Microsomal fractions were prepared as described previously [25 (link)] and stored at − 70°C until required. Microsomal protein concentrations were determined using the Bio-Rad Protein Assay Reagent (Bio-Rad Laboratories) and Western blot analysis was preformed as described previously [26 (link)] using polyclonal antisera to rat P450s and POR [27 (link),28 (link)]. Immunoreactive proteins were detected using horseradish peroxidase (HRP)-conjugated polyclonal goat anti-rabbit, anti-mouse or anti-sheep immunoglobulin secondary antibodies, as appropriate (Dako). Bands were visualized using Immobilon™ chemiluminescent HRP-conjugated substrate (Millipore) and a FUJIFILM LAS-3000 mini imaging system (Fujifilm UK.). Densitometric analysis was performed using Multi Gauge V2.2 software (Fujifilm UK).
Anti sheep immunoglobulin secondary antibodies
Manufactured by Agilent Technologies
Anti-sheep immunoglobulin secondary antibodies are laboratory reagents used to detect and quantify sheep-derived primary antibodies in various immunoassays and immunochemical applications. These secondary antibodies are labeled with enzymes, fluorescent dyes, or other reporter molecules to enable signal detection and amplification.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase hrp conjugated polyclonal goat anti rabbit, by Agilent Technologies (2 mentions)
Immobilon chemiluminescent hrp conjugated substrate, by Merck Group (2 mentions)
Multi gauge v2, by Fujifilm (2 mentions)
Las 3000 mini imaging system, by Fujifilm (2 mentions)
Protein assay reagent, by Bio-Rad (2 mentions)
2 protocols using anti sheep immunoglobulin secondary antibodies
1
Western Blotting of Microsomal P450s
2
Western Blotting of Microsomal P450s
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Microsomal fractions were prepared as described previously [25 (link)] and stored at − 70°C until required. Microsomal protein concentrations were determined using the Bio-Rad Protein Assay Reagent (Bio-Rad Laboratories) and Western blot analysis was preformed as described previously [26 (link)] using polyclonal antisera to rat P450s and POR [27 (link),28 (link)]. Immunoreactive proteins were detected using horseradish peroxidase (HRP)-conjugated polyclonal goat anti-rabbit, anti-mouse or anti-sheep immunoglobulin secondary antibodies, as appropriate (Dako). Bands were visualized using Immobilon™ chemiluminescent HRP-conjugated substrate (Millipore) and a FUJIFILM LAS-3000 mini imaging system (Fujifilm UK.). Densitometric analysis was performed using Multi Gauge V2.2 software (Fujifilm UK).
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