Diethylstilbestrol des

Diethylstilbestrol (DES) and bisphenol A (BPA; 99% pure) were obtained from Sigma-Aldrich, St. Louis, MO, USA. Triton X-100 was also purchased from Sigma. Tocopherol-stripped corn oil was from MP Biomedicals (Santa Ana, CA, USA). Silastic tubing (o.d, 0.125 in x i.d. 0.062 in, #508-008) was purchased from Fisher Scientific (San Diego, CA, USA).

Diethylstilbestrol (DES, CAS no. 56-53-1; purity $\ge$ 99%) and Ketoconazole (KTZ, CAS no. 65277-42-1; purity 98%) were purchased from Sigma-Aldrich and BOC Sciences Inc., USA, respectively. Corn oil was purchased from Sigma-Aldrich (cat.no. C8267-2.5 L) and used as control and vehicle. Solutions used for dosing of animals were stored in glass bottles in the dark at room temperature and continuously stirred during the dosing period.

1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC), 1,2-dielaidoyl-sn-glycero-3-phosphoethanolamine (DEPE), and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) were produced by Avanti Polar Lipids (Alabaster, AL, USA) and purchased from Sigma-Aldrich (Madrid, Spain).
Diethylstilbestrol (DES) and all other reagents and solvents used in the experiments were acquired from Sigma-Aldrich (Madrid, Spain).

Recombinant human apo-lactoferrin was obtained from Sigma-Aldrich (St. Louis, MO, USA) and Ventria Bioscience (Aurora, CO, USA). Diethylstilbestrol (DES), nigericin, nystatin, piericidin A, tetraethylammonium (TEA), Tris(hydroxymethyl)amino-methane (Tris), and valinomycin were purchased from Sigma-Aldrich. All chemicals used were of analytical grade and were supplied by Sigma-Aldrich. Sabouraud-2% dextrose broth (SDB) was purchased from Merck KGaA (Darmstadt, Germany).

Surgical instruments including Castroviejo scissors, uniband LA-1 micro point scissors, serrated jaw MF-2 micro forceps, fully curved micro forceps MF-3, straight micro forceps soldering tweezers, insertion/extraction tweezers and anti-wicking tweezers were purchased from Cedarlane labs – Canada. Surgical sutures including 5-0 Perma Hand Silk Black 1X18’’ PS-3, 5-0 Perma Hand Silk Black 2X60’’ no needle and 4-0 Perma Hand Silk Black 1X18’’ G-3 were purchased from the Ethicon – USA. Diethylstilbestrol (DES) was purchased from Sigma – USA and prepared in mineral oil (Sigma-Aldrich, USA). GIMA 2mm diameter biopsy punch (GIMA, UK) was used to divide the endometrial layer into 2mm pieces. An 18G syringe needle (BD, USA) attached to a tuberculin syringe (BD, USA) was used to deliver endometrial specimens into the peritoneal cavity. Banamine (Merck, USA) solution was used every 12 hours for 2 two days to alleviate the pain during post-surgical care period. DMEM/F12 medium was used for preparation of endometrial transplant tissue (Sigma-Aldrich, USA). Skin incisions were closed with a Reflex Clip Applier (World Precision Instrument, USA) and clips were removed using Reflex Clip Removing Forceps (World Precision Instrument, USA).

Recombinant FSH and purified hCG were supplied by Dr. A. Parlow and the National Hormone and Peptide Program (National Institute of Diabetes and Digestive and Kidney Disease, National Institutes of Health, Torrance, CA, USA). Dulbecco modified Eagle medium (DMEM), DMEM/Ham's Nutrient mixture F-12, diethylstilbestrol (DES) and β-Estradiol-Water Soluble were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Gentamicin sulfate and Fungizone were purchased from Invitrogen Corp. (Carlsbad, CA, USA).
hCG for the in vivo study was provided by Schering-Plough Corp. (Osaka, Japan), and PMSG was provided by Asuka Pharmaceutical Co., Ltd. (Tokyo, Japan).