Hiscribe t7 synthesis kit
The HiScribe T7 synthesis kit is a tool designed for in vitro transcription of RNA from DNA templates using the T7 RNA polymerase. The kit provides the necessary components, including T7 RNA polymerase, reaction buffer, and ribonucleotides, to facilitate the synthesis of RNA molecules.
Lab products found in correlation
2 protocols using hiscribe t7 synthesis kit
In vitro Transcription for Replicon Assays
In Vitro Transcription for Replicon Assays
Transcription reactions to produce large amounts of RNA for SHAPE analysis were performed with purified linear DNA as described above, and 1 μg of linearised DNA was then used in a HiScribe T7 synthesis kit (NEB), before DNase treatment and purification using a PureLink RNA mini kit (Thermo Fisher).
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