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Hiscribe t7 synthesis kit

Manufactured by New England Biolabs

The HiScribe T7 synthesis kit is a tool designed for in vitro transcription of RNA from DNA templates using the T7 RNA polymerase. The kit provides the necessary components, including T7 RNA polymerase, reaction buffer, and ribonucleotides, to facilitate the synthesis of RNA molecules.

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2 protocols using hiscribe t7 synthesis kit

1

In vitro Transcription for Replicon Assays

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In vitro transcription reactions for replicon assays were performed as described previously (26). Transcription reactions to produce large amounts of RNA for SHAPE analysis were performed with purified linear DNA. 1 μg of linearised DNA was used in a HiScribe T7 synthesis kit (NEB), before DNase treatment and purification using a PureLink RNA mini kit (Thermo Fisher).
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2

In Vitro Transcription for Replicon Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
In vitro transcription reactions for replicon assays were performed as described previously (28) .
Transcription reactions to produce large amounts of RNA for SHAPE analysis were performed with purified linear DNA as described above, and 1 μg of linearised DNA was then used in a HiScribe T7 synthesis kit (NEB), before DNase treatment and purification using a PureLink RNA mini kit (Thermo Fisher).
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