Expression of GPC3 in Hepa1-6 cells was detected by western blot and fluorescence microscopy based on protocols reported in previous studies [56 (link)]. Cell nuclei were labeled with DAPI (4′, 6-diamidino-2-phenylindole, Sigma). The following antibodies were used in this section: GPC3 (middle region) antibody (Aviva Systems Biology Corp., San Diego, CA, rabbit, #ARP37665), anti-rabbit IgG-HRP antibody (Cell Signaling Technology, #7074); anti-GAPDH antibody (Thermo, mouse, #MA5-15738), anti-mouse IgG-HRP antibody (Cell Signaling Technology, #7076); GPC3 monoclonal (9C2) antibody (Thermo, mouse, #MA5-17083) and Alexa Fluor@488 donkey anti-mouse IgG (H+L) secondary antibody (Life Technologies, #1423052). Implanted HCC models were established with B6 mice (n = 4/group). Briefly, 5×105 Hepa1-6 cells were injected subcutaneously (or orthotopically) into the right flank (or the liver) of the mice. Two weeks later, the mouse HCC tissues were dissected. Immunohistochemical detection of GPC3 was performed as described previously [57 (link)]. All cell cultures were free of mycoplasma and maintained in complete medium (RPMI 1640 or DMEM supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin).
Anti mouse igg hrp antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Anti-mouse IgG-HRP antibody is a secondary antibody that binds to mouse immunoglobulin G (IgG) and is conjugated with horseradish peroxidase (HRP). The HRP label allows for colorimetric or chemiluminescent detection of target proteins in various immunoassays, such as Western blotting and enzyme-linked immunosorbent assays (ELISAs).
Automatically generated - may contain errors
Lab products found in correlation
Anti rabbit igg hrp antibody, by Cell Signaling Technology (2 mentions)
Anti gapdh antibody, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Hybond p pvdf, by GE Healthcare (1 mentions)
Ecl western blot detection system, by GE Healthcare (1 mentions)
Anti p erk antibody, by Cell Signaling Technology (1 mentions)
Anti p53 antibody, by Cell Signaling Technology (1 mentions)
Anti β actin antibody, by Cell Signaling Technology (1 mentions)
Image lab software, by Bio-Rad (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Chemidoc xrs system, by Bio-Rad (1 mentions)
3 protocols using anti mouse igg hrp antibody
1
GPC3 Expression Analysis in Hepa1-6 Cells
2
Protein Blotting and Detection Workflow
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Hybond-P PVDF and ECL nitrocellulose membranes
were purchased from GE Healthcare (Chicago, IL). The 75 g/m2 uncoated print paper (printing paper) was purchased from Hankuk
Paper (Seoul, Republic of Korea). The antimouse IgG-HRP antibody was
purchased from Cell Signaling Technology (Danvers, MA). The recombinant
human PSMA protein was purchased from Abcam (Cambridge, UK). Recombinant
human TNF-α protein was obtained from Sigma-Aldrich (St. Louis,
MO). Anti-PSMA antibody-HRP was obtained from Lifespan Biosciences
(Washington, WA). Anti-TNF-α antibody-HRP was purchased from
Abcam. The ECL western blot detection system was purchased from GE
Healthcare. The piezo printhead printer (XP-310) was obtained from
Epson (Suwa, Japan).
were purchased from GE Healthcare (Chicago, IL). The 75 g/m2 uncoated print paper (printing paper) was purchased from Hankuk
Paper (Seoul, Republic of Korea). The antimouse IgG-HRP antibody was
purchased from Cell Signaling Technology (Danvers, MA). The recombinant
human PSMA protein was purchased from Abcam (Cambridge, UK). Recombinant
human TNF-α protein was obtained from Sigma-Aldrich (St. Louis,
MO). Anti-PSMA antibody-HRP was obtained from Lifespan Biosciences
(Washington, WA). Anti-TNF-α antibody-HRP was purchased from
Abcam. The ECL western blot detection system was purchased from GE
Healthcare. The piezo printhead printer (XP-310) was obtained from
Epson (Suwa, Japan).
3
Protein Expression Analysis in Cardiomyocytes
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Total proteins were extracted from H9c2 cells and primary cardiomyocytes using RIPA Lysis Buffer (Catalog No. P0013C, Beyotime Biotechnology) and quantified using BCA Protein Assay kit (Catalog No. P0012S, Beyotime Biotechnology). Western blotting was conducted as previously described.21 (link) Following antibodies were used: anti-Cyclin D1 antibody (#2922), anti-p53 antibody (#2524), anti-t-MEK antibody (#4694), anti-p-MEK antibody (#3958), anti-t-ERK antibody (#4695), anti-p-ERK antibody (#4370), anti-β-actin antibody (#4970), anti-rabbit IgG (HRP) antibody (#7074), anti-mouse IgG (HRP) antibody (#7076, Cell Signaling Technology, Beverly, MA, USA), anti-p21 antibody (ab109199), anti-PARP antibody (ab227244), anti-cleaved-PARP antibody (ab32064), anti-Pro-Caspase 3 antibody (ab4051), and anti-Cleaved-Caspase 3 antibody (ab49822, Abcam Biotechnology, Cambridge, MA, USA). Signals of proteins were captured using Bio-Rad ChemiDoc™ XRS system (Bio-Rad Laboratories, Hercules, CA, USA). Intensities of bands were calculated using Image Lab™ software (Bio-Rad Laboratories).
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