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Plentilox3.7 ef1α mcherry vector

Manufactured by Addgene
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The PLentiLox3.7-EF1α-mCherry vector is a lentiviral expression vector that drives the expression of the mCherry fluorescent protein under the control of the EF1α promoter. The vector is designed for lentiviral-mediated gene delivery and expression.

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Lab products found in correlation

Plentilox3, by Addgene (2 mentions) Peiz hiv zsgreen lentivirus vector, by Addgene (1 mentions) Lentivirus vectors, by System Biosciences (1 mentions)

Close spelling variants of this product

PLentiLox3.7 vector PLentiLox3.7

2 protocols using plentilox3.7 ef1α mcherry vector

1

Lentiviral Constructs for miR-221 and QKI-5

Cited 1 time
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The full-length sequence of miR-221 and the full-length coding region of the QKI-5 mRNA (NM_001301085) were amplified by PCR (Table S1) and cloned into the pEIZ-HIV-ZsGreen lentivirus vector and the pLentiLox3.7-EF1α-mCherry vector, a derivative of pLentiLox3.7 (Addgene: #11795), respectively (5 (link)). The lentivirus vectors encoding for the anti-miR-221 construct (miRZip-221) and a non-targeting pre-miRNA (negative control) were purchased from System Biosciences (USA).
Mukohyama J., Isobe T., Hu Q., Hayashi T., Watanabe T., Maeda M., Yanagi H., Qian X., Yamashita K., Minami H., Mimori K., Sahoo D., Kakeji Y., Suzuki A., Dalerba P, & Shimono Y. (2019). miR-221 targets QKI to enhance the tumorigenic capacity of human colorectal cancer stem cells.. Cancer research, 79(20), 5151-5158.
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2

Lentiviral Overexpression of Anti-miR-93 in Breast Cancer Cells

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The sequence of precursor miR‐93 (mature miR‐93 and its 5′‐ and 3′‐ flanking regions) and the full‐length coding region of the WASF3 mRNA (NM_006646.6 [GenBank]) were amplified by PCR and cloned into the pEIZ‐HIV‐ZsGreen or mCherry lentivirus vector (Addgene: #18121) or the pLentiLox3.7‐EF1α‐mCherry vector, a derivative of pLentiLox3.7 (Addgene: #11795), respectively.18 The lentivirus vectors encoding for the anti‐miR‐93 construct (miRZip‐93) and a nontargeting control (negative control) were purchased from System Biosciences. Lentiviruses were produced as previously described.24 Breast cancer cells were infected with lentivirus constructs at a MOI of 5.
Shibuya N., Kakeji Y, & Shimono Y. (2020). MicroRNA‐93 targets WASF3 and functions as a metastasis suppressor in breast cancer. Cancer Science, 111(6), 2093-2103.
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