Diethylaminoethyl deae sephadex a 25

Iron (II) sulfate heptahydrate (FeSO₄·7H₂O) and ferric chloride (FeCl₃·6H₂O) were obtained from J.T. Baker Chemical Co. (Phillipsburg, NJ, USA). Zinc acetate dihydrate (ZnC₄H₆O₄·2H₂O), sodium acetate (CH3COONa), sinigrin hydrate, sulfatase from Helix pomatia, diethylaminoethyl (DEAE)-sephadex A-25, acetonitrile (HPLC grade), methanol (HPLC grade), and ethanol (HPLC grade) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Desulfoglucoraphanin was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Deionized water (18.2 MΩ·cm resistance) used in the protocols was acquired from a Milli-Q Element water purification system (Millipore, Bedford, MA, USA). Commercial broccoli (Brassica oleracea L. var. italica) seeds Vita^® were obtained from “Rancho Los Molinos” company (Tepoztlan, Mexico).

Nineteen GSL standards, namely glucoiberin (GIB), glucolepidiin (GLP), progoitrin (PRO), epiprogoitrin (EPI), glucoraphanin (GRA), glucoraphenin (GRE), sinigrin (SIN), gluconapin (GNA), sinalbin (SNB), glucomoringin (GMR), glucobarbarin (GBA), glucotropaeolin (GTR), glucobrassicanapin (GBN), glucoerucin (GER), glucobrassicin (GBS), 4-hydroxyglucobrassicin (4HGBS), 4-methoxyglucobrassicin (4MGBS), neoglucobrassicin (NGBS) and gluconasturtiin (GNS), were purchased from Cfm Oskar Co. (Marktredwitz, Germany). Diethylaminoethyl (DEAE)- Sephadex-A25, sodium acetate, HCl, and aryl sulfatase (EC 3.1.6.1, type H-1) from Helix pomatia, were obtained from Sigma-Aldrich (St. Louis, MO, USA). HPLC grade acetonitrile, methanol, and water were obtained from Avantor Performance Materials (Center Valley, PA, USA).