Rotipuran supra

Triapine,^{40 (link)} Me₂NNMe₂,^{36 (link)} and Dp44mT^{41 (link)} were prepared as described previously. Ultrapure water (18.2 MΩ cm, ELGA Water purification system, Purelab Ultra MK 2, UK or 18.2 MΩ cm, Milli-Q Advantage, Darmstadt, Germany), nitric acid (≥69%, Rotipuran Supra, Carl Roth, Karlsruhe, Germany), and H₂O₂ (30%, Suprapur, Merck, Darmstadt, Germany) were used for the dilutions in the direct infusion ICP-MS measurement. The multielement standard solution, containing 26 elements (Quality Control Standard 26), was purchased from Labkings (Hilversum, The Netherlands). For validation purposes, the certified reference materials TM 28.4, Lake Ontario water, (Environment and Climate change, Burlington, Canada) and Seronorm^TM, trace elements serum L-1, (Sero AS, Billingstad, Norway) were used. The ammonium acetate buffer applied as eluent for chromatography was derived from ammonium hydroxide solution (25%, Suprapur) and acetic acid (100%, Suprapur), which were purchased by Lactan (Graz, Austria). The protein standards used in the characterization of human serum were all purchased by Athens Research and Technology (Georgia, USA). N,N-Dimethylformamide (≥99.9%), Na₂HPO₄ (≥99%, EMSURE^®), NaH₂PO₄ (≥99%, EMSURE^®), human serum (from human male AB plasma, USA origin), and fetal calf serum (USA origin) were all purchased by Sigma Aldrich (Steinheim, Germany).

Ultrapure water (18.2 MΩ cm, ELGA Water Purification System, Purelab Ultra MK 2, UK or 18.2 MΩ cm, Milli-Q Advantage, Darmstadt, Germany) and nitric acid (>69%, Rotipuran Supra, Carl Roth, Karlsruhe, Germany) were used for all dilutions for ICP-MS measurements. For digestion, H₂O₂ (30%, Suprapur, Merck, Darmstadt, Germany) was added. A multi-element stock solution and single-element standard solutions were purchased from LabKings (Hilversum, The Netherlands). Gelatin (from cold water fish skin) was obtained from Sigma-Aldrich (Vienna, Austria). Pooled human plasma was purchased from Dunn Labortechnik (Asbach, Germany). The serum reference material Seronorm (Seronorm Trace Elements Serum L-1, Norway) was reconstituted according to the manufacturer’s protocol. TM-28.4 Lake Ontario water certified reference material (Environment and Climate Change, Burlington, Canada) was used. Sample preparation and measurements were carried out in clean room classes 100.000 and 10.000, respectively. All cell culture media and reagents were purchased from Sigma-Aldrich (Vienna, Austria) and all plastic dishes, plates, and flasks from StarLab (Hamburg, Germany) unless stated otherwise. Cisplatin and oxaliplatin were synthesized at the Institute of Inorganic Chemistry, University of Vienna, according to literature procedures [28 (link), 29 ].

The accelerated
degradation tests (ADTs) were performed in a setup already described
as a part of our previous work (see also SI, Figure S1).^{31 (link)} Briefly, the setup is composed
of a two-compartment HT-cell using 0.1 M HClO₄ electrolyte
(Carl Roth, Rotipuran Supra) with a conventional three-electrode system
controlled by a potentiostat (CompactStat, Ivium Technologies). The
reversible hydrogen electrode (HydroFlex, Gaskatel) was used as a
reference (separated from the working electrode in a different compartment
via a salt-bridge), and a graphite rod was used as a counter electrode
(with respect to the temperature at which ADTs were performed, a fresh
graphite rod was used for each measurement).