Quasi elastic light scattering

Manufactured by Wyatt Technology

Quasi-Elastic Light Scattering (QELS) is a laboratory instrument used to measure the size and distribution of particles in a sample. It operates by analyzing the Doppler shift of laser light scattered by the particles, which is related to their Brownian motion and hydrodynamic size.

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Lab products found in correlation

Astra 6, by Wyatt Technology (1 mentions) Tsk gel g5000pwxl, by Tosoh (1 mentions)

2 protocols using quasi elastic light scattering

Oligomerization of αA-crystallin Variants

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αA-crystallins were subjected to SEC-MALS analysis to ascertain whether the introduction of suppressor mutations affected the quaternary oligomerization of αA-crystallin. Proteins were incubated at 37 °C for 1 hour following which fifty micrograms of the protein in PBS (pH 7.4) was injected into a TSK gel G5000_PWXL (Tosoh Bioscience, King of Prussia, PA) size exclusion column attached to a high-performance liquid chromatography (HPLC) system coupled with Refractive Index Detector (RID), and Quasi-Elastic Light Scattering (Wyatt Technology, Goleta, CA) detectors. The molar mass (M_w) and hydrodynamic radius (R_h) of the proteins were determined from light scattering data using the ASTRA 6.1 software package (Wyatt Technology). All analysis were repeated thrice for statistical significance.

Phadte A.S., Mahalingam S., Santhoshkumar P, & Sharma K.K. (2019). Functional rescue of cataract-causing αA-G98R-crystallin by targeted compensatory suppressor mutations in human αA-crystallin. Biochemistry, 58(40), 4148-4158.

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Hydrodynamic Radius of SiZwi Nanoparticles

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Example 5

The hydrodynamic radius (R_h) of SiZwi, aqueous or organic based preparations, was measured in solution using quasielastic light scattering (Wyatt Technologies) to determine whether during the condensation reaction, interparticle bridging and aggregation had taken place.

SiZwi samples having a concentration of 0.033 mg mL⁻¹were prepared in 0.125 M NaCl and 25 mM Tris buffer pH 9.0. The solution was filtered through 0.02 μm and 0.2μ Anotop™ microfilters before and after adding SiZwi respectively. R_hof SiZwi, prepared from 9.11×10⁻⁴moles of SBS per 1 g SiO₂in aqueous solution was 18.8±2 nm. This indicates that during the condensation reaction, interparticle bridging and aggregation does not take place. The data for other samples are shown in Table 1.

TABLE 1

Hydrodynamic radius of SiZwi prepared in water

or DMSO using different SBS amounts.

Moles of SBS perStandard deviation

1 g SiO₂¹Rh (nm)²Rh (nm)(±nm)

9.11 × 10⁻⁴18.818.32.0

7.288 × 10⁻⁴18.418.62.3

5.466 × 10⁻⁴18.418.72.1

3.64 × 10⁻⁴18.818.62.0

³0³19.5N/A2.5

¹Reaction carried out in double distilled water.

²Reactions carried out in DMSO.

³As received, untreated LUDOX ™ silica colloids.

US10087082B2. Stabilized silica colloid (2018-10-02). None [US]. Inventors: Joseph B. Schlenoff [US].

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