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Mouse ho 1 elisa set

Manufactured by Enzo Life Sciences
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The Mouse HO-1 ELISA set is a laboratory equipment product used to measure the concentration of Heme Oxygenase-1 (HO-1) in mouse samples. It provides a quantitative determination of HO-1 levels using the Enzyme-Linked Immunosorbent Assay (ELISA) technique.

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Lab products found in correlation

Complete ultra protease inhibitor cocktail, by Roche (2 mentions) Cell lysis buffer, by Cell Signaling Technology (2 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Merck Group (2 mentions) Reducing buffer, by Thermo Fisher Scientific (2 mentions) Mini protean tgx gel, by Bio-Rad (2 mentions) Precellys evolution, by Bertin Technologies (2 mentions)

2 protocols using mouse ho 1 elisa set

1

Quantifying Protein Expression in Mtb-Infected Lungs

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Perfused lungs from naïve and Mtb-infected mice were disrupted in 2 ml tubes containing 2.7 mm glass beads and PBS with cOmplete ULTRA™ protease inhibitor cocktail (Roche, Basel, Switzerland) and 2mM of PMSF (Sigma-Aldrich, Saint Louis, MO), using a Precellys Evolution™ tissue homogenizer (Bertin Instruments, Montigny-le-Bretonneux, France). Cell cultures were lysed in Cell Lysis Buffer (Cell Signaling Technology, Danvers, MA) containing cOmplete ULTRA™ protease inhibitor cocktail (Roche, Basel Switzerland) and 2mM of PMSF (Sigma-Aldrich, Saint Louis, MO). For western blot, samples were denatured for 5 min at 95°C in reducing buffer (ThermoFisher Scientific, Waltham, MA), separated in mini-protean TGX gels (Bio-Rad, Hercules, CA) and transferred to PVDF membranes prior to staining with specific antibodies and development with luminol. Protein expression in samples was calculated utilizing ImageJ software in relation to β-actin expression. The concentration of HO-1 in samples was measured by ELISA using mouse HO-1 ELISA set (Enzo Life Sciences, Farmingdale, NY). Antibody specifications are provided in Table S1.
Costa D.L., Amaral E.P., Namasivayam S., Mittereder L.R., Fisher L., Bonfim C.C., Sardinha-Silva A., Thompson R.W., Hieny S.E., Andrade B.B, & Sher A. (2020). Heme oxygenase-1 inhibition promotes IFNγ and NOS2 mediated control of Mycobacterium tuberculosis infection. Mucosal immunology, 14(1), 253-266.
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2

Quantifying Protein Expression in Mtb-Infected Lungs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Perfused lungs from naïve and Mtb-infected mice were disrupted in 2 ml tubes containing 2.7 mm glass beads and PBS with cOmplete ULTRA™ protease inhibitor cocktail (Roche, Basel, Switzerland) and 2mM of PMSF (Sigma-Aldrich, Saint Louis, MO), using a Precellys Evolution™ tissue homogenizer (Bertin Instruments, Montigny-le-Bretonneux, France). Cell cultures were lysed in Cell Lysis Buffer (Cell Signaling Technology, Danvers, MA) containing cOmplete ULTRA™ protease inhibitor cocktail (Roche, Basel Switzerland) and 2mM of PMSF (Sigma-Aldrich, Saint Louis, MO). For western blot, samples were denatured for 5 min at 95°C in reducing buffer (ThermoFisher Scientific, Waltham, MA), separated in mini-protean TGX gels (Bio-Rad, Hercules, CA) and transferred to PVDF membranes prior to staining with specific antibodies and development with luminol. Protein expression in samples was calculated utilizing ImageJ software in relation to β-actin expression. The concentration of HO-1 in samples was measured by ELISA using mouse HO-1 ELISA set (Enzo Life Sciences, Farmingdale, NY). Antibody specifications are provided in Table S1.
Costa D.L., Amaral E.P., Namasivayam S., Mittereder L.R., Fisher L., Bonfim C.C., Sardinha-Silva A., Thompson R.W., Hieny S.E., Andrade B.B, & Sher A. (2020). Heme oxygenase-1 inhibition promotes IFNγ and NOS2 mediated control of Mycobacterium tuberculosis infection. Mucosal immunology, 14(1), 253-266.
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