Tissues were collected and fixed in 10% formalin and then were dehydrated and embedded in paraffin for sectioning. Sections (5 μm) were then deparaffinized and rehydrated for immune-histochemical staining with the primary or isotype control antibodies, followed by DAB substrate for visualization. All sections were counterstained with hematoxylin for nuclei staining. Information on all antibody reagents used is provided in Supplementary Note 1 . Images were taken by Leica DME brightfield microscope and processed by Leica Las EZ imaging software. To quantify cleaved caspase 3 staining in mouse tumors, 5 different fields were randomly picked from each mouse tumor and staining intensity was quantified by Fiji software (Fiji.sc).
Dme brightfield microscope
Manufactured by Leica
The DME brightfield microscope is a laboratory equipment designed for basic brightfield microscopy applications. It provides clear and high-quality images for sample observation and analysis.
Automatically generated - may contain errors
4 protocols using dme brightfield microscope
1
Immunohistochemical Analysis of Mouse Tumors
2
Formalin-Fixed Paraffin Embedding for Histology
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VAT was excised, immediately fixed in 10% formalin for 24 h, and then paraffin embedded. H&E staining was performed on 6-µm tissue sections by the Benaroya Research Institute Histology Core. Slides were imaged at RT with a Leica DM E brightfield microscope with a 10× eyepiece and 20× objective lens (together, 200×). Images were captured with a Leica EC3 3.1 megapixel digital color camera and imported into Leica Application Suite EZ imaging software.
3
Immunohistochemical Analysis of Mouse Tumors
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Tissues were collected and fixed in 10% formalin and then were dehydrated and embedded in paraffin for sectioning. Sections (5 μm) were then deparaffinized and rehydrated for immune-histochemical staining with the primary or isotype control antibodies, followed by DAB substrate for visualization. All sections were counterstained with hematoxylin for nuclei staining. Information on all antibody reagents used is provided in Supplementary Note 1 . Images were taken by Leica DME brightfield microscope and processed by Leica Las EZ imaging software. To quantify cleaved caspase 3 staining in mouse tumors, 5 different fields were randomly picked from each mouse tumor and staining intensity was quantified by Fiji software (Fiji.sc).
4
Tissue Fixation and Histological Analysis
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VAT was excised, immediately fixed in 10% formalin for 24 hr, then paraffin-embedded. Hematoxylin and eosin (H&E) staining was performed on 6 μm tissue sections by the Benaroya Research Institute Histology Core. Slides were imaged at room temperature with a Leica DM E brightfield microscope with a 10X eyepiece and 20X objective lens (together 200X). Images were caputured with a Leica EC3 3.1 megapixel digital color camera and imported into Leica Application Suite EZ imaging software.
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