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Hiscribe t7 synthesis kit

Manufactured by New England Biolabs
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The HiScribe T7 synthesis kit is a tool designed for in vitro transcription of RNA from DNA templates using the T7 RNA polymerase. The kit provides the necessary components, including T7 RNA polymerase, reaction buffer, and ribonucleotides, to facilitate the synthesis of RNA molecules.

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Lab products found in correlation

Purelink rna mini kit, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

HiScribe T7 High Yield RNA Synthesis Kit (372 citations) HiScribe T7 Quick High Yield RNA Synthesis Kit (224 citations) HiScribe T7 RNA Synthesis Kit (16 citations) HiScribe T7 Kit (15 citations) NEB HiScribe T7 High Yield RNA Synthesis Kit (4 citations) HiScribe T7 Yield RNA Synthesis Kit (2 citations) HiScribe T7 Fast High Yield RNA Synthesis Kit (2 citations) HiScribe T7 High Yield RNA Synthesis Kit Catalog (2 citations) HiScribe T7 rapid and efficient RNA synthesis kit (2 citations)

2 protocols using hiscribe t7 synthesis kit

1

In vitro Transcription for Replicon Assays

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In vitro transcription reactions for replicon assays were performed as described previously (26). Transcription reactions to produce large amounts of RNA for SHAPE analysis were performed with purified linear DNA. 1 μg of linearised DNA was used in a HiScribe T7 synthesis kit (NEB), before DNase treatment and purification using a PureLink RNA mini kit (Thermo Fisher).
Ward J.C., Lasecka-Dykes L., Neil C., Adeyemi O.O., Gold S., McLean-Pell N., Wright C., Herod M.R., Kealy D., Warner E., Jackson T., King D.P., Tuthill T.J., Rowlands D.J, & Stonehouse N.J. (2022). The RNA pseudoknots in foot-and-mouth disease virus are dispensable for genome replication, but essential for the production of infectious virus. PLoS Pathogens, 18(6), e1010589.
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2

In Vitro Transcription for Replicon Assays

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In vitro transcription reactions for replicon assays were performed as described previously (28) .
Transcription reactions to produce large amounts of RNA for SHAPE analysis were performed with purified linear DNA as described above, and 1 μg of linearised DNA was then used in a HiScribe T7 synthesis kit (NEB), before DNase treatment and purification using a PureLink RNA mini kit (Thermo Fisher).
Ward J.C., Lasecka‐Dykes L., Neil C., Adeyemi O.O., Gold S., McLean N., Wright C.F., Herod M.R., Kealy D., Warner E., King D.P., Tuthill T.J., Rowlands D.J., & Stonehouse N.J. (2020). The RNA pseudoknots in foot-and-mouth disease virus are dispensable for genome replication but essential for the production of infectious virus.
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