Hrp conjugated goat anti rabbit igg antibody

Manufactured by Santa Cruz Biotechnology

Sourced in United States, Germany

The HRP-conjugated goat anti-rabbit IgG antibody is a laboratory reagent used for the detection of rabbit immunoglobulin G (IgG) in various immunoassays. The antibody is conjugated with horseradish peroxidase (HRP), an enzyme that can catalyze a colorimetric reaction, allowing for the visualization and quantification of target proteins.

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Lab products found in correlation

Hrp conjugated goat anti mouse igg antibody, by Santa Cruz Biotechnology (4 mentions) Ecl plus kit, by Cytiva (2 mentions) Pvdf membrane, by Bio-Rad (2 mentions) Quantity one, by Bio-Rad (2 mentions) Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) Dmso d6, by Merck Group (1 mentions) Phospho p38, by Cell Signaling Technology (1 mentions) Phospho jnk antibody, by Cell Signaling Technology (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Cpla2, by Cell Signaling Technology (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions) Pvp k30, by Merck Group (1 mentions) Mmp 9, by Santa Cruz Biotechnology (1 mentions) Cox 2, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Immobilon p polyvinylidene difluoride membrane, by Merck Group (1 mentions) Clarity western ecl substrate, by Bio-Rad (1 mentions) Rabbit anti akt, by Cell Signaling Technology (1 mentions) Rabbit anti gapdh, by Proteintech (1 mentions)

Close spelling variants of this product

Goat anti-rabbit HRP (55 citations) HRP-conjugated goat anti-rabbit antibody (30 citations) HRP-conjugated goat anti-rabbit (27 citations) HRP-conjugated anti-rabbit antibody (23 citations) Goat anti-rabbit IgG-HRP antibody (17 citations) HRP-conjugated goat anti-rabbit IgG secondary antibody (15 citations) Horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (10 citations) Goat anti-rabbit or mouse IgG-HRP conjugated secondary antibody (7 citations) Rabbit anti-IgG (4 citations) Horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody (4 citations)

21 protocols using hrp conjugated goat anti rabbit igg antibody

Synthesis and Characterization of (734THI) Compound

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734THI was synthesized by Assistant Professor Chih-Hua Tseng, College of Pharmacy, Kaohsiung Medical University. PVP K30 (average molecular weight 40,000, K-value 29–32), dimethyl sulfoxide (DMSO) and DMSO-d6 were obtained from Sigma (St Louis, MO, USA). The HaCaT keratinocyte cell line was supplied by Professor Jeff Yi-Fu Chen, Department of Biotechnology, Kaohsiung Medical University. DMEM/F12 was obtained from Thermo Fisher Scientific (Waltham, MA, USA). PM (Standard Reference Material^® 1649b) was purchased from the National Institute of Standards and Technology (Gaithersburg, MD, USA). Primary antibodies, COX-2, ICAM, MMP-9, GAPDH and HRP-conjugated goat anti-rabbit IgG antibodies, were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). cPLA2, phospho-ERK, phospho-p38 and phospho-JNK antibodies were purchased from Cell Signaling (Danvers, MA, USA). All other chemical reagents were of analytical grade.

Huang P.H., Tseng C.H., Lin C.Y., Lee C.W, & Yen F.L. (2018). Preparation, characterizations and anti-pollutant activity of 7,3′,4′-trihydroxyisoflavone nanoparticles in particulate matter-induced HaCaT keratinocytes. International Journal of Nanomedicine, 13, 3279-3293.

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Immunoblotting Analysis of H. pylori Antigens

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Whole cell lysates were prepared from bacterial cells standardised to OD₆₀₀ of 10. For each blot, 10 µl of sample was used for SDS-PAGE, followed by transfer to an Immobilon^®-P polyvinylidene difluoride membrane (Merck Millipore). Blood group antigen-binding adhesin A (BabA) detected with polyclonal antibody, with detection by horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibodies (Santa Cruz Biotechnology) and visualization by chemiluminescent signal using Clarity™ Western ECL substrate (Bio-Rad) on a Fujifilm LAS-3000 imaging system. H. pylori UreB was detected using rabbit anti-urease B polyclonal antibodies purchased from Sigma Aldrich. H. pylori Le antigen phenotypes were determined using monoclonal antibodies (Abcam) to Le^a, Le^b, Le^x, or Le^y. Bound immunoglobulin M (IgM) or IgG antibodies were detected with HRP-conjugated goat anti-mouse antibodies (Sigma Aldrich) against IgM or IgG.

Chua E.G., Wise M.J., Khosravi Y., Seow S.W., Amoyo A.A., Pettersson S., Peters F., Tay C.Y., Perkins T.T., Loke M.F., Marshall B.J, & Vadivelu J. (2016). Quantum changes in Helicobacter pylori gene expression accompany host-adaptation. DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes, 24(1), 37-49.

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Western Blot Analysis of Cell Signaling Proteins

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Cells were harvested and lysed in ice-cold lysis buffer (50 mM Tris, 2% SDS, 5% glycerinum, 100 mM NaCl, and 1 mM EDTA, pH 6.8). The total protein content of the lysate was measured using a BCA Protein Assay Kit (Pierce Biotechnology, Cat no. 23235). Equal amounts of protein (30 μg) were separated by SDS/10% PAGE; after which, the protein bands were transferred onto a PVDF membrane (BioRad, Cat no. 162-0177), which was then incubated with rabbit anti-FBXW7 (Proteintech, Cat no. 117515-1-AP; 1:1000 dilution), rabbit anti-IGF1R (Cell Signaling Technology, Cat no. 4668; 1:1000 dilution), rabbit anti-p-AKT (Cell Signaling Technology, Cat no. 9252; 1:1000 dilution), rabbit anti-AKT (Cell Signaling Technology, Cat no. 9215; 1:500 dilution), rabbit anti-Notch (Cell Signaling Technology, Cat no. 9212; 1:1000 dilution), and rabbit anti-GAPDH (Proteintech, Cat no. 10494-1-AP; 1:1000 dilution) antibodies overnight at 4°C. The membrane was then incubated with horseradish-peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (Santa Cruz Biotechnology, Cat no. SC-2054; 1:5000 dilution). The signal intensity of each protein band was measured with an ECL-PLUS/Kit (Amersham, Cat no. RPN2132) according to the manufacturer’s protocol. GAPDH served as an internal control standard. The relative density of bands were quantified by quantity one (Bio-Rad).

Zhang H., Chen F., He Y., Yi L., Ge C., Shi X., Tang C., Wang D., Wu Y, & Nian W. (2017). Sensitivity of non-small cell lung cancer to erlotinib is regulated by the Notch/miR-223/FBXW7 pathway. Bioscience Reports, 37(3), BSR20160478.

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Sandwich ELISA for rGAPDH in Rat Kidney

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A sandwich ELISA to detect the rGAPDH as a mid-term PMI marker in rat kidney was performed using different epitope polyclonal antibodies. Briefly, the ELISA plates were coated with 50 μL goat polyclonal GAPDH antibody (0.5 μg/mL, sc-20357, Santa Cruz Biotechnology) and incubated at 4°C overnight. Tris-base saline containing 1% (w/v) BSA was added to prevent non-specific binding for 2 h at room temperature. The plates were washed with TBST buffer. Serially diluted rGAPDH proteins are added to the plates at 0–100 ng/μL and incubated for 1 h 30 min at 37°C. After washing, rabbit polyclonal GAPDH antibody (1:1000, sc-25778, Santa Cruz Biotechnology) was added and incubated for 2 h at room temperature followed by washing. Bound antibodies were detected with HRP-conjugated goat anti-rabbit IgG antibody (1:1000, sc-2004, Santa Cruz Biotechnology) and revealed using 3,3’,5,5’-tetramethylbenzidine dye. Absorbance was measured at 450 nm using a microplate reader (Sunrise^TM, Tecan, Basel, Switzerland).

Lee D.G., Yang K.E., Hwang J.W., Kang H.S., Lee S.Y., Choi S., Shin J., Jang I.S., An H.J., Chung H., Jung H.I, & Choi J.S. (2016). Degradation of Kidney and Psoas Muscle Proteins as Indicators of Post-Mortem Interval in a Rat Model, with Use of Lateral Flow Technology. PLoS ONE, 11(8), e0160557.

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Western Blot Analysis of Retinal Proteins

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A week after MNU administration, the retinas were cut into pieces and homogenized in buffer containing 0.23 mmol sucrose, 2 mmol EDTA, 5 mmol Tris–HCl (pH 7.5), and 0.1 mmol phenylmethylsulfonyl fluoride. After centrifugation, aliquot extracts containing equal amounts of protein (20 µg) were electrophoresed, transferred, and probed with a rabbit anti-rat polyclonal anti-mouse antibody (1:500, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA). The membrane was washed thoroughly and then incubated with HRP-conjugated goat anti-rabbit IgG antibody (1:1,000, Santa Cruz Biotechnology, CA). Bands were visualized using an enhanced chemiluminescence detection system (Super Signal ECL kit; West Pico; Pierce, Rockford, IL, USA).

Tao Y., Yang Z., Fang W., Ma Z., Huang Y.F, & Li Z. (2017). Adeno-associated virus-mediated neuroglobin overexpression ameliorates the N-methyl-N-nitrosourea-induced retinal impairments: a novel therapeutic strategy against photoreceptor degeneration. Therapeutics and Clinical Risk Management, 13, 1379-1389.

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Detecting NY-ESO-1 Protein by Western Blot

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The final purified NY-ESO-1 proteins were detected by Western blot (WB) analysis. Briefly, the proteins were exposed to sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. After proteins were transferred to nitrocellulose membrane and subsequent blocking, the membranes were immunoblotted with rabbit anti-mice NY-ESO-1 antibody and visualized with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), followed by enhanced chemiluminescence (Pierce Biotechnology, Rockford, IL, USA) and auto-radiography.

Wang H., Huang W., Gao H, & Liu T.T. (2020). NY-ESO-1 Protein Vaccine Combining Alum, CpG ODN, and HH2 Complex Adjuvant Induces Protective and Therapeutic Anti-Tumor Responses in Murine Multiple Myeloma. OncoTargets and therapy, 13, 8069-8077.

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Apoptosis Evaluation in SCC4 Tumors

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Cleaved caspase-3 immunohistochemistry and terminal deoxyribonucleotide transferase (TdT)-mediated nick-end labeling (TUNEL) staining was performed to detect apoptotic cells in SCC4 tumor tissues of 5 study groups using paraffin-embedded sections of SCC4 tumor tissues. After fixation with cold acetone for 20 min, samples were incubated with a 1:300 dilution of rabbit polyclonal cleaved caspase-3 (Asp175) antibody (#9661, Cell signaling, Danvers, MA, US) at 4 °C for 1 h followed by incubation with HRP conjugated goat anti-rabbit IgG antibody (1:200, Santa Cruz Biotechnology, Santa Cruz, CA) for 30 min. Visualization of cleaved caspase-3 was achieved with a DAB detection kit (Pierce, Rockland, IL). TUNEL assay was performed in 5 study groups using an in situ cell death detection kit, POD, TUNEL System (Roche, Basel, Switzerland). This detection kit is based on the detection of single-and double-stranded DNA breaks that occur at the early stages of apoptosis. Cell nuclei with DAB substrate staining were defined as TUNEL-positive nuclei. TUNEL-positive nuclei were monitored. All specimens were examined with an Olympus BX53 light microscope (Olympus Corp., Tokyo, JP). Multiple (7 images) tumor images of cleaved caspase-3 and Tunel on high power (400×) of 5 groups were obtained using software image for analysis.

Chen W.H., Lecaros R.L., Tseng Y.C., Huang L, & Hsu Y.C. (2015). Nanoparticle delivery of HIF1α siRNA combined with photodynamic therapy as a potential treatment strategy for head-and-neck cancer. Cancer letters, 359(1), 65-74.

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Quantifying Mucus Protein Levels via ELISA

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To assess mucus production at the protein level, the levels of mucin 5AC (MUC5AC) and chloride channel accessory 1 (CLCA1) were determined in samples that were assayed in triplicate on 96-well white half-area flat-bottom ELISA plates (Greiner Bio-One North America) after incubation at 37 °C overnight. The assay for MUC5AC was performed using biotin-conjugated mouse anti-MUC5AC mAb (45M1, Invitrogen, #MA5-12175) as described previously (12 (link)). The assay for CLCA1 was performed using rabbit anti-human CLCA1 (amino acid 33–63) and HRP-conjugated goat anti-rabbit IgG antibody (Santa Cruz Biotechnology) as described previously (12 (link)). Assay reactions were developed using the GLO substrate kit (R&D Company) and quantified by comparison to a standard curve with recombinant proteins.

Keeler S.P., Wu K., Zhang Y., Mao D., Li M., Iberg C.A., Austin S.R., Glaser S.A., Yantis J., Podgorny S., Brody S.L., Chartock J.R., Han Z., Byers D.E., Romero A.G, & Holtzman M.J. (2023). A potent MAPK13–14 inhibitor prevents airway inflammation and mucus production. bioRxiv.

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Antibody Panel for Robo4 and Associated Proteins

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Anti-N-terminal mouse Robo4 antibody (Abcam, ab10547), anti-N-terminal human Robo4 antibody (R&D Systems, MAB2454), anti-FLAG antibody (Thermo Fisher Scientific, 14-6681-82), anti-HA antibody (Chromotek, #7c9-100), anti-intracellular Robo4 domain antibody (Santa Cruz Biotechnology, sc46497), anti-ADAM10-pro antibody (Abcam, ab39178), anti-ADAM10 antibody (Bioss, bs-3574R; LSbio, C497146-200; Novus, #NBP1-76973), anti-ADAM17 antibody (Bioss, 4236R; Novus #NBP2-61719), anti-Synecan-1 antibody (Santa Cruz Biotechnology, sc-5632), anti-actin antibody (Sigma Aldrich, A2228), anti-GAPDH antibody (R&D Systems, AF5718), polyclonal anti-Unc5B antibody (R&D Systems, AF1006), anti-N-terminal CDH5 (Thermo Fisher Scientific, 14-1441-82), anti-CD31 (BD, #550274), HRP-conjugated anti-His antibody (Alpha Diagnostic International, HISP12-HRP), HRP-conjugated goat anti-rabbit IgG antibody (Santa Cruz Biotechnology, sc-2030), HRP-conjugated donkey anti-goat IgG antibody (Santa Cruz Biotechnology, sc-2020), and HRP-conjugated goat anti-mouse IgG antibody (Invitrogen, 62-6520).

Xiao W., Pinilla-Baquero A., Faulkner J., Song X., Prabhakar P., Qiu H., Moremen K.W., Ludwig A., Dempsey P.J., Azadi P, & Wang L. (2022). Robo4 is constitutively shed by ADAMs from endothelial cells and the shed Robo4 functions to inhibit Slit3-induced angiogenesis. Scientific Reports, 12, 4352.

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Protein Extraction from Fungal Conidia

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For protein extraction, 10⁶ conidia were inoculated in 50 ml ACM and incubated at 25°C for 16 or 20 h. Mycelia were collected by filtering through Miracloth, snap-frozen in liquid nitrogen and lyophilised. The extraction of the proteins was performed as previously described [38 (link)]. The total protein concentration was determined using the commercially available bicinchoninic acid assay (BCA) assay (Sigma) according to manufacturer's instructions. Protein samples were separated on 15% SDS-PAGE gels. Recombinant aequorin was detected using a polyclonal rabbit anti-aequorin antibody (1:2000, Abcam) coupled with an HRP conjugated goat anti-rabbit IgG antibody (1:10000, Santa Cruz, USA).

Muñoz A., Bertuzzi M., Bettgenhaeuser J., Iakobachvili N., Bignell E.M, & Read N.D. (2015). Different Stress-Induced Calcium Signatures Are Reported by Aequorin-Mediated Calcium Measurements in Living Cells of Aspergillus fumigatus. PLoS ONE, 10(9), e0138008.

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