Briefly, cells (1×105 cells/ml) were treated with juglone (0, 12.5, 25, 50, or 100 μM) for 24 hr and then washed with PBS. After centrifugation, cells were fixed with 70% ethanol. Later, fixed cells were washed with PBS twice, and 50 μl RNAse and 25 μl propidium iodide (PI) were added to the cell suspension and incubated for 15 min. DNA content analysis was performed using Muse flow cytometry (Millipore, Billerica, MA, USA). SKOV3 cell apoptosis was analyzed using an AnnexinV-FITC/PI apoptosis detection kit (KeyGen Biotech Co. Ltd. Najing, China). The samples were stained with 5 μl Annexin V-FITC and 5 μl propidium iodide (PI) in the dark for 15 min at room temperature. Finally, samples were analyzed by Muse flow cytometry (Millipore, Billerica, MA, USA).
Muse flow cytometry
Manufactured by Merck Group
Sourced in United States
The Muse flow cytometry is a compact, automated cell analysis system that provides accurate and reproducible data on cell count, viability, and specific cellular characteristics. It utilizes flow cytometry principles to efficiently analyze cell samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using muse flow cytometry
1
Juglone-Induced Apoptosis in SKOV3 Cells
2
Cell Cycle Analysis of Garcinone-E Effects
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Flow cytometric studies were carried out to analyze various cell cycle check points in HeLa cells after garcinone-E exposure. HeLa cells were plated onto p60 tissue cultural dishes with a concentration of 1 × 104 cells/mL and cultured with variant garcinone-E doses (0, 16, 64 and 128 μM) for 24 h. After garcinone-E treatment, cells were harvested and washed with PBS followed by centrifugation of 5 min. Ethanol (70%) was then used to fix the centrifuged HeLa cells followed by double washing with PBS. After washing, suspensions were treated with RNAse and propidium iodide (Sigma-Aldrich) concentration of 50 μL and 25 μL respectively. Finally, Muse flow cytometry (Millipore, MA, United States) was used for DNA content quantification.
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