CMC, xyloglucan, lichenan, curdlan, laminarin, pustulan, glucomannan, and galactomannan were purchased from Megazyme International (Bray, Ireland). MCC was obtained from Funakoshi (Tokyo, Japan).
Laminarin
Manufactured by Megazyme
Sourced in Ireland
Laminarin is a water-soluble polysaccharide extracted from brown seaweed. It is used as a reference standard in various analytical and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Lichenan, by Megazyme (3 mentions)
Xyloglucan, by Megazyme (2 mentions)
Glucomannan, by Megazyme (1 mentions)
Curdlan, by Megazyme (1 mentions)
Galactomannan, by Megazyme (1 mentions)
Sodium carboxymethyl cellulose na cmc, by Merck Group (1 mentions)
Barley β glucan, by Megazyme (1 mentions)
Whatman filter paper, by GE Healthcare (1 mentions)
Carboxymethyl cellulose, by Maclin Biochemical Technology (1 mentions)
Oat spelt xylan, by Megazyme (1 mentions)
Bl21 de3 competent cells, by Vazyme (1 mentions)
Dpni, by New England Biolabs (1 mentions)
Primestar max dna polymerase, by Takara Bio (1 mentions)
T5 exonuclease, by New England Biolabs (1 mentions)
β glucan, by Megazyme (1 mentions)
Beechwood xylan, by Megazyme (1 mentions)
Wheat arabinoxylan, by Megazyme (1 mentions)
3 protocols using laminarin
1
Polysaccharide Characterization Protocol
2
Enzymatic Activity Assay for β-Glucans
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TlSWO activity was measured using the 3,5-dinitrosalicylic acid assay (Miller, 1959 (link)). Enzyme activity was assayed in a final volume of 1.5 mL, with 1% (w/v) barley β-glucan (Megazyme Co., Bray, Ireland), lichenan (Megazyme Co.), laminarin (Megazyme, Wicklow, Ireland) and carboxymethyl cellulose sodium (CMC-Na) (Sigma-Aldrich, St. Louis, MO, United States) as the substrates, and 10 μg/mL of enzyme at optimal conditions for 10 min. One unit of enzyme activity was defined as the amount of enzyme required to release 1 μmol of reducing sugars in 1 min.
3
Recombinant Enzyme Expression and Purification
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The E. coli DH5α and BL21 (DE3) competent cells were obtained from Vazyme (Nanjing, China). pET28a (+) vector from Novagen (Darmstadt, Germany) was used for protein expression. Prime STAR Max DNA polymerase, DNA and protein ladders were purchased from Takara (Osaka, Japan). The T5 Exonuclease and DpnI from NEB (Ipswich, MA, USA) were used for In-Fusion clone. The Ni2+ affinity resin (Qiagen, Germany) was utilized for protein purification. Carboxymethylcellulose (CMC), locust bean gum, Avicel and sugarcane xylan were obtained from Macklin (Shanghai, China). α and β-p-Nitrophenyl-β-D-glucopyranoside (α-pNPG and β-pNPG) were from Sigma–Aldrich (St. Louis, MO, USA). Beechwood xylan, oat spelt xylan, wheat arabinoxylan, β-glucan, lichenan, laminarin and xyloglucan were obtained from Megazyme (Bray, Ireland). Whatman filter paper was obtained from GE (Boston, USA).
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