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Hexadecyltrimethylammonium chloride

Manufactured by Merck Group
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Sourced in Germany, United States, Belgium

Hexadecyltrimethylammonium chloride is a cationic surfactant used in various laboratory applications. It functions as a detergent, wetting agent, and emulsifier. The product is commonly used in biochemical and analytical procedures, but its specific applications may vary depending on the needs of the laboratory.

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Lab products found in correlation

Sodium borohydride, by Merck Group (11 mentions) Hexadecyltrimethylammonium bromide, by Merck Group (9 mentions) L ascorbic acid, by Merck Group (6 mentions) Hydrochloric acid (hcl), by Merck Group (5 mentions) Silver nitrate, by Merck Group (4 mentions) Ascorbic acid, by Merck Group (3 mentions) Trypsin edta, by Thermo Fisher Scientific (3 mentions) Ethanol, by Xilong (3 mentions) Ascorbic acid, by Xilong (3 mentions) Potassium iodide, by Merck Group (2 mentions) Gold 3 chloride trihydrate, by Merck Group (2 mentions) Potassium bromide, by Merck Group (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Benzyldimethylhexadecylammonium chloride, by Merck Group (2 mentions) Citric acid, by Merck Group (2 mentions) Polyvinylpyrrolidone mw 55 000, by Merck Group (2 mentions) 1 3 3 1 3 3 hexamethyl 2 2 indotricarbocyanine iodide, by Merck Group (2 mentions) Sodium citrate dihydrate, by BD (2 mentions) Methoxy polyethylene glycol thiol mw 5000 mpeg sh, by Nanocs (2 mentions) Synergy ultrapure water, by Merck Group (2 mentions)

20 protocols using hexadecyltrimethylammonium chloride

1

Synthesis of Gold Nanoparticles

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All the reactants were purchased from Sigma-Aldrich and used without further purification: hexadecyltrimethylammonium bromide (CTAB, ≥99%), hexadecyltrimethylammonium chloride (CTAC, 25 wt% in H2O), hydrogen tetrachloroaurate trihydrate (HAuCl4·3H2O, ≥99.9%), silver nitrate (AgNO3, ≥99%), l-ascorbic acid (AA, ≥99%), sodium borohydride (NaBH4, 99%). Water purified by reverse osmosis with a resistivity (>15 MΩ cm) was used in all experiments.
Lyu J., Geertsen V., Hamon C, & Constantin D. (2020). Determining the morphology and concentration of core–shell Au/Ag nanoparticles. Nanoscale Advances, 2(10), 4522-4528.
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2

Synthesis of Metal Nanoparticles

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Gold(III) chloride trihydrate (HAuCl4∙3H2O, 99.9%), trisodium citrate, sodium borohydride (NaBH4, 99%), hexadecyltrimethyl ammonium chloride (CTAC, 25 wt% solution in water), hexadecyltrimethyl ammonium bromide (CTAB, 99%), potassium bromide (KBr, 99%), potassium iodide (KI, 99%), L-ascorbic acid (99%), lead(II) chloride (PbCl2, 99.9%), and sodium thiosulfate (Na2S2O3, 99%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 2-Mercapto ethanol was obtained from Merck (Darmstadt, Germany). Deionized water was used throughout whole experiments.
Park E.J, & Ha T.H. (2024). Pb2+ Ion Sensors Employing Gold Etching Process: Comparative Investigation on Au Nanorods and Au Nanotriangles. Sensors (Basel, Switzerland), 24(2), 497.
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3

Synthesis and Purification of Gold Nanoparticles

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All utilized chemicals were obtained commercially and used without further purification. Tetrachloroauric(III) acid trihydrate (HAuCl4 ≥ 99.5%) and L(+)-ascorbic acid (AA, ≥99%) were purchased from Carl Roth GmbH & Co KG (Karlsruhe, Germany). Hexadecyltrimethylammonium chloride (CTAC, >99%) implemented in synthesis procedure was obtained from Molekula Group GmbH (Munich, Germany). Hexadecyltrimethylammonium chloride (CTAC, 25 wt% in water) implemented in purification procedure, sodium borohydride (NaBH4, 99.99%), and sodium idode (NaI, ≥99.5%) were purchased from Sigma-Aldrich (Darmstadt, Germany). The solutions were prepared using Millipore water. Prior to use, all glassware and magnetic stirrers were washed with aqua regia (caution: aqua regia is highly toxic and corrosive) and rinsed thoroughly with Millipore water.
Podlesnaia E., Csáki A, & Fritzsche W. (2021). Time Optimization of Seed-Mediated Gold Nanotriangle Synthesis Based on Kinetic Studies. Nanomaterials, 11(4), 1049.
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4

Synthesis of Luminescent Lanthanide Nanoparticles

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Yttrium(iii) acetate hydrate (99.9%, ALFA Aesar), ytterbium(iii) acetate hydrate (99.9%, ALFA Aesar), thulium(iii) acetate hydrate (99.9%, ALFA Aesar), oleic acid (90%, Sigma-Aldrich), 1-octadecene (90%, ACROS), ammonium fluoride (98%, Merck), sodium hydroxide (98%, Merck), cyclohexane (99%, JT Baker), tetraethyl orthosilicate (99.0%, Sigma-Aldrich), methanol (99.9%, Fisher), hexadecyltrimethylammonium chloride (≥98.0%, Sigma-Aldrich), hydrochloric acid (37%, Sigma-Aldrich), polyethylenimine (branched, Mw 70 000, 30% w/v aq., Polysciences), phosphate buffered saline (powder, pH 7.4, Sigma-Aldrich), trypsin–EDTA (0.5%, no phenol red, Thermo Fisher Scientific), and ethanol (99.5%, Sigma-Aldrich) were used in the experiments. All chemicals were of analytical grade and were used as received without further purification.
Wang J.H., Chen H.Y., Chuang C.C, & Chen J.C. (2020). Study of near-infrared light-induced excitation of upconversion nanoparticles as a vector for non-viral DNA delivery. RSC Advances, 10(67), 41013-41021.
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5

Photodynamic Therapy Nanoparticle Synthesis

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Bovine serum albumin (BSA), Chloroauric acid (HAuCl4·3H2O), Hexadecyltrimethylammonium bromide (CTAB) (C19H42BrN), Hexadecyltrimethylammonium chloride (CTAC) (C19H42ClN), ascorbic acid (AA), Sodium borohydride (NaBH4), Silver nitrate (AgNO3), sodium citrate (Na3C6H5O7·3H2O), phosphate buffer saline (PBS), N-(3-Dimethylaminopropyl)-N′-ethyl carbodiimide hydrochloride (EDC), N-Hydroxysuccinimide (NHS), Sodium chloride (NaCl), and agarose were all purchased from Sigma-Aldrich (Germany). Holosens®, octachloride octakis [N (2-hydroxyethyl) -N,N, -(dimethylammoniomethyl)] zinc (II) phthalocyanine (ZnPc) was chosen as a photodynamic dye and obtained from Organic Intermediates and Dyes Institute (Russia). Deionized (DI) water with specific resistivity, higher than 18.2 MΩm from a Milli-Q Integral 3 water purification system (Millipore, MA, USA), was used to make all solutions.
Maksimova E.A., Barmin R.A., Rudakovskaya P.G., Sindeeva O.A., Prikhozhdenko E.S., Yashchenok A.M., Khlebtsov B.N., Solovev A.A., Huang G., Mei Y., Kanti Dey K, & Gorin D.A. (2021). Air-Filled Microbubbles Based on Albumin Functionalized with Gold Nanocages and Zinc Phthalocyanine for Multimodal Imaging. Micromachines, 12(10), 1161.
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6

Synthesis of Luminescent Nanoparticles

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Ir(acac)3 (97%), Pt(acac)2 (97%), YCl3 (99.99%), hexadecyltrimethylammonium chloride (> 98%), trioctylphosphine oxide (90%), and oleylamine (98%) were purchased from Sigma-Aldrich. NiCl2 (98%) was purchased from Alfa-Aesar. All reagents were used as received without further purification.
Kim T., Lee Y., Hong Y., Lee K, & Baik H. (2023). Three-dimensional reconstruction of Y-IrNi rhombic dodecahedron nanoframe by STEM/EDS tomography. Applied Microscopy, 53, 9.
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7

Synthesis of Gold Nanoparticles

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Hexadecyltrimethylammonium bromide (CTAB, 99%), hexadecyltrimethylammonium chloride (CTAC, 98%), L-ascorbic acid (99%), tetrachloroauric(III) trihydrate (HAuCl4 · 3H2O, 99.9%), L-glutathione (98%), sodium borohydride (NaBH4, 99%), and potassium iodide (KI, 99.5%) were purchased from Sigma-Aldrich. High-purity deionized water (18.2 MΩ cm−1) was used to prepare aqueous solution.
Choi S., Im S.W., Huh J.H., Kim S., Kim J., Lim Y.C., Kim R.M., Han J.H., Kim H., Sprung M., Lee S.Y., Cha W., Harder R., Lee S., Nam K.T, & Kim H. (2023). Strain and crystallographic identification of the helically concaved gap surfaces of chiral nanoparticles. Nature Communications, 14, 3615.
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8

Synthesis of Metal Nanoparticles

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Hexadecyltrimethylammonium
chloride (CTAC,
25 wt % in water), gold(III) chloride trihydrate (HAuCl4, ≥99%), citric acid (≥99.5%), sodium borohydride (NaBH4), hexadecyltrimethylammonium bromide (CTAB, ≥99%),
silver nitrate (AgNO3, ≥99%), hydrochloric acid
(HCl, 37%), l-ascorbic acid (AA, ≥99%), benzyldimethylhexadecylammonium
chloride (BDAC), and potassium tetrachloropalladate(II) (K2PdCl4, 99.99%) were purchased from Sigma-Aldrich. All
chemicals were used further putification. Milli-Q water (resistivity
18.2 MΩ·cm at 25 °C) was used in all experiments.
Winckelmans N., Altantzis T., Grzelczak M., Sánchez-Iglesias A., Liz-Marzán L.M, & Bals S. (2018). Multimode Electron Tomography as a Tool to Characterize the Internal Structure and Morphology of Gold Nanoparticles. The Journal of Physical Chemistry. C, Nanomaterials and Interfaces, 122(25), 13522-13528.
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9

Synthesis of Rhodium Nanoparticles

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Rhodium (III) chloride hydrate (RhCl3·xH2O, Rh 38.5–45.5%), Ethylene glycol (EG, HOCH2CH2OH, >99%), Poly(vinyl pyrrolidone) (PVP, C2H2N(C6H9NO)nC13H10NS2, average MW = 55 kDa), Potassium bromide (KBr, >99%), Sodium Acetate trihydrate (NaAc; CH3COONa·3H2O, >99%), Hexadecyltrimethylammonium bromide (CTAB, CH3(CH2)15N(Br)(CH3)3, >99%), Hexadecyltrimethylammonium chloride (CTAC, CH3(CH2)15N(Cl)(CH3)3, >99%), and Sodium citrate dihydrate (NaCit; HOC(COONa)(CH2COONa)2·2H2O, 99%) were obtained from Sigma Aldrich, Germany. Hydrochloric acid, Sodium hydroxide, and solvents, including acetone and ethanol, were of analytical grade and were obtained from Sigma Aldrich, Sweden. All chemicals were used without further purification.
Li Y., Saladino G.M., Shaker K., Svenda M., Vogt C., Brodin B., Hertz H.M, & Toprak M.S. (2020). Synthesis, Physicochemical Characterization, and Cytotoxicity Assessment of Rh Nanoparticles with Different Morphologies-as Potential XFCT Nanoprobes. Nanomaterials, 10(11), 2129.
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10

Gold Nanoparticle Synthesis Protocol

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Sodium borohydride (NaBH4), Au chloride solution (HAuCl4) 200 mg/dL in deionized water, ascorbic acid (AA), hexadecyltrimethylammonium chloride (CTAC) solution 25 wt% in water, polyvinylpyrrolidone Mw ~55,000 (PVP), ethanol (EtOH), 1,3,3,1′,3′,3′,-hexamethyl-2,2′-indotricarbocyanine iodide (HITC), phosphate buffer saline (PBS), tris-EDTA buffer solution (TE), tween 20, sodium chloride (NaCl), hydrochloric acid (HCl) were purchased from Sigma-Aldrich. Sodium citrate dihydrate was purchased from BDH. Methoxy polyethylene glycol thiol Mw 5000 (mPEG-SH) was purchased from Nanocs. Magnetic beads were purchased from Life Technologies. All DNA sequences were synthesized by Integrated DNA Technologies (IDT, Coralville, IA). Millipore Synergy ultrapure water (DI) of resistivity = 18.2 MΩ cm was used in all nanoparticle synthesis solutions. Nuclease-free water was used in all experiments relating to RNA and DNA.
Vohra P., Strobbia P., Ngo H.T., Lee W.T, & Vo-Dinh T. (2018). Rapid Nanophotonics Assay for Head and Neck Cancer Diagnosis. Scientific Reports, 8, 11410.
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