Fitc conjugated ebers oligonucleotides

EBERs in situ hybridization (ISH) was performed on FFPE tissue sections using fluorescein isothiocyanate (FITC)-conjugated EBERs oligonucleotides as probes (Dako, Carpinteria, CA, USA). A monoclonal antibody anti-FITC labeled with alkaline phosphatase was used for the detection of hybridized sites (Dako), with NBT-BCIP as a substrate for the enzyme, according to manufacturer’s instructions. The cases were visualized at a visible spectrum.

Epstein-Barr virus association was evaluated by EBERs in situ hybridization (EBER-ISH) and immunohistochemistry. EBER-ISH was performed on FFPE tissue sections using fluorescein isothiocyanate (FITC)-conjugated EBERs oligonucleotides as probes, following the manufacturer’s instructions (Dako). A monoclonal antibody anti-FITC labeled with alkaline phosphatase was used for the detection of EBERs probes (Dako), as described^{29 (link)}.
Immunostaining was used to localize viral LMP1 expression in tumor cells, using monoclonal antibodies CS1-4 (Dako). IHC detection of primary antibody was carried out using a universal streptavidin–biotin complex-peroxidase detection system (UltraTek HRP Anti- Polyvalent Lab Pack, ScyTek, Logan, Utah, USA) according to the manufacturer’s instructions^{29 (link)}.

EBERs in situ hybridization (ISH) was performed in formalin fixed paraffin-embedded (FFPE) tissue sections, using fluorescein isothiocyanate (FITC)-conjugated EBERs oligonucleotides as probes (Dako). A monoclonal antibody anti-FITC labeled with alkaline phosphatase was used to detect hybridized sites. In each hybridization run, an EBV-positive Hodgkin lymphoma FFPE paraffin-embedded tissue block was used as a control slide. A cutoff 20% of positive tumor cells we considered to define EBV+ DLBCL, NOS, was described [21 (link)].