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7900ht analyser

Manufactured by Thermo Fisher Scientific
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The 7900HT analyser is a real-time PCR (polymerase chain reaction) system designed for accurate and sensitive gene expression analysis. It provides quantitative measurement of target gene expression levels through fluorescence-based detection.

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Lab products found in correlation

Sequence detection software version 2, by Thermo Fisher Scientific (1 mentions) Random hexamer primer, by Promega (1 mentions) M mlv reverse transcriptase, by Promega (1 mentions)

2 protocols using 7900ht analyser

1

APOE Genotyping from Whole Blood

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DNA was extracted from whole blood samples; two TaqMan assays (Rs429358 and Rs7412, Assay-On-Demand, Applied Biosystems) were used and run on a 7900HT analyser (Applied Biosystems) and genotypes indicated by the Sequence Detection Software version 2.0 (Applied Biosystems). APOE was modelled as those with and without ε4 allele(s).
Singh-Manoux A., Oumarou-Ibrahim A., Machado-Fragua M.D., Dumurgier J., Brunner E.J., Kivimaki M., Fayosse A, & Sabia S. (2022). Association between kidney function and incidence of dementia: 10-year follow-up of the Whitehall II cohort study. Age and Ageing, 51(1), afab259.
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2

Total RNA Extraction and RT-qPCR Analysis

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Total RNA was extracted as before and reverse transcribed using MMLV Reverse Transcriptase (Promega) with random hexamer primers (Promega) according to the manufacturer's instructions. All RT-qPCR assays were performed with exon-spanning TaqMan RNA expression assays (Invitrogen) using Universal Master Mix II (Invitrogen two) on a 7900HT analyser (Applied Biosystems). Treated samples were normalized to controls with the ΔΔCt formula using 18S rRNA as an endogenous control.
Hopkins T.G., Mura M., Al-Ashtal H.A., Lahr R.M., Abd-Latip N., Sweeney K., Lu H., Weir J., El-Bahrawy M., Steel J.H., Ghaem-Maghami S., Aboagye E.O., Berman A.J, & Blagden S.P. (2015). The RNA-binding protein LARP1 is a post-transcriptional regulator of survival and tumorigenesis in ovarian cancer. Nucleic Acids Research, 44(3), 1227-1246.
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