Irdye 800cw or 680lt conjugated secondary antibodies

For Reelin protein levels, protein extracts were prepared from cortical tissue extracted from WT and KO P2 mice. For other proteins, extracts were prepared from somatosensory cortical tissue, dissected from 400-μm-thick brain sections of WT and KO adult mice. Western blotting was performed using 10–12% SDS polyacrylamide precast gels (BIO-RAD Laboratories) with 15–30 μg of protein extracts loaded per lane. Protein transfer on nitrocellulose membrane was done by using the Trans-Blot Turbo Transfer System (BIO-RAD Laboratories). Nitrocellulose filters were incubated with primary antibodies raised against Reelin (G10, 1:1000; GeneTex), NeuN (A60, 1:1000; Chemicon), GFAP (G-A-5, 1:500; Sigma-Aldrich), GAD65 (GAD-6, 1:1000; abcam), GAD67 (1G10.2, 1:1000; Merck Millipore), Synapsin I (S193, 1:2000; Sigma-Aldrich), and GAPDH (6C5, 1:500; Merck Millipore) overnight at 4°C, followed by IRDye 800CW- or 680LT-conjugated secondary antibodies (1:1500; LI-COR). To visualize bands, Odyssey Fc Imager was used. Densitometric analysis was performed using Image Studio Software (LI-COR), and each band was normalized to the GAPDH signal in each lane and expressed as percentage.