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Alexa fluor 647 anti rabbit igg h l

Manufactured by Jackson ImmunoResearch
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Alexa Fluor 647-anti-rabbit IgG (H+L) is a secondary antibody conjugated with Alexa Fluor 647 dye. It is designed to detect and visualize rabbit primary antibodies in various immunoassays and imaging techniques.

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Lab products found in correlation

Pimozide, by Cayman Chemical (2 mentions) Alexa fluor 647 anti phospho stat3 tyr705 clone 4 p stat3, by BD (2 mentions) Alexa fluor 647 anti phospho stat5 tyr694 clone 47 stat5, by BD (2 mentions) Alexa fluor 647 anti phospho p44 42 mapk erk1 2 thr202 tyr204 clone 20a, by BD (2 mentions) Anti phospho akt thr308 clone d25e6, by Cell Signaling Technology (2 mentions) Stat3 inhibitor s3i 201, by Selleck Chemicals (2 mentions) Anti hsp 72, by Enzo Life Sciences (1 mentions) Anti bag3, by Proteintech (1 mentions) Anti tubulin, by Merck Group (1 mentions) Anti rabbit igg, by Merck Group (1 mentions) Anti flag, by Merck Group (1 mentions)

3 protocols using alexa fluor 647 anti rabbit igg h l

1

Phosphorylation Profiling of CAR-T Cells

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Phosphorylated proteins in the CAR-T cells were analyzed by intracellular flow cytometry following coculture with NALM-6 or K562 at an E:T ratio of 1:1. The cells were fixed with 1.6 % formaldehyde, followed by permeabilization by ice-cold methanol. The following antibodies were used: Alexa Fluor 647-anti-phospho-STAT3 (Tyr705) (clone 4/P-Stat3, BD Biosciences), Alexa Fluor 647-anti-phospho-STAT5 (Tyr694) (clone 47/Stat5, BD Biosciences), Alexa Fluor 647-anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (clone 20A, BD Biosciences), and anti-phospho-Akt (Thr308) (clone D25E6, Cell Signaling Technology). Alexa Fluor 647-anti-rabbit IgG (H+L) (Jackson ImmunoResearch) was used as the secondary antibody following the staining with the anti–phospho-Akt antibody. The STAT3 inhibitor S3I-201 (Selleck Chemicals) and the STAT5 inhibitor pimozide (Cayman Chemical) were used at concentrations of 25 μM and 5 μM, respectively.
Kagoya Y., Tanaka S., Guo T., Anczurowski M., Wang C.H., Saso K., Butler M.O., Minden M.D, & Hirano N. (2018). A novel chimeric antigen receptor containing a JAK-STAT signaling domain mediates superior antitumor effects. Nature medicine, 24(3), 352-359.
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2

Antibody Characterization Protocol

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Unless stated otherwise, all reagents were obtained from regular commercial sources as mentioned in the methods descriptions. The following antibodies were used: anti-BAG3 (Proteintech Group, 10599-1-AP), anti-FLAG (Sigma-Aldrich, F1804, St. Louis, MO, USA), anti-HSP72 (Enzo Life Sciences, Farmingdale, NY, USA, ADI-SPA-810), HRP anti-mouse IgG (H+L) (Jackson ImmunoResearch Laboratories, West Grove, PA, USA, 715-035-151), Cy3 anti-mouse IgG (H+L) (Jackson ImmunoResearch Laboratories, 715-165-151), HRP anti-rabbit IgG (H+L) (Jackson ImmunoResearch Laboratories, 711-035-152), Alexa Fluor 647 anti-rabbit IgG (H+L) (Jackson ImmunoResearch Laboratories, 711-605-152), anti-rabbit IgG (Sigma-Aldrich, I5006), anti-tubulin (Sigma-Aldrich, T9026), and anti-YES1 (Sigma-Aldrich, HPA026480).
Hiebel C., Stürner E., Hoffmeister M., Tascher G., Schwarz M., Nagel H., Behrends C., Münch C, & Behl C. (2020). BAG3 Proteomic Signature under Proteostasis Stress. Cells, 9(11), 2416.
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3

Phosphorylation Profiling of CAR-T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phosphorylated proteins in the CAR-T cells were analyzed by intracellular flow cytometry following coculture with NALM-6 or K562 at an E:T ratio of 1:1. The cells were fixed with 1.6 % formaldehyde, followed by permeabilization by ice-cold methanol. The following antibodies were used: Alexa Fluor 647-anti-phospho-STAT3 (Tyr705) (clone 4/P-Stat3, BD Biosciences), Alexa Fluor 647-anti-phospho-STAT5 (Tyr694) (clone 47/Stat5, BD Biosciences), Alexa Fluor 647-anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (clone 20A, BD Biosciences), and anti-phospho-Akt (Thr308) (clone D25E6, Cell Signaling Technology). Alexa Fluor 647-anti-rabbit IgG (H+L) (Jackson ImmunoResearch) was used as the secondary antibody following the staining with the anti–phospho-Akt antibody. The STAT3 inhibitor S3I-201 (Selleck Chemicals) and the STAT5 inhibitor pimozide (Cayman Chemical) were used at concentrations of 25 μM and 5 μM, respectively.
Kagoya Y., Tanaka S., Guo T., Anczurowski M., Wang C.H., Saso K., Butler M.O., Minden M.D, & Hirano N. (2018). A novel chimeric antigen receptor containing a JAK-STAT signaling domain mediates superior antitumor effects. Nature medicine, 24(3), 352-359.
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