Pe or apc conjugated streptavidin

Manufactured by BioLegend

Sourced in Germany

Streptavidin is a protein derived from the bacterium Streptomyces avidinii. It has a high affinity for the small molecule biotin. PE- or APC-conjugated streptavidin is a fluorescently labeled version of streptavidin, where the PE (phycoerythrin) or APC (allophycocyanin) fluorescent dye is covalently attached to the streptavidin protein. This allows the streptavidin to be used to detect and visualize biotinylated molecules in various applications, such as flow cytometry and immunoassays.

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Lab products found in correlation

Nonessential amino acid, by Thermo Fisher Scientific (1 mentions) Fixable viability dye efluor 780, by Thermo Fisher Scientific (1 mentions) Zombie aqua fixable viability kit, by BioLegend (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) 2 mercaptoethanol, by Merck Group (1 mentions) Lsrfortessa x 20, by BD (1 mentions) Diva software, by BD (1 mentions) Foxp3 staining kit, by Thermo Fisher Scientific (1 mentions) Attune nxt flow cytometer, by Thermo Fisher Scientific (1 mentions) Gvb buffer, by Complement Technology (1 mentions)

Close spelling variants of this product

Streptavidin-PE (106 citations) Streptavidin-APC (85 citations) PE-conjugated streptavidin (30 citations) APC-conjugated streptavidin (25 citations)

3 protocols using pe or apc conjugated streptavidin

Flow Cytometric Analysis of MR1 Expression

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10⁵ C1R cells overexpressing MR1 (C1R.MR1), described previously (43 (link)), were incubated with ligand for 3 or 16 h at 37 °C and 5% CO₂ in 100 μl or 200 μl RPMI 1640 medium from Gibco (Cat. No. 11875-093) supplemented with 10% fetal calf serum, 2% penicillin (100 U/ml), streptomycin (100 mg/ml), glutamax (2 mmol/L), sodium pyruvate (1 mmol/L), nonessential amino acids (0.1 mmol/L), Hepes buffer (15 mmol/L), pH 7.2 to 7.5 (all from ThermoFisher, Life Technologies), and 2-mercaptoethanol (50 mmol/L, Sigma) (RF-10). The cells were optionally stained with either Zombie Aqua Fixable Viability Kit from BioLegend (Cat. No. 423102) or Fixable Viability Dye eFluor 780 from ThermoFisher Scientific (Cat. No. 65-0865-14) and then incubated with biotinylated αMR1 antibody 26.5 (44 (link)). Unbound antibody was washed off with 2% fetal calf serum/PBS and the cells were incubated with PE- or APC-conjugated streptavidin (BioLegend, 30 μg/ml), washed again and fixed in 1% PFA/PBS. Data was acquired with an LSRFortessa X-20 (BD Biosciences) and Diva software (BD Biosciences) and analyzed using FlowJo software (BD Biosciences).

Wang C.J., Awad W., Liu L., Mak J.Y., Veerapen N., Illing P.T., Purcell A.W., Eckle S.B., McCluskey J., Besra G.S., Fairlie D.P., Rossjohn J, & Le Nours J. (2022). Quantitative affinity measurement of small molecule ligand binding to major histocompatibility complex class-I–related protein 1 MR1. The Journal of Biological Chemistry, 298(12), 102714.

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Peptide-HLA Class I Complexes Generation

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Peptide–HLA class I complexes were generated in-house by UV-mediated ligand exchange [48 (link)] followed by multimerization with PE- or APC-conjugated streptavidin (Biolegend, Koblenz, Germany).

Reguzova A., Fischer N., Müller M., Salomon F., Jaenisch T, & Amann R. (2021). A Novel Orf Virus D1701-VrV-Based Dengue Virus (DENV) Vaccine Candidate Expressing HLA-Specific T Cell Epitopes: A Proof-of-Concept Study. Biomedicines, 9(12), 1862.

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Multiparameter Flow Cytometry Analysis

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Single-cell suspensions were prepared from thymus, spleen or pooled lymph nodes. Cell surface markers, CD4, CD8, CD44, CD62L, GITR, NRP-1 and CD25 were detected using fluorescent conjugated antibodies from BioLegend, Tonbo and eBioscience diluted in (FACS) buffer. Intracellular staining for Foxp3 was done after fixing and permeabilizing cells (Tonbo Foxp3 staining kit), using antibodies from eBioscience and Tonbo. Complement deposition was detected using biotinylated antibodies for complement C1q, complement C3 and complement C4 from Cedarlane and PE or APC conjugated streptavidin from Biolegend and eBioscience. For complement experiments, freshly harvested splenocytes were incubated in GVB⁺⁺ buffer (Complement Technology) for one hour at room temperature and cells were washed with FACs buffer and stained with appropriate antibodies. All experiments included fixable viability dye (Tonbo) for analysis of live cells. Stained cells were analyzed using an Attune NxT flow cytometer (Thermofisher). Data was analyzed using FlowJo (Tree Star) 9.8 or 10. In all experiments, doublets and dead cells were excluded using permeable to fixable viability dye.

Dash B., Shapiro M.J., Chung J.Y., Arocha S.R, & Shapiro V.S. (2018). Treg-specific deletion of NKAP results in severe, systemic autoimmunity due to peripheral loss of Tregs. Journal of autoimmunity, 89, 139-148.

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