OS cells with the applied genetic modifications were seeded into six-well plates (at 1 × 105 cells in each well) and cultured for 72 h. An EdU Apollo-567 assay kit (RiboBio, Guangzhou, China) was employed to quantitatively measure cell proliferation. The cell nuclei were stained with both EdU and DAPI, visualized under a fluorescent microscope (Leica, DM 4000, Germany). Cells in each field of view were then counted and analyzed. The nuclear EdU ratio, % versus DAPI, from at least 1,000 cells in five random views per treatment was calculated.
Edu apollo 567 assay kit
Manufactured by RiboBio
Sourced in China
The EdU Apollo-567 assay kit is a laboratory tool designed for the detection and quantification of cell proliferation. It utilizes a fluorescent detection method to label and analyze cells undergoing DNA synthesis, a key indicator of cell division and proliferation. The kit provides the necessary reagents and protocols for researchers to perform this analysis.
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8 protocols using edu apollo 567 assay kit
1
Cell Proliferation Assay with EdU-DAPI Staining
2
Quantifying Cell Proliferation with EdU Assay
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Cells were initially seeded into six-well plates (at 100,000 cells in each well). After the applied treatment, EdU Apollo-567 assay kit (RiboBio, Guangzhou, China)11 (link),12 (link) was applied to examine and quantify cell proliferation. The nuclear EdU and DAPI staining were visualized through a fluorescent microscope. Five random views with total 800–1000 cells of each treatment were included to calculate EdU/DAPI ratio.
3
Glioma Cell Proliferation Assay
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The transfected glioma cells were seeded onto the six-well tissue-culturing plates (1 × 105cells per well). Following the indicated treatments an EdU Apollo-567 assay kit (RiboBio, China) was utilized to test cell proliferation, with nuclear EdU and DAPI staining visualized under a fluorescent microscope.
4
Oxidative Stress and Cell Proliferation Assay
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MSCs were seeded into six-well plates (at 1 × 105 cells in each well) and were treated with H2O2 (500 μmol) and/or Gel in specified time. An Edu Apollo-567 assay kit (RiboBio, Guangzhou, China) was utilized to quantify cell proliferation. Briefly, cell nuclei were co-stained with Edu and DAPI for 3 h, visualized under a fluorescent microscope (Leica, DM 4000, Germany). Cells in each field of view were then counted and analyzed by the Photoshop software Edu (positive cells/nuclei).
5
Cell Proliferation Assay via EdU-DAPI Staining
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Cells were initially seeded into 24-well plates (2 x 104 cells/cm2). With the applied treatment, an EdU Apollo-567 assay kit (RiboBio, Guangzhou, China) was utilized to test cell proliferation. Cells were co-stained with EdU and DAPI, visualized under a fluorescent microscope (Leica). In each condition 600 cell nuclei in six random views were counted to calculate EdU/DAPI ratio [30 (link), 31 (link)].
6
Cell Proliferation Assay with EdU
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The primary human RCC cells or the epithelial cells were seeded onto the six-well tissue-culturing plates (1×105 cells per well). Following the indicated treatments an EdU Apollo-567 assay kit (RiboBio, Guangzhou, China) was utilized to test cell proliferation, with nuclear EdU and DAPI staining visualized under a fluorescent microscope (1×200 magnification, Leica, Shanghai, China.). In each treatment five random views with total 500 cells were included to calculate the nuclear EdU ratio (% vs. DAPI).
7
Quantifying Cell Proliferation via EdU Assay
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Human OS cells with the applied genetic modifications were seeded into six-well plates (at 1 × 105 cells in each well) and cultured for 48h. An EdU Apollo-567 assay kit (RiboBio, Guangzhou, China) was utilized to quantify cell proliferation. Briefly, cell nuclei were co-stained with EdU and DAPI for 3h, visualized under a fluorescent microscope (Leica, DM 4000, Germany)
8
Assessing cell proliferation by EdU
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As reported [14 (link)], cells with applied genetic modifications were initially seeded into six-well plates (1×105 cells per well) and cultured for 72h. Afterwards, an EdU Apollo-567 assay kit (RiboBio, Guangzhou, China) was utilized to stain proliferative nuclei (EdU-positive). Five random views under a fluorescent microscope (1000 nuclei per treatment) were used to calculate the average EdU/DAPI ratio (×100%).
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