Microparticles generated with
a 150 μm sieve were suspended
at 5–10 mg/mL concentrations in a 5% surfactant (polidocanol)
solution. Particle sizing was performed with a Horiba LA-960 Particle
Size Analyzer (Kyoto, Japan) at the Materials Science and Engineering
Department at the University of Washington. Deionized water with a
real refractive index of 1.33 was used as the dispersion medium; electrospun
fibers loaded with 20 wt % doxycycline and 80 wt % phenyl benzoate
were sized with real refractive indices of 1.54 and 1.58, respectively.
Circulation and agitation modes were set to 1, the system was debubbled,
aligned, and blanked. Sample suspensions were added until transmittance
was reduced by approximately 5%, and then measurements were taken
in n = 5 replicates. Particle diameter was calculated
based on a Mie Theory scattering algorithm and reported as the volumetric
mean, D[4,3], and span is calculated according to the following equation: where Dv,0.9 represents the diameter
where 90% of the volumetric distribution
lies below, the Dv,0.1 where 10% of the volumetric distribution lies below, and the Dv,0.5 represents the median
diameter in which half of the distribution lies above and half lies
below.
a 150 μm sieve were suspended
at 5–10 mg/mL concentrations in a 5% surfactant (polidocanol)
solution. Particle sizing was performed with a Horiba LA-960 Particle
Size Analyzer (Kyoto, Japan) at the Materials Science and Engineering
Department at the University of Washington. Deionized water with a
real refractive index of 1.33 was used as the dispersion medium; electrospun
fibers loaded with 20 wt % doxycycline and 80 wt % phenyl benzoate
were sized with real refractive indices of 1.54 and 1.58, respectively.
Circulation and agitation modes were set to 1, the system was debubbled,
aligned, and blanked. Sample suspensions were added until transmittance
was reduced by approximately 5%, and then measurements were taken
in n = 5 replicates. Particle diameter was calculated
based on a Mie Theory scattering algorithm and reported as the volumetric
mean, D[4,3], and span is calculated according to the following equation: where Dv,0.9 represents the diameter
where 90% of the volumetric distribution
lies below, the Dv,0.1 where 10% of the volumetric distribution lies below, and the Dv,0.5 represents the median
diameter in which half of the distribution lies above and half lies
below.