We fixed and permeablized astrocytes with 4% paraformaldehyde and 0.2% Triton‐X100 in phosphate buffered saline (PBS). After blocking in 10% donkey serum, we stained astrocytes with the following primary antibodies: anti‐GFAP (1:1,500. Biolegend, 829401), Ki67 (1:200. Invitrogen MA5‐14520), enhanced green fluorescent protein (EGFP) (1:1,000. Aves Labs GFP‐1020), mCherry (1:600. Clontech 632543), Sox9 (1:2,000. Millipore AB5535), Bromodesoxyuridine (BrdU) (1:500, Novusbio NB500‐169), and fluorescent secondary antibodies (Invitrogen). For BrdU staining, cells were pretreated by 2N hydrochloric acid for 20 min before blocking. After three washes in PBS, we mounted the coverslips with VectorShield with DAPI (Vector Labs H1200) and imaged them using an Evos FL Auto 2 inverted fluorescence microscope (Invitrogen) with ×10, and ×20 lenses and the Imager M2 upright fluorescence microscope (Zeiss) with ×10, ×20, and ×40 lenses. We cropped the images with the FIJI and Photoshop softwares.
Evos fl auto 2 inverted fluorescence microscope
Manufactured by Thermo Fisher Scientific
The Evos FL Auto 2 is an inverted fluorescence microscope designed for high-quality imaging of live cells and fluorescent samples. It features automated functions for focus, exposure, and image capture, allowing for consistent and reproducible results. The microscope is equipped with a range of LED light sources for various fluorophores and provides a user-friendly interface for easy operation.
Automatically generated - may contain errors
Lab products found in correlation
Nb500 169, by Thermo Fisher Scientific (2 mentions)
Imager m2 upright fluorescence microscope, by Zeiss (2 mentions)
Ma5 14520, by Thermo Fisher Scientific (2 mentions)
Fluorescent secondary antibody, by Thermo Fisher Scientific (2 mentions)
Gfp 1020, by Aves Labs (2 mentions)
Vectorshield with dapi, by Vector Laboratories (1 mentions)
Ab5535, by Novus Biologicals (1 mentions)
2 protocols using evos fl auto 2 inverted fluorescence microscope
1
Immunocytochemical Analysis of Astrocytes
2
Immunofluorescence Staining of Astrocytes
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