SH-SY5Y cells were obtained from ATCC, U.S.A (CRL-2266) and were thawed and cultured as per the supplier’s instructions in DMEM/F12 (1:1) cell media (Biowest, U.S.A), where DMEM stands for Dulbecco’s modified Eagle medium, and F12 for Ham’s F12 medium. This medium was supplemented with 10% fetal bovine serum (FBS; Biowest, U.S.A), 1% penicillin G/streptomycin (Biowest, U.S.A), and 1% non-essential amino acids (Biowest, U.S.A), hereby denoted as SH-SY5Y maintenance media. Cells were cultured in T75 flask and split 1:4 when confluence exceeded 80%. The passage number of the cells was never allowed to exceed 20 above the passage number at which the cells were supplied.
Penicillin g streptomycin
Manufactured by Biowest
Sourced in United States
Penicillin G/streptomycin is a commonly used antibiotic solution for cell culture applications. It provides broad-spectrum antimicrobial activity to prevent bacterial contamination in cell culture media and samples.
Automatically generated - may contain errors
Lab products found in correlation
Non essential amino acids (neaa), by Biowest (1 mentions)
Dulbecco modified eagle medium (dmem), by Cytiva (1 mentions)
Cellmaxin, by GenDEPOT (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
Cell culture dishes, by SPL Life Sciences (1 mentions)
Du145, by Korean Cell Line Bank (1 mentions)
2 protocols using penicillin g streptomycin
1
SH-SY5Y Cell Culture Protocol
2
Culturing and Passaging Prostate Cancer Cell Lines
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The human PCa cell lines, PC-3 and DU145, were purchased from the Korean Cell Line Bank (Seoul, Korea). Both cell lines were cultured using a medium (DMEM, HyClone Laboratories, Chicago, IL, USA) supplemented with 10% fetal bovine serum (FBS; RMBIO, Missoula, MT, USA), 1% penicillin G/streptomycin (Bio west, San Marcos, TX, USA), 1% HEPES (Gibco by Life Technologies, Gaithersburg, MD, USA) and 0.05% cell maxin (GenDEPOT, Katy, TX, USA) in cell culture dishes (SPL Life Science, Pocheon, Korea) at 37 °C in a humidified atmosphere containing 95% air and 5% CO2. Both cell lines were detached by using 0.05% Trypsin-EDTA (Gibco by Life Technologies, Gaithersburg, MD, USA).
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