Ann Arbor, MI). Absorbances were measured using EnSpire 2300 Multilabel Reader (Perkin Elmer, Waltham, MA). No data points have been excluded. Data were plotted as mean ± SD and statistical significance was determined using unpaired t-test calculated using Prism software (GraphPad, La Jolla, CA).
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2

Urine Albumin and Creatinine Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spot urine was used to determine levels of albumin and creatinine using either mouse/rat albumin ELISA kit (Bethyl Laboratories, Montgomery, TX) or mouse/rat creatinine ELISA kit (Cayman Chemical, Ann Arbor, MI), respectively, in all animal models except transgenic DYN1R725A mice. Urine samples from Empty (podocine-only transgenic animals) and transgenic DYN1R725A mice were collected by placing them into xurine collection containers for 4 to 6 h. A physical barrier prevented the mixing of solid and liquid wastes, allowing urine samples to be collected free of fecal contamination. Samples were diluted to 1:5, 1:10, or 1:50 with distilled water before total protein was measured using a mouse albumin ELISA kit (Bethyl Laboratories, Montgomery, TX) and urine creatinine measurements were taken using an ELISA kit (Cayman Chemical, Ann Arbor, MI). Absorbances were measured using EnSpire 2300 Multilabel Reader (Perkin Elmer, Waltham, MA). No data points have been excluded. Data were plotted as mean ± SD and statistical significance was determined using unpaired t-test calculated using Prism software (GraphPad, La Jolla, CA).
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