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Ethyl ether anhydrous

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Ethyl ether anhydrous is a clear, colorless, and volatile liquid laboratory reagent. It is commonly used as a solvent, extractant, and reaction medium in various chemical and analytical processes. The product is anhydrous, meaning it contains no water.

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Lab products found in correlation

Acryloyl chloride, by Merck Group (4 mentions) Anhydrous magnesium sulfate, by Thermo Fisher Scientific (3 mentions) Sodium chloride (nacl), by Thermo Fisher Scientific (3 mentions) Acetonitrile, by Thermo Fisher Scientific (3 mentions) Triethylamine, by Merck Group (3 mentions) Hydroquinone, by Merck Group (2 mentions) Ethyl acetate, by Thermo Fisher Scientific (2 mentions) Hexane, by Thermo Fisher Scientific (2 mentions) Sodium bicarbonate, by Thermo Fisher Scientific (2 mentions) Methanol, by Thermo Fisher Scientific (2 mentions) Chloroform, by Thermo Fisher Scientific (2 mentions) Dichloromethane, by Thermo Fisher Scientific (2 mentions) Lactide, by Merck Group (2 mentions) High purity chitosan, by Merck Group (2 mentions) Polyethylene glycol, by Merck Group (2 mentions) 1 ethyl 3 3 dimethylaminopropyl carbodiimide edc, by Thermo Fisher Scientific (2 mentions) Phosphate buffered saline ph 7, by Thermo Fisher Scientific (2 mentions) Ultrapure distilled water, by Thermo Fisher Scientific (2 mentions) 2 hydroxy 1 4 2 hydroxyethoxy phenyl 2 methyl 1 propanone irgacure d 2959, by Merck Group (2 mentions) Sulfated n hydroxysuccinimide sulfo nhs, by Thermo Fisher Scientific (2 mentions)

15 protocols using ethyl ether anhydrous

1

Quantifying Epidermal and Neuronal Tissue Damage

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To evaluate epidermal and neuronal tissue damage induced by HCl, third instar epidermal (e22c-Gal4, UAS-DsRed2Nuc,FasIII-GFP) and neuronal (ppk-Gal4, UAS-mCD8-GFP) reporter larvae were submerged in 100 µl of 9% HCl, anaesthetized with ether (ethyl ether anhydrous, Fisher Scientific), dissected in ice-cold phosphate-buffered saline (PBS), and then fixed for 1 h in 4% formaldehyde (FA). After several washes in PBS-Tx (1× phosphate-buffered saline with 0.3% Triton X-100) the samples were incubated overnight at 4°C with primary antibodies: mouse anti-GFP (1 : 500) for neurons; mouse anti-FasIII (1 : 50) and rabbit anti-DsRed (1 : 1000) for epidermal cells. Secondary antibodies (applied for 24 h at 4°C) were Alexa 647 anti-mouse (1 : 500) for neuronal samples and Alexa 488 anti-rabbit (1 : 500) and Alexa 647 anti-mouse (1 : 500) for epidermal tissues. After final washes in PBS-Tx, all stained samples were mounted in Vectashield (Vector Laboratories) and observed (see below).
Lopez-Bellido R., Himmel N.J., Gutstein H.B., Cox D.N, & Galko M.J. (2019). An assay for chemical nociception in Drosophila larvae. Philosophical Transactions of the Royal Society B: Biological Sciences, 374(1785), 20190282.
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2

Synthesis of Halogenated Monomers

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1,2-Dibromeoethane (99%), 1,3-dibromopropane (99%), 1,4-dibromobutane (99%), 1,5-dibromopentane (97%), 1,6-dibromohexane (98%), 1,7-dibromopentane (97%), 1,8-dibromooctane (98%), hydroquinone (99%), sodium hydrosulfite (technical grade), sodium sulfite (98%), 3-chloroperbenzoic acid (≤77%), thionyl chloride (99%), allyl bromide (99%), 4-hydroxybezoic acid (99%), and pyridine (anhydrous, 99.8%) were purchased from Sigma Aldrich and used without further purification. Sodium bicarbonate (99%), magnesium sulfate anhydrous (99%), sodium chloride (99%), hexanes (98.5%), ethyl acetate (99.5%) and ethyl ether anhydrous (99%), and methanol (ACS grade) were purchased from Fisher Chemical and used without further purification. Chloroform (HPLC grade), methylene chloride (HPLC), and acetonitrile (anhydrous), was purchased from Fisher Chemical and stored with activated molecular sieves to remove water. Sodium sulfate anhydrous (99%) and potassium hydroxide (85%) were purchased from Oakwood Chemical and used without further purification. Hydrochloric acid (10M) was purchased from Ricca Chemical and used without further purification. Sulfuric acid (95%) was purchases from VWR analytical and used without further purification. Ethanol anhydrous (99.9%) was purchased from Decon and used without further purification. Synthesis details of Cx diepoxy monomer is shown in SI Appendix.
Lv G., Shen C., Shan N., Jensen E., Li X., Evans C.M, & Cahill D.G. (2022). Odd–even effect on the thermal conductivity of liquid crystalline epoxy resins. Proceedings of the National Academy of Sciences of the United States of America, 119(46), e2211151119.
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3

Steroid Hormone and Angiotensin II Quantification

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Blood was collected at euthanasia and steroid hormones were extracted from 50μl of plasma using Ethyl Ether Anhydrous (Fisher Scientific) before being applied to commercial enzyme-linked immunosorbent assay (ELISA) kit. 17β-Estradiol and testosterone concentrations were determined by ELISA kits from Enzo Life Sciences (Cat# ADI-900–174 and ADI-900–065). Angiotensin II concentration was measured without extraction from plasma and was determined by ELISA kits from Enzo Life Sciences (Cat# ADI-900–204). The ELISA assays were conducted according to the manufacturer’s instructions.
Knowles R.B, & Kosik K.S. (1997). Neurotrophin-3 signals redistribute RNA in neurons. Proceedings of the National Academy of Sciences of the United States of America, 94(26), 14804-14808.
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4

Hydrogel Synthesis from NIPAAm and AMPS

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N-isopropylacrylamide (NIPAAm, 97%), 2-acrylamido-2-methylpropane sulfonic acid (AMPS, 97%), 1-vinyl-2-pyrrolidinone (NVP), N,N′-methylenebisacrylamide crosslinker (BIS, 99%), acryloyl chloride, triethylamine (Et3N), K2CO3, MgSO4, poly(ethylene glycol) (PEG; PEG-3400, MW = 3000–3700 g/mol per manufacturers specifications) and 2,2-di-methyl-2-phenyl-acetophenone (DMAP) were obtained from Sigma Aldrich. 1-[4-(2-Hydroxyethoxy)-phenyl]-2-hydroxy-2-methyl-1-propane-1-one (Irgacure® 2959) was purchased from BASF. Ethyl ether anhydrous was acquired from Fisher Scientific. For hydrogel fabrication, deionized water (DI) with a resistance of 18 MΩ·cm (Cascada LS MK2, Pall) was used. Phosphate-buffered saline (PBS, 1×, pH 7.4, without calcium and magnesium) was obtained from Corning®.
Means A.K., Dong P., Clubb FJ J.r., Friedemann M.C., Colvin L.E., Shrode C.A., Coté G.L, & Grunlan M.A. (2019). A self-cleaning, mechanically robust membrane for minimizing the foreign body reaction: towards extending the lifetime of sub-Q glucose biosensors. Journal of materials science. Materials in medicine, 30(7), 79.
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5

Peptide Synthesis and Purification Protocol

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Gold(III) chloride trihydrate, cetyltrimethylammonium bromide (CTAB), hydroquinone, sodium borohydride, silver nitrate, trifluoroacetic acid (TFA), 2,2′-(ethylenedioxy)diethanethiol, thioanisole (EODT), anisole, piperidine, trypsin from porcine pancreas (T4799), and bovine pancreatic trypsin inhibitor (BPTI) were purchased from Sigma-Aldrich. Fmoc-amino acids, Fmoc-Gly-Wang resin, and HBTU were received from Aapptec (Louisville, KY, USA). Diisopropylethylamine and ethyl ether anhydrous were purchased from Fisher Scientific. GCKGCG, GGKGGG, and GGKGCG were either purchased from Genscript Inc. (New Jersey, USA) or synthesized. Pooled human urine was collected from Innovative research (Novi, MI).
Creyer M.N., Jin Z., Moore C., Yim W., Zhou J, & Jokerst J.V. (2021). Modulation of Gold Nanorod Growth via the Proteolysis of Dithiol Peptides for Enzymatic Biomarker Detection. ACS applied materials & interfaces, 13(38), 45236-45243.
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6

Synthesis of Methylammonium Iodide

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Methylamine solution (CH3NH2, 40 wt.% in H2O), N-methyl-2-pyrrolidinone (NMP), and γ-Butyrolactone (GBL, ≥99%) were purchased from Aldrich. Hydriodic acid (HI, 57 wt.% in H2O) and lead(II) iodide (PbI2, 99.9985% metal basis) were purchased from Alfa Aesar. Ethyl ether (anhydrous) and acetone were obtained from Fisher Chemical. All chemicals were used as received without further purification. Equimolar of HI was dropwise added to CH3NH2 with stirring in a 50 mL round bottom flask immersed in ice bath, followed by rotary evaporation at 60 °C to dry off the solvent. Next, the white solid was obtained and washed with excessive ethyl ether on a filter paper, accompanied with vacuum filtration. The washed CH3NH3I powder was then dried in a vacuum oven at 60 °C overnight to yield the final product.
Guo P., Mannodi-Kanakkithodi A., Gong J., Xia Y., Stoumpos C.C., Cao D.H., Diroll B.T., Ketterson J.B., Wiederrecht G.P., Xu T., Chan M.K., Kanatzidis M.G, & Schaller R.D. (2019). Infrared-pump electronic-probe of methylammonium lead iodide reveals electronically decoupled organic and inorganic sublattices. Nature Communications, 10, 482.
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7

Fabrication of PLGA-PVA Nanoparticles

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PLGA 50:50 (Mw 5 7,000-17,000, ester terminated), poly(vinyl alcohol) (PVA, Mw 5 13,000-23,000), bovine serum albumin (BSA), sucrose (>99.5%), magnesium hydroxide (95%), PEG (Mn < 3,400), stannous octoate, acryloyl chloride (98%), 3,6-dimethyl-1,4-dioxane-2,5-dione, triethylamine (99.5%), and 2-hydroxy-2-methylpropiophenone (Irgacure 1173) were obtained from Sigma-Aldrich (St. Louis, MO). Dichloromethane (DCM, 99.9%), poly (ethylene glycol) (PEG, Mw 5 8,000), sodium chloride (99.5%), magnesium sulfate anhydrous (97%), ethyl ether (anhydrous), and phosphate-buffered saline (PBS) were obtained from Fisher Scientific (Hampton, NH).
Zivkovic L., Akar B., Roux B.M., Spremo Potparevic B., Bajic V, & Brey E.M. (2017). Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres. Journal of biomedical materials research. Part A, 105(1).
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8

Bioadhesive Hydrogel Tissue Bonding

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The starting materials included polyethylene glycol (Mw: 35 kDa), 2-hydroxy-1-[4-(2-hydroxyethoxy) phenyl]-2-methyl-1-propanone (Irgacure D-2959), lactide, triethylamine, acryloyl chloride, Tin(II) 2-ethylhexanoate [Sn(Oct)2], dichloromethane (anhydrous), toluene (anhydrous), potassium carbonate (K2CO3), calcium chloride (CaCl2) and magnesium sulfate (MgSO4) purchased from Sigma-Aldrich (MO, USA), ethyl ether (anhydrous) purchased from Thermo Fisher Scientific (MA, USA), micro-filtered and lyophilized sodium alginate (MVG GRGDSP-coupled; high G ratio; high Mw) purchased from NovaMatrix (Dupont, DE, USA), high purity chitosan (99% degree of deacetylation; Mw: 100 kDa), as primers for tissues, purchased from Sigma-Aldrich (MO, USA), and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and sulfated N-hydroxysuccinimide (Sulfo-NHS), as coupling reagents for tissue and device primers, purchased from Thermo Fisher Scientific (MA, USA). The solvents included ultrapure distilled water, phosphate-buffered saline (PBS; pH 7.4), and 2-(N-morpholino)ethanesulfonic acid (MES; pH 5.0) purchased from Thermo Fisher Scientific (MA, USA). All chemicals were used as received. Tissues and organs for bench studies were purchased from Animal Biotech Industries (ABI; PA, USA) and were cleaned with soap and water before adhesion measurements.
Yang Q., Wei T., Yin R.T., Wu M., Xu Y., Koo J., Choi Y.S., Xie Z., Chen S.W., Kandela I., Yao S., Deng Y., Avila R., Liu T.L., Bai W., Yang Y., Han M., Zhang Q., Haney C.R., Lee K.B., Aras K., Wang T., Seo M.H., Luan H., Lee S.M., Brikha A., Ghoreishi-Haack N., Tran L., Stepien I., Aird F., Waters E.A., Yu X., Banks A., Trachiotis G.D., Torkelson J.M., Huang Y., Kozorovitskiy Y., Efimov I.R, & Rogers J.A. (2021). Photocurable bioresorbable adhesives as functional interfaces between flexible bioelectronic devices and soft biological tissues. Nature materials, 20(11), 1559-1570.
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9

Bioadhesive Hydrogel Tissue Bonding

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The starting materials included polyethylene glycol (Mw: 35 kDa), 2-hydroxy-1-[4-(2-hydroxyethoxy) phenyl]-2-methyl-1-propanone (Irgacure D-2959), lactide, triethylamine, acryloyl chloride, Tin(II) 2-ethylhexanoate [Sn(Oct)2], dichloromethane (anhydrous), toluene (anhydrous), potassium carbonate (K2CO3), calcium chloride (CaCl2) and magnesium sulfate (MgSO4) purchased from Sigma-Aldrich (MO, USA), ethyl ether (anhydrous) purchased from Thermo Fisher Scientific (MA, USA), micro-filtered and lyophilized sodium alginate (MVG GRGDSP-coupled; high G ratio; high Mw) purchased from NovaMatrix (Dupont, DE, USA), high purity chitosan (99% degree of deacetylation; Mw: 100 kDa), as primers for tissues, purchased from Sigma-Aldrich (MO, USA), and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and sulfated N-hydroxysuccinimide (Sulfo-NHS), as coupling reagents for tissue and device primers, purchased from Thermo Fisher Scientific (MA, USA). The solvents included ultrapure distilled water, phosphate-buffered saline (PBS; pH 7.4), and 2-(N-morpholino)ethanesulfonic acid (MES; pH 5.0) purchased from Thermo Fisher Scientific (MA, USA). All chemicals were used as received. Tissues and organs for bench studies were purchased from Animal Biotech Industries (ABI; PA, USA) and were cleaned with soap and water before adhesion measurements.
Yang Q., Wei T., Yin R.T., Wu M., Xu Y., Koo J., Choi Y.S., Xie Z., Chen S.W., Kandela I., Yao S., Deng Y., Avila R., Liu T.L., Bai W., Yang Y., Han M., Zhang Q., Haney C.R., Lee K.B., Aras K., Wang T., Seo M.H., Luan H., Lee S.M., Brikha A., Ghoreishi-Haack N., Tran L., Stepien I., Aird F., Waters E.A., Yu X., Banks A., Trachiotis G.D., Torkelson J.M., Huang Y., Kozorovitskiy Y., Efimov I.R, & Rogers J.A. (2021). Photocurable bioresorbable adhesives as functional interfaces between flexible bioelectronic devices and soft biological tissues. Nature materials, 20(11), 1559-1570.
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10

Cationic Lipid Nanoparticle Synthesis

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Branched PEI (25,000 Da), triethylamine (TEA), oleoyl chloride (OA, 99%) and FA were obtained from Sigma–Aldrich (St. Louis, MO). Anhydrous ethyl ether and dichloromethane (DCM) were purchased from Fisher Scientific (Fairlawn, NJ). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Sigma–Aldrich (St. Louis, MO). Fetal bovine serum (FBS) was obtained from Gibco BRL (Bethesda, MD). 4′,6-Diamidino-2-phenylindole (DAPI) was purchased from Invitrogen Molecular Probes (Eugene, OR). LOR-2501 (5′-CTC TAG CGT CTT AAA GCC GA-3′) and 5′-Cy3-LOR-2501 were obtained from Biomics Biotechnologies (Nantong, Jiangsu).
Yang S., Yang X., Liu Y., Zheng B., Meng L., Lee R.J., Xie J, & Teng L. (2015). Non-covalent complexes of folic acid and oleic acid conjugated polyethylenimine: An efficient vehicle for antisense oligonucleotide delivery. Colloids and surfaces. B, Biointerfaces, 135, 274-282.
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