Surface marker expression was analyzed by FACS Verse (BD) in synovial MSCs from four donors at passage 2. The cells before and 48 h after preservation were suspended in Hank’s balanced salt solution (HBSS) at a density of 5 × 105 cells/mL and stained for 30 min on ice with the antibodies CD44-PE-Cy7, CD73-V450, CD90-PE, CD105-APC, CD34-PE-Cy5, CD45-APC-H7, and CD31-FITC (all from BD), and Ghost Dye Violet 510 for dead cells (Tonbo Biosciences, CA, USA). These data were also analyzed using FlowJo software.
Cd73 v450
Manufactured by BD
Sourced in United States
The CD73-V450 is a laboratory equipment product. It is used for the detection and analysis of CD73, which is a cell surface enzyme involved in the production of adenosine. The product utilizes a V450 fluorescent dye to label CD73 for visualization and quantification purposes.
Automatically generated - may contain errors
Lab products found in correlation
Cd105 apc, by BD (2 mentions)
Cd90 pe, by BD (1 mentions)
Cd31 fitc, by BD (1 mentions)
Cd44 pe cy7, by BD (1 mentions)
Cd34 pe cy5, by BD (1 mentions)
Facsverse, by BD (1 mentions)
Cd45 apc h7, by BD (1 mentions)
Ghost dye violet 510, by Cytek Biosciences (1 mentions)
Cd34 pe, by BD (1 mentions)
Facsverse 2 system, by BD (1 mentions)
Cd44 apc h7, by BD (1 mentions)
Cd45 percp cy5, by BD (1 mentions)
Cd90 pe cy7, by BD (1 mentions)
2 protocols using cd73 v450
1
Characterizing Synovial MSC Phenotype
2
Characterization of Human Synovial MSCs
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Human synovial MSCs were detached with trypsin and suspended in PBS containing 2% FBS and 5 mM EDTA. The MSCs were then stained for 30 min at 4 °C with CD44-APCH7, CD73-V450, CD90-PE-Cy7, CD105-APC, CD34-PE, and CD45-PerCP-Cy5.5 antibodies (1:200; BD Biosciences), using Ghost Dye Violet (1:1000; Tonbo Biosciences, San Diego, CA, USA) to remove dead cells. Isotype controls were prepared as negative controls. The percentage of antigen-positive cells was evaluated using a FACSVerse II system (BD Biosciences).
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