Blood tests were performed at three experimental time-points: before (baseline, BL), at the end of the hemorrhage (T0H), and at T6H on the 46 animals. Blood samples were collected from the arterial catheter to measure arterial blood gases (Rapidlab 348 EX, Siemens HealthCare), glycemia (Verio, OneTouch), and lactatemia (THE EDGE, ApexBio). The PaO2/FiO2 ratio was the calculated by dividing the arterial oxygen level by the fraction of inspired oxygen measured on the ventilation mask. Alveolar-arterial gradient is a measure by the difference between the alveolar oxygen concentration (calculated from the blood gas data) and the arterial oxygen concentration (measured from the blood gases).
Rapidlab 348ex
Manufactured by Siemens
Sourced in Germany, United Kingdom
The RAPIDLab® 348EX is a lab equipment product from Siemens. It is an automated blood gas and electrolyte analyzer designed for critical care settings.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using rapidlab 348ex
1
Blood gas analysis in hemorrhage
2
Multimodal Monitoring of Media Preparation
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Media preparation processes of the first study were monitored with a pH‐probe (EasyFerm®, Hamilton, USA) and two different spectroscopic monitoring methods. Fluorescence was measured with a 2D FL probe (BioView®, DELTA, Denmark) with an excitation range from 290 to 550 nm and an emission range from 310 to 590 nm. The measurement increment for the corresponding excitation‐emission matrix (EEM) was set to 20 nm with a gain of 1500 and a sampling time of 1 min per EEM. MIR measurements were conducted by a ReactIR™ iC10 probe (Mettler Toledo, Switzerland) with a silver halide glass fiber. The FTIR instrument consisted of an ATR diamond tipped probe. The measurement range was set between 650 and 2000 cm−1 at a resolution of 5 cm−1. The sampling time was 1 min per measurement. The used mercury cadmium telluride (MCT) detector was cooled with liquid nitrogen. Data analysis was only performed in the fingerprinting region between 800 and 1500 cm−1.
The media preparations of the second study were monitored by a pH and 2D FL probe as described above and additionally with an oxidation reduction potential (ORP) probe (EasyFerm® Plus ORP Arc 120, Hamilton, USA) and a dissolved oxygen (DO) probe (VisiFerm® DO 225, Hamilton, USA). The pCO2 was measured offline at the end of the media preparation with a blood gas analyzer (RAPIDLab® 348EX, Siemens, Germany).
The media preparations of the second study were monitored by a pH and 2D FL probe as described above and additionally with an oxidation reduction potential (ORP) probe (EasyFerm® Plus ORP Arc 120, Hamilton, USA) and a dissolved oxygen (DO) probe (VisiFerm® DO 225, Hamilton, USA). The pCO2 was measured offline at the end of the media preparation with a blood gas analyzer (RAPIDLab® 348EX, Siemens, Germany).
3
Diagnosing Dengue-associated Acute Liver Failure
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Dengue-associated acute liver failure (PALF) was diagnosed according to the 2017 EASL Clinical Practical Guidelines.[18 (link)] The vasoactive-inotropic score (VIS) was used to assess hemodynamic support.[19 (link)] Serum lactate levels were measured using a machine-Alinity® ci-series (Abbott), and the normal range of serum lactate: 1.8–2.7 mmol/L. Additionally, serum bicarbonate was analyzed by arterial blood gas analysis using the fully automated system-RapidLab®348EX (Siemens Healthcare Diagnostics Inc., UK) with a normal bicarbonate range: 22 to 26 mEq/L.
4
Comprehensive Biomarker Assessment
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Complete blood count (Sysmex® x5 500; Kobe, Japan), kidney and liver function tests, serum electrolytes, C-reactive protein (COBAS INTEGRA® 400; Hoffman-La Roche Ltd., Basel, Switzerland), arterial blood gas analysis (RAPIDLab® 348EX; Siemens, Munich, Germany).
5
Liver Preservation Protocol Optimization
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In the control (CT) group, the liver without CPA was reperfused immediately after procurement on an IPRL (n = 6). In the other groups, all animals were subjected to 30 min of CPA, and the livers were removed thereafter. In the CS group, livers were stored in UW solution at 4 °C for 3 h (n = 6). The HMP group was further divided into four groups. Livers were treated with 3 h of HMP with UW-MPS® without pH adjustment in the MP group (n = 6), while in the MP-pH groups, livers were subjected to 3 h of HMP with UW-MPS® with pH adjustment to 7.6, 7.8, and 8.0 at 7–10 °C (MP-pH 7.6, MP-pH 7.8, and MP-pH 8.0 groups, respectively) (each n = 6). The pH during HMP was monitored using a pH meter (LAQUAact, HORIBA, Kyoto, Japan) throughout the HMP period in all groups. The values of pO2 and pCO2 were measured using a RAPIDLab 348EX (SIEMENS, Munich, Germany).
6
Comprehensive Blood Gas and CBC Analysis
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Blood gases were determined from whole blood using an autoanalyzer (Rapid Lab 348EX; Siemens, Berlin, Germany). Blood gas analysis included pH, pCO2, pO2, Na+, K+, Ca++,
Cl−, HCO3, base excess (BE), CO2 concentration (ctCO2), Ca++ (7.4) and O2SAT. CBC was determined using an automated hematology analyzer (KX-21; SYSMEX, Kobe,
Japan). CBC analyses included WBC count, RBC count, hemoglobin (HGB), HCT, MCV, MCH, MCHC, platelets (PLT), red blood cell distribution width (RDW), PLT cell distribution width (PDW) and MPV.
Cl−, HCO3, base excess (BE), CO2 concentration (ctCO2), Ca++ (7.4) and O2SAT. CBC was determined using an automated hematology analyzer (KX-21; SYSMEX, Kobe,
Japan). CBC analyses included WBC count, RBC count, hemoglobin (HGB), HCT, MCV, MCH, MCHC, platelets (PLT), red blood cell distribution width (RDW), PLT cell distribution width (PDW) and MPV.
7
Comprehensive Metabolic Profiling of Expedition
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Thyroid stimulating hormone (TSH), unbound thyroxine (fT4) and total T3 (tT3) were measured from gel-separated serum using Abbott ® Architect analyzer (Abbott, Maidenhead, UK) according to manufacturer's instructions.
Insulin-like growth factor 1 (IGF-1), ferritin, insulin and Cpeptide were determined from gelseparated serum using Roche ® Cobas e411 analyzer (Roche Diagnostics, Welwyn Garden City, UK) according to manufacturer's instructions. Creatinine, albumin, transferrin, calcium, zinc, iron and magnesium were determined from gelseparated serum and glucose and lactate from plasma containing fluoride oxalate using commercial kits (Alpha Laboratories, Eastleigh, UK) adapted for use on a Cobas Fara centrifugal analyzer (Roche, UK). Leptin was measured by ELISA (Quantikine, USA). Urea was determined from gelseparated serum using a commercial kit (Randox laboratories, UK) adapted for use on a Cobas Fara centrifugal analyzer. Plasma pH was detected in using a blood gas analyzer (Siemens RapidLab 348EX, Camberley, UK). Homeostatic modelling assessment (HOMA) for beta cell function (HOMA-B) insulin sensitivity (HOMA-S) and insulin resistance (HOMA-IR) were calculated according to the methods of Levy et al. (23) .
Additional data including resting energy expenditure and substrate utilization from direct calorimetry pre-and post-expedition are being published elsewhere.
Insulin-like growth factor 1 (IGF-1), ferritin, insulin and Cpeptide were determined from gelseparated serum using Roche ® Cobas e411 analyzer (Roche Diagnostics, Welwyn Garden City, UK) according to manufacturer's instructions. Creatinine, albumin, transferrin, calcium, zinc, iron and magnesium were determined from gelseparated serum and glucose and lactate from plasma containing fluoride oxalate using commercial kits (Alpha Laboratories, Eastleigh, UK) adapted for use on a Cobas Fara centrifugal analyzer (Roche, UK). Leptin was measured by ELISA (Quantikine, USA). Urea was determined from gelseparated serum using a commercial kit (Randox laboratories, UK) adapted for use on a Cobas Fara centrifugal analyzer. Plasma pH was detected in using a blood gas analyzer (Siemens RapidLab 348EX, Camberley, UK). Homeostatic modelling assessment (HOMA) for beta cell function (HOMA-B) insulin sensitivity (HOMA-S) and insulin resistance (HOMA-IR) were calculated according to the methods of Levy et al. (23) .
Additional data including resting energy expenditure and substrate utilization from direct calorimetry pre-and post-expedition are being published elsewhere.
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