Conventional formulation of metribuzin (Sencor® 480), technical metribuzin (95% purity), and 14C-metribuzin (98% purity and 2.3 Bq mg−1 of specific activity, American Radiolabeled Chemicals, Inc., St. Louis, MO, USA) and Scintillation solution (Ultima Gold, PerkinElmer, Waltham, MA, USA) were used in the studies. In nanoparticle preparation, caprylic/capric triglyceride (Myritol 318) was purchased from Basf (Basf Co. Ltd., São Paulo, SP, Brazil), poly-ε-caprolactone (PCL) (Mn∼80,000 Da), polysorbate 80 (Mn∼1310 Da), and sorbitane monostearate (Mw = 430.63 g mol−1) were purchased from Sigma Aldrich (Sigma-Aldrich, Chem. Co., St. Louis, MO, USA). Soil samples were collected in Paraná and São Paulo State and organic residues were collected in agricultural areas.
Sorbitane monostearate
Manufactured by Merck Group
Sourced in Brazil, United Kingdom
Sorbitane monostearate is a non-ionic surfactant and emulsifier used in various pharmaceutical and cosmetic applications. It is a derivative of sorbitol and stearic acid. Sorbitane monostearate acts as a wetting agent, solubilizer, and stabilizer in formulations.
Automatically generated - may contain errors
Lab products found in correlation
Polysorbate 80, by Merck Group (2 mentions)
Ultima gold, by PerkinElmer (2 mentions)
14c metribuzin, by American Radiolabeled Chemicals (2 mentions)
Poly ε caprolactone pcl, by Merck Group (1 mentions)
Sorbitan stearate, by Merck Group (1 mentions)
Cholesterol, by Thermo Fisher Scientific (1 mentions)
Chloroform, by Merck Group (1 mentions)
Span 60, by Merck Group (1 mentions)
Polycaprolactone, by Merck Group (1 mentions)
3 protocols using sorbitane monostearate
1
Radioactive Metribuzin Nanoparticle Formulation
2
Formulation and Evaluation of Lipid-Based Delivery
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Pharmaceutical grade PG (99.90%) was kindly supplied from Pfizer Inc., U.S.A., Cholesterol (95% stabilized) was purchased from Acros organics, U.K. Span 60, sorbitan stearate, sorbitane monostearate and chloroform were purchased from Sigma-Aldrich, Germany. HydroxyPropylemethylcellulose (HPMC) was kindly supplied from Cairo Pharmaceuticals Co., Egypt. Carbopol 934 was kindly provided by El-Nil Co. Pharmaceuticals & Chemical Industries, Cairo, Egypt. Monopotassium dihydrogen phosphate and sodium phosphate dibasic anhydrous were bought from Adwic, Al Nasr Pharmaceutical Chemicals.co. Egypt. Cellophane membrane; Spectra/por dialysis membrane 12000–14000 Mwt cut off was used.
3
Metribuzin Formulation and Enzyme Assays
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Polycaprolactone (Mn ∼
80,000 Da), sorbitane monostearate (Mw: 430.63 g mol–1), and polysorbate 80 (Mn ∼ 1310 Da) were purchased from Sigma-Aldrich
(Sigma-Aldrich, Chem. Co.) and caprylic/capric triglyceride (Myritol
318) from Basf Co. Ltd, Brazil. Technical-grade metribuzin (95%) and
the conventional formulation (Sencor 500 CG), 14C-metribuzin
(95%) with a specific activity of 2.3 Bq mg–1 (American
Radiolabeled Chemicals, Inc., St. Louis, MO), and scintillation solution
(Ultima Gold, PerkinElmer, Waltham, MA) were also used. Acetone ACS
(>99.5%) and high-performance liquid chromatography (HPLC)-grade
methanol
were purchased from Dinâmica Co. Ltd, Brazil.
The substrates
(4-methylumbelliferone-phosphate (Mub-P), 4-methylumbelliferone-glucopyranoside
(Mub-C), and 4-methylumbelliferone-sulfate (Mub-S)) and fluorophore
(4-methylumbelliferone, Mub) for the enzyme assays were purchased
from Sigma-Aldrich. Other reagents (analytical grade or better) were
purchased from local suppliers.
80,000 Da), sorbitane monostearate (Mw: 430.63 g mol–1), and polysorbate 80 (Mn ∼ 1310 Da) were purchased from Sigma-Aldrich
(Sigma-Aldrich, Chem. Co.) and caprylic/capric triglyceride (Myritol
318) from Basf Co. Ltd, Brazil. Technical-grade metribuzin (95%) and
the conventional formulation (Sencor 500 CG), 14C-metribuzin
(95%) with a specific activity of 2.3 Bq mg–1 (American
Radiolabeled Chemicals, Inc., St. Louis, MO), and scintillation solution
(Ultima Gold, PerkinElmer, Waltham, MA) were also used. Acetone ACS
(>99.5%) and high-performance liquid chromatography (HPLC)-grade
methanol
were purchased from Dinâmica Co. Ltd, Brazil.
The substrates
(4-methylumbelliferone-phosphate (Mub-P), 4-methylumbelliferone-glucopyranoside
(Mub-C), and 4-methylumbelliferone-sulfate (Mub-S)) and fluorophore
(4-methylumbelliferone, Mub) for the enzyme assays were purchased
from Sigma-Aldrich. Other reagents (analytical grade or better) were
purchased from local suppliers.
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