Rtx 5ms

Manufactured by Shimadzu

Sourced in Japan

The Rtx-5MS is a high-performance gas chromatography (GC) column designed for a wide range of applications. It features a 5% diphenyl and 95% dimethylpolysiloxane stationary phase, which provides excellent separation and inertness for a variety of analytes. The Rtx-5MS column is suitable for the analysis of various organic compounds, including environmental pollutants, pharmaceuticals, and food additives.

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Lab products found in correlation

Gcms qp2010, by Shimadzu (5 mentions) Gcms qp2010 plus, by Shimadzu (3 mentions) Qp2010, by Shimadzu (3 mentions) Gcms qp2010 ultra, by Shimadzu (2 mentions) Qp 2010 plus system, by Shimadzu (2 mentions) Qp 2010 system, by Shimadzu (2 mentions) Qp2010 ultra, by Shimadzu (1 mentions) Aoc 20, by Shimadzu (1 mentions) Aoc 20i, by Shimadzu (1 mentions) Gc ms 2010, by Shimadzu (1 mentions) 2010 plus, by Shimadzu (1 mentions) Gas chromatograph mass spectrometer, by Shimadzu (1 mentions)

Close spelling variants of this product

Rtx-5MS capillary column (32 citations) Rtx-5MS column (32 citations) Rtx-5MS fused bonded column (11 citations) 60 M RTX 5MS column (3 citations) SH-Rtx-5MS column (3 citations) SH-Rtx-5MS (3 citations) Rtx-5MS silica capillary column (3 citations) Ultra Rtx-5MS (2 citations)

21 protocols using rtx 5ms

Volatile Organic Metabolites Analysis in Sourdough

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VOMs were analyzed using a head space SPME-GC-MS: 0.5 g of sourdough was mixed with 0.5 mL of water, 0.5 g of NaCl, and 10 μL of 2-octanol (100 μg/mL, internal standard) in a 10 mL sample bottle (Supelco, Bellefonte, PA, USA). The mixture was magnetic stirred (750 r/min) at 50 °C for 10 min and then subjected to solid-phase microextraction (SPME) using the DVB/Carboxen/PDMS (50/30 μm; Supelco, Bellefonte, PA, USA) fiber with the exposure time of 30 min.
GC-MS was conducted using GCMS-QP2010 Plus (SHIMADZU, Kyoto, Japan) equipped with a capillary column (Rtx-5ms, 30 m × 0.25 mm × 0.25 μm; SHIMADZU, Kyoto, Japan). The operating parameters were set as follows: splitless mode; the injection, transfer line, and ion source temperatures were 230, 280, and 230 °C, respectively; carrier gas (helium) flow at 1.0 mL/min; and EI, 70 eV. The oven was programmed as follows: 35 °C held for 2 min, increased to 180 °C at 5 °C/min, and then increased to 250 °C at 20 °C/min and held for 5 min. The MS scan range was 30–1000 amu [20 (link)].

Guo W., Li Z., Fu X., Zhou W., Ren J, & Wu Y. (2022). Effect of Staphylococcus aureus Contamination on the Microbial Diversity and Metabolites in Wholewheat Sourdough. Foods, 11(13), 1960.

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Chemical Composition of Essential Oils

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Ten essential oils used in this study were commercially obtained from Brazilian industry (Ferquímica^®, Vargem Grande Paulista, SP, Brazil): star anise (Illicium verum), citronella (Cymbopogon winterianus), clove bud (Eugenia caryophyllus), staigeriana eucalyptus (Eucalyptus staigeriana), globulus eucalyptus (Eucalyptus globulus), ginger (Zingiber officinale), ho wood (Cinnamomum camphora), melaleuca (Melaleuca alternifolia), oregano (Origanum vulgare), and white thyme (Thymus vulgaris). The chemical composition of EO was carried out in order to identify the main bioactive compounds according to Wiley Library (Version 8), using a gas chromatograph coupled to a mass spectrometer (GC/MS QP2010 Plus, Shimadzu, Kyoto, Japan), with a diphenyl-dimethyl-polysiloxane capillary column (5% diphenyl and 95% dimethyl polysilozane) with 30 m × 0.25 mm, 0.25 μm, model Rtx^®-5MS (Bellefonte, PA, USA) at the Multi-User Laboratory for Biochemistry and Instrumental Analysis of the Department of Agribusiness, Food and Nutrition at ESALQ/USP (Piracicaba, Brazil).

Benetel G., Silva T.D., Fagundes G.M., Welter K.C., Melo F.A., Lobo A.A., Muir J.P, & Bueno I.C. (2022). Essential Oils as In Vitro Ruminal Fermentation Manipulators to Mitigate Methane Emission by Beef Cattle Grazing Tropical Grasses. Molecules, 27(7), 2227.

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GC-MS Analysis of Organic Compounds

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Gas chromatography coupled with mass spectrometry (GC/MS) analyses were performed on a Shimadzu GCMS-QP 2010 (Shimadzu Corporation, Koyoto, Japan), provided using an Rtx-5MS (30 m × 0.25 mm i.d. × 0.25 µm film thickness) capillary column (Restek, Bellefonte, PA, USA) and attached to a Shimadzu mass spectrometer. The column temperature was initially set at 50 °C for 3 min. Then, the temperature was gradually increased from 50 to 300 °C at a rate of 5 °C/min and then isothermally maintained at 300 °C for 10 min. The temperature of the injector was kept at 280 °C. Helium was used as a carrier gas at a flow rate of 1.37 mL/min. The ion source and the interface were at temperatures of 280 and 220 °C, respectively. An injection of 1 µL of 1% v/v of diluted sample was achieved via a split mode adopting a split ratio of 15:1. Recording of the mass spectrum was performed in EI mode of 70 eV from m/z 35 to 500. Compound quantitation was performed based on the normalisation method, employing the reading of three chromatographic runs.

Aly S.H., El-Hassab M.A., Elhady S.S, & Gad H.A. (2022). Comparative Metabolic Study of Tamarindus indica L.’s Various Organs Based on GC/MS Analysis, In Silico and In Vitro Anti-Inflammatory and Wound Healing Activities. Plants, 12(1), 87.

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GC-FID Analysis of Headspace Volatiles

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The HS was analyzed on a Shimadzu GC system QP2010 Ultra equipped with a FID detector using a fused capillary column Rtx-5MS, length 30 m, internal diameter 0.25 mm, and film thickness 0.25 μm (Shimadzu, Kyoto, Japan). Ultra-high purity helium (99.99%) (National Industrial Gas Plants, Salwa, Sudan) was used as the carrier gas at a constant flow rate of 1 mL/min. The column oven temperature was programmed at 35°C having a holding time of 3 min whereas the injection temperature was set at 250°C. The injection mode was split with a ratio of 100 and the flow control mode had a linear velocity of 39.4 cm. The total flow rate was 124 mL/min, whereas the column flow rate and purge flow rate were 1.2 and 3.0 mL/min, respectively, at 61.8 kPa pressure. The other parameters include ion source temperature of 200°C, interface temperature of 250°C and solvent cut time of 3.5 min. The MS start time was 4 min and the end time was 61.3 min. The acquisition mode was operated at 1,666 scan speed with 0.5 s event time. Sample was run in the 35~800 m/z range and the total ion chromatogram obtained was auto-integrated using ChemStation. The components were identified by comparison with the National Institute of Standards and Technology (NIST) and Wiley 7 libraries. The relative percentage amount of each component was calculated by comparing its average peak area to the total areas.

Hossain S.J., Islam M.R., Pervin T., Iftekharuzzaman M., Hamdi O.A., Mubassara S., Saifuzzaman M, & Shilpi J.A. (2017). Antibacterial, Anti-Diarrhoeal, Analgesic, Cytotoxic Activities, and GC-MS Profiling of Sonneratia apetala (Buch.-Ham.) Seed. Preventive Nutrition and Food Science, 22(3), 157-165.

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GC-MS Analysis of Essential Oils

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Essential oil analysis was performed by gas chromatography coupled with mass spectrometry (GC/MS) in a GCMS-QP2010 Ultra, Shimadzu, equipped with an autosampler, AOC-20s, Shimadzu, auto-injector, AOC-20i, Shimadzu, and RTX-5MS (30 m × 0.25 mm ID and 0.25 μm film thickness) columns. The injector temperature was set at 200 °C. The oven temperature was initially at 70 °C for 2 min, programmed to reach 180 °C at the rate of 20 °C/min and held at 180 °C for 3 min, then increased to 250 °C at the rate of 20 °C/min, and finally kept constant at 250 °C for 16 min. Helium was used as the carrier gas with a 35.2 mL/min flow and 100 kPa pressure. The sample (0.2 μL) was neatly injected with a 20:1 split ratio, and the mass spectrometer was operated in the electron impact (EI) mode at 70eV. Thus, the mass scanning range was varied over 35-500 m/z. Also, the ion source and quadrupole temperatures were set at 230 and 150 °C, respectively. The essential oil components were identified based on their mass spectral fragmentation using the Wiley 9 GC/MS libraries. Subsequently, the identified compound's percentage was computed from a total ion chromatogram [16] .

Muderawan I.W., Mudianta I.W., & Martiningsih N.W. (2022). Physicochemical Properties, Chemical Compositions and Antioxidant Activities of Rhizome Oils from Two Varieties of <i>Kaempferia galanga</i>. Indonesian Journal of Chemistry, 22(1), 72-72.

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Quantifying Heptachlor and Metabolites by GC-MS

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The concentrations of heptachlor and its metabolites were analyzed by a Shimadzu GC/MS2010 apparatus with a RTX-5MS from Shimadzu (30 m × 0.25 mm × 0.25 mm). The column temperature program was 80 °C isothermal for 5 min and then from 80 °C to 260 °C with an increment of 10 °C min⁻¹. The MS energy was derived from a 70 eV electron ionization source of electron bombardment; ion source temperature was maintained at 200 °C; interface temperature was maintained at 260 °C; mass scan range was set at 40–450 m/z.
Samples were prepared in a 40 mL brown reagent bottle. A volume of 10 mL sample contained heptachlor and its metabolites obtained after the pretreatment of the bacterial cultures was placed into the reagent bottle, and then five milliliter n-hexane was added.^12,13 The bottle was kept in a constant-temperature rotating shaker (HZQ-C, China) at 200 rpm for 20 min and then in ultrasonic waves at 28 kHz frequency for 10 min for heptachlor extraction. The extraction process was repeated three times. The organic fraction was dried over anhydrous sodium sulfate. Finally, 1 μL of the dry organic sample that passed through an organic filter (hydrophobic PTFE) of 0.22 μm pore size was analyzed by GC/MS.
The cell concentrations of all samples were detected using Visible Spectrophotometry and Petroff-Hausser counting chamber.¹⁴

Qiu L., Wang H, & Wang X. (2018). Conversion mechanism of heptachlor by a novel bacterial strain. RSC Advances, 8(11), 5828-5839.

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Essential Oil Analysis by GC-MS

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An analysis of each essential oil was carried out separately via gas chromatography-mass spectrometry (GC-MS) where the procedure was adopted from a previous work [29 (link)]. A system operating Shimadzu GCMS-QP2010 (Tokyo, Japan) was used with the following conditions: The column (RTX-5 MS) was used with specifications that were (30 m × 0.25 mm i.d. × 0.25 µm film thickness) (Restek Corporation, Bellefonte, Pennsylvania, USA). The starting temperature of the column was 45 °C for 2 min and then increased to 300 °C at a rate of 5 °C/min and kept steady for 5 min. The temperature of the injector was 250 °C. The flow rate of helium (carrier gas) was (1.41 mL/min). The following conditions were applied when recording the mass spectra: (equipment current) filament emission current, 60 mA; ionization voltage, 70 eV; ion source, 200 °C. Automatic injection of the essential oil was at (1 µL, 1% v/v) with a splitting ratio (1:15). The identification of volatile metabolites was performed upon comparing the mass spectra as well as the retention index with those of the National Institute of Standards and Technology’s (NIST) chemistry webbook library. In addition, literature data was used to identify n-alkanes series by comparing their mass spectra and retention indices.

El-Kased R.F, & El-Kersh D.M. (2022). GC–MS Profiling of Naturally Extracted Essential Oils: Antimicrobial and Beverage Preservative Actions. Life, 12(10), 1587.

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Chemical Composition Analysis of Essential Oils

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The chemical composition of essential oils was evaluated by gas chromatography-mass spectrometry (GC/MS), using a Shimadzu QP-2010 plus system with the subsequent conditions: silica capillary column Rtx-5MS (30 m × 0.25 mm, film thickness 0.25 μm); programmed temperature (60–240) °C to 3 °C/min; injector temperature of 250 °C; helium as carrying gas (linear velocity of 32 cm/s, measured at 100 °C); and injection without division (1 μL of a hexane 2:1000 solution). The ionization was performed by the electron impact technique (70 eV), and the ion source temperature, along with the other parts, was fixed to 200 °C. The quantification of volatile compounds was determined by gas chromatography with a flame ionization detector (FID; Shimadzu, Kyoto, Japan, system QP 2010) at the very same conditions as expressed for GC/MS, except for the carrying gas, which was hydrogen. The retention index was calculated for every volatile constituent eluting a homologous series of n-alkanes (C₈–C₄₀), and the spectra obtained experimentally were compared with literature, alongside the retention indexes [46 ,72 ,73 ].

da Silva L.R., Correia Z.A., Gurgel E.S., Ribeiro O., Silva S.G., Ferreira O.O., Andrade E.H, & de Oliveira M.S. (2023). Morphoanatomical, Histochemical, and Essential Oil Composition of the Plectranthus ornatus Codd. (Lamiaceae). Molecules, 28(18), 6482.

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GC-MS Analysis of Volatile Compounds in Rose Mutants

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The volatile compound compositions were analyzed using a GC-MS (Plus-2010, Shimadzu, Kyoto, Japan) equipped with a Rtx-5MS (30 m × 0.32 mm × 50 µm, Shimadzu, Kyoto, Japan) column. The carrier gas was 99.99% high-purity helium with a column flow rate of 1.37 mL/min. Sample injection was performed in splitless mode. The oven temperature was initially set at 40 °C, and was gradually increased to 300 °C at 5 °C/min with a final hold for 5 min. The mass spectrometry parameters included: Electron-impact ionization, 70 eV; ion source temperature, 230 °C; scan range, 40–500. The identification of each compound was performed using mass spectral libraries and Kovats retention indexes (RI). The GC-MS analysis detected volatile compounds in the rose mutants and those of the original cultivars, and compounds were tentatively identified based on a NIST library similarity index greater than 90%. The retention indices of all GC peaks were calculated with retention times of C7–C40 saturated alkane standards under the same chromatographic conditions. The RI of each compound on each column was calculated using the formula; y and z are carbon numbers of alkane standards, T_(x) is the retention time of the compound, and n and n + 1 represent the retention times of the alkane standards.

R I = 100 y + 100 (z - y) \times (\frac{T_{(x)} - T_{(n)}}{T_{n + 1} - T_{n}})

Ryu J., Lyu J.I., Kim D.G., Kim J.M., Jo Y.D., Kang S.Y., Kim J.B., Ahn J.W, & Kim S.H. (2020). Comparative Analysis of Volatile Compounds of Gamma-Irradiated Mutants of Rose (Rosa hybrida). Plants, 9(9), 1221.

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GLC-MS Analysis of Organic Compounds

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GLC-MS analysis was achieved on Shimadzu GCMS-QP 2010 (Shimadzu Corporation, Koyoto, Japan) equipped with Rtx-5MS (30 m × 0.25 mm i.d. × 0.25 µm film thickness) capillary column (Restek, PA, USA) and coupled to a Shimadzu mass spectrometer. Initial column temperature was set at 50 °C for 3 min; 50–300 °C at a rate of 5 °C/min and then 10 min isothermal at 300 °C. The injector temperature was 280 °C. The carrier gas (helium) flow rate was set at 1.37 mL/min. The interface and ion source temperatures were adjusted to 280 and 220 °C, respectively. Diluted samples (1% v/v) were injected in split mode with split ratio 15:1. The injection volume was 1 μL. Mass spectra were recorded in EI mode of 70 eV, scanning from m/z 35 to 500.

Ayoub I.M., Korinek M., El-Shazly M., Wetterauer B., El-Beshbishy H.A., Hwang T.L., Chen B.H., Chang F.R., Wink M., Singab A.N, & Youssef F.S. (2021). Anti-Allergic, Anti-Inflammatory, and Anti-Hyperglycemic Activity of Chasmanthe aethiopica Leaf Extract and Its Profiling Using LC/MS and GLC/MS. Plants, 10(6), 1118.

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