Application ver 3

Manufactured by Leica

Leica Application ver.3.2 is a software program designed to operate and control various Leica laboratory equipment. The software provides a user interface for configuring, monitoring, and managing the operation of compatible Leica instruments.

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Lab products found in correlation

Axioscope 2, by Zeiss (2 mentions) Dm1000, by Leica (2 mentions)

2 protocols using application ver 3

Karyotyping and Chromosomal Analysis of Rodents

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Eighteen specimens belonging to seven species were karyotyped. Mitotic chromosome suspensions were made in the field from short-term culture of bone marrow and/or spleen after colchicine treatment in vivo as described previously^{44 (link)}. For U. aff. soricipes, a chromosome suspension from primary fibroblast culture was prepared following the standard technique⁴⁵. Air-dried chromosome spreads of all the specimens were conventionally stained with 2% Giemsa for 1–2 min and sequentially subjected to differential staining.
The standard trypsin/Giemsa staining procedure was carried out for the identification of each chromosome arm by G-bands^{46 (link)}. C-banding was performed by the classic technique^{47 (link)}. NORs were detected by silver nitrate staining^{48 (link)}.
A Leica DFC-295 CCD camera mounted on a DM1000 (Leica) microscope or a Metasystems CCD (Zeiss) camera mounted on an AxioScope 2 (Zeiss) microscope was employed to capture images using MetaSystems Ikaros ver.5.3 and Leica Application ver.3.2 software packages, respectively.

Pavlova S.V., Lebedev V.S., Yakushov V.D., Zhu Y., Fang Y., Sun Y.H, & Sheftel B.I. (2021). High diversity of small insectivorous mammals on Qinghai–Tibet Plateau and first description of karyotype for four endemics of China. Scientific Reports, 11, 24496.

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Karyotyping of Small Mammal Species

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Eighteen specimens belonging to seven species were karyotyped. Mitotic chromosome suspensions were made in the eld from short-term culture of bone marrow and/or spleen after colchicine treatment in vivo as described previously 44 . For U. aff. soricipes, a chromosome suspension from primary broblast culture was prepared following the standard technique 45 . Air-dried chromosome spreads of all the specimens were conventionally stained with 2% Giemsa for 1-2 minutes and sequentially subjected to differential staining.
The standard trypsin/Giemsa staining procedure was carried out for the identi cation of each chromosome arm by G-bands 46 . C-banding was performed by the classic technique 47 . NORs were detected by silver nitrate staining 48 .
A Leica DFC-295 CCD camera mounted on a DM1000 (Leica) microscope or a Metasystems CCD (Zeiss) camera mounted on an AxioScope 2 (Zeiss) microscope was employed to capture images using MetaSystems Ikaros ver.5.3 and Leica Application ver.3.2 software packages, respectively.

Pavlova S.V., Lebedev V.S., Yakushov V.D., Zhu Y., Fang Y., Sun Y., & Sheftel B.I. (2021). High Diversity of Small Insectivorous Mammals on Qinghai-Tibet Plateau and First Description of Karyotype for four Endemics of China.

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