Lysozyme (Ly, Mw = 14.3 kDa) from chicken egg whites was purchased from MP Biomedicals, (Xi'an, China). Sodium carboxymethyl cellulose (CMC, Mw = 250 kDa) with the degrees of substitution (DS) of 0.7, 0.9 and 1.2 was purchased from Acros Organics (New Jersey, USA). AO/EB, 4′,6-diamidino-2-phenylindole (DAPI), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), and phosphate-buffered saline (PBS) were purchased from Heart Biological Technology CO., Ltd. (Xi'an, China). RPMI-1640 basal medium and fetal bovine serum (FBS) were purchased from HyClone (USA). The annexin V-FITC/PI apoptosis detection kit was obtained from Shanghai BestBio Institute (Shanghai, China). Other chemicals were of analytical grade and used without further purification. Aqueous solutions were prepared with ultrapure water (18.2 MΩ specific resistance), which was obtained from the Pall Cascada laboratory water system (Millipore, Bedford, MA, USA).
Sodium carboxymethyl cellulose
Manufactured by Thermo Fisher Scientific
Sourced in Belgium
Sodium carboxymethyl cellulose is a water-soluble polymer derived from cellulose. It is commonly used as a thickening, stabilizing, and emulsifying agent in various industrial and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
N hydroxysuccinimide, by Thermo Fisher Scientific (2 mentions)
1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride, by Thermo Fisher Scientific (2 mentions)
Lysozyme, by MP Biomedicals (1 mentions)
Iron nitrate, by Merck Group (1 mentions)
Manganese nitrate, by Merck Group (1 mentions)
Citric acid, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Methylene blue, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Zinc sulfate heptahydrate, by Thermo Fisher Scientific (1 mentions)
Ethylenediaminetetraacetic acid edta, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Pyrene, by Merck Group (1 mentions)
4 protocols using sodium carboxymethyl cellulose
1
Lysozyme and Carboxymethyl Cellulose Interaction
2
Manganese Spinel Ferrite Synthesis
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Manganese nitrate (Mn(NO3)2 ∙ 6H2O), iron nitrate (Fe(NO3)3 ∙ 9H2O), and citric acid (C6H8O7 ∙ H2O) were purchased from Merck Chemical (Saint Louis, MO, USA) and employed in manganese spinel ferrite synthesis. Sodium carboxymethyl cellulose (sodium salt, average M.W. 90000, DS = 0.7) was acquired from Acros Organics (Geel, Belgium), while sodium dodecyl sulfate (SDS), sodium chloride (NaCl), and Methylene Blue (MB) were supplied by Merck Chemical (Saint Louis, MO, USA). Ethanol was provided by Chemical Company (Iasi, Romania). These analytically graded materials were used without further purification.
3
Carboxymethyl Cellulose Conjugation Protocol
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Sodium carboxymethyl cellulose (Mw = 250
kDa; DS= 0.9), n-octylamine, and n-hydroxysuccinimide (NHS) were purchased from Acros Organics (Geel,
Belgium). Acetic acid, pyrene, and ethylenediaminetetraAcetic acid
(EDTA) were purchased from Sigma-Aldrich (Steinheim, Germany). 1-(3-(Dimethylamino)propyl)-3-ethylcarbodiimide
hydrochloride (EDC) was purchased from Alfa Aesar (Lancashire, United
Kingdom). Dextran standards were purchased from PSS Polymer (Mainz,
Germany). Zinc sulfate heptahydrate was purchased from Fisher chemicals
(Dublin, Ireland). Chitosan (Mw = 890 kDa; DD ≥ 90%) was purchased
from Glentham (Corsham, United Kingdom). Ethanol and ethanol absolute
were purchased from Gadot-group (Netanya, Israel). Water (HPLC grade)
was purchased from Bio Lab (Jerusalem, Israel). Deionized water (DW)
was obtained by mechanically filtering them through a Treion TS1173
column. Deuterated solvent for NMR analysis (D2O) was purchased
from Armar Chemicals (Döttingen, Switzerland). All regents
and solvents were used without further purification.
kDa; DS= 0.9), n-octylamine, and n-hydroxysuccinimide (NHS) were purchased from Acros Organics (Geel,
Belgium). Acetic acid, pyrene, and ethylenediaminetetraAcetic acid
(EDTA) were purchased from Sigma-Aldrich (Steinheim, Germany). 1-(3-(Dimethylamino)propyl)-3-ethylcarbodiimide
hydrochloride (EDC) was purchased from Alfa Aesar (Lancashire, United
Kingdom). Dextran standards were purchased from PSS Polymer (Mainz,
Germany). Zinc sulfate heptahydrate was purchased from Fisher chemicals
(Dublin, Ireland). Chitosan (Mw = 890 kDa; DD ≥ 90%) was purchased
from Glentham (Corsham, United Kingdom). Ethanol and ethanol absolute
were purchased from Gadot-group (Netanya, Israel). Water (HPLC grade)
was purchased from Bio Lab (Jerusalem, Israel). Deionized water (DW)
was obtained by mechanically filtering them through a Treion TS1173
column. Deuterated solvent for NMR analysis (D2O) was purchased
from Armar Chemicals (Döttingen, Switzerland). All regents
and solvents were used without further purification.
4
Bioconjugation of Insulin Using CMC
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Sodium carboxymethyl cellulose (MW = 250 kDa; DS = 0.9), n-dodecylamine and N-hydroxysuccinimide (NHS) were purchased from Acros Organics (Geel, Belgium). n-Hexylamine pyrene, insulin (monomer, human recombinant) and insulin labeled with fluorescein isothiocyanate (FITC; human recombinant) were purchased from Sigma Aldrich (Steinheim, Germany). 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was purchased from Alfa Aesar (Lancashire, UK). Dextran standards were purchased from PSS Polymer (Mainz, Germany). Ethanol and absolute ethanol were purchased from Gadot Group (Netanya, Israel). Water, high performance liquid chromatography (HPLC) grade, was purchased from Bio Lab (Jerusalem, Israel). Organic cold pressed sunflower oil (SO) was purchased from Joe & Co. (Vicenza, Italy). Deionized (DI) water was obtained by mechanically filtering water through a Treion TS1173 column. Deuterated solvent for the NMR analysis (D2O) was purchased from Armar Chemicals (Döttingen, Switzerland). All reagents and solvents were used without any further purification.
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