XRD patterns were recorded on a Dan-dong Aolong/Y-2000 X-ray diffractometer (Dan Dong, China) with Cu Ka radiation (k = 0.15406 nm). The operating voltage was set at 40 kV, and the current was 40 mA. FT-IR spectra were recorded using a Shimadzu IRPrestige-21 Fourier transform spectrometer (Japan) by blending the sample into a KBr pellet. The measurement of specific surface area of samples was performed using nitrogen adsorption isotherm by specific surface area analyzer (ASAP2020M, Micromeritics Instrument Corp., USA). The UV-Vis light absorption spectra were obtained from a Hitachi UV-3010 spectrophotometer. The particle size and morphology were observed on a JEOL JEM-2010 (HR) transmission electron microscope.
Uv 3010 spectrophotometer
Manufactured by Hitachi
Sourced in Japan
The UV-3010 spectrophotometer is a laboratory instrument manufactured by Hitachi. It is designed to measure the absorbance or transmittance of samples across the ultraviolet and visible light spectrum. The core function of the UV-3010 is to provide accurate and reliable spectroscopic data for various applications in scientific research and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Jem 2010hr transmission electron microscope, by JEOL (1 mentions)
Asap 2020m, by Micromeritics (1 mentions)
F 4500 spectrofluorometer, by Hitachi (1 mentions)
Apex 2 30e spectrometer, by Bruker (1 mentions)
Ft ir prestige 21 spectrometer, by Shimadzu (1 mentions)
Sepa 300 digital polarimeter, by Horiba (1 mentions)
Amx 400, by Bruker (1 mentions)
Silica gel 60 f254, by Merck Group (1 mentions)
Avance 300, by Bruker (1 mentions)
Silica gel, by Merck Group (1 mentions)
Melting point apparatus, by Thermo Fisher Scientific (1 mentions)
Ft ir 410 spectrophotometer, by Jasco (1 mentions)
Avance 400 spectrometer, by Bruker (1 mentions)
Fls920 spectrofluorometer, by Edinburgh Instruments (1 mentions)
Ls 55 fluorescence spectrophotometer, by PerkinElmer (1 mentions)
2 n morpholino ethanesulfonic acid mes, by Merck Group (1 mentions)
Trypsin, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Na2hpo4, by Merck Group (1 mentions)
Tetrahydrofuran, by Merck Group (1 mentions)
7 protocols using uv 3010 spectrophotometer
1
Characterization of Nanomaterials Using XRD, FT-IR, and TEM
2
Flow-Injection Chemiluminescence Analysis System
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All CL measurements were performed on the IFFM-E mode flow-injection chemiluminescence (FI-CL) analysis system (Xi'an Remax Company, Xi'an, China). It has two peristaltic pumps and one injection system synchronized by a microprocessor. All the reactor coils were made of Teflon tubing. The flow cell was a glass tube (i.d. 0.5 mm) connected with a selected high sensitivity, and low-noise photomultiplier tube. Light measurement data (ICL) were transferred to a computer automatically. Data acquisition and treatment were used with REMAX software running under Windows XP. The photoluminescence spectra and UV-visible absorption spectra were performed on a model F-4500 spectrofluorometer (Hitachi, Tokyo, Japan) and a model UV-3010 spectrophotometer (Hitachi, Japan), respectively. The transmission electron microscopy (TEM) images of the nanoparticles were acquired on a JEM-2010 F microscope. The CL spectrum was detected and recorded by a BPCL-2-KIC Ultra-Weak Luminescence Analyzer (Institute of Biophysics, Chinese Academy of Sciences) and combined with a flow injection system.
3
Spectroscopic Analysis of Compound Characterization
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UV spectra were obtained with a Hitachi UV-3210 and UV-3010 spectrophotometer (Hitachi, Tokyo, Japan), and IR spectra were measured with a Shimadzu FTIR Prestige-21 spectrometer (Shimadzu, Kyoto, Japan). Optical rotations were measured with a HORIBA SEPA-300 digital polarimeter in a 0.5 dm cell (Horiba, Kyoto, Japan). The ESIMS and HRESIMS were taken on a Bruker Daltonics APEX II 30e spectrometer (Bruker, Billerica, MA, USA). 1H- and 13C-NMR spectra were measured using Bruker Avance-300, AMX-400, and AV-500 spectrometers (Bruker, Billerica, MA, USA) with TMS as the internal reference, and chemical shifts are expressed in δ (ppm). Silica gel (70–230 and 230–400 mesh; Merck, Darmstadt, Germany) and Spherical C18 100 Å reversed phase Silica gel (RP-18; particle size 20–40 μm; Silicycle, Quebec City, QC, Canada) were used for column chromatography (CC), and Silica gel 60 F254 and RP-18 F254S thin-layer chromatography (TLC) plates (Merck, Darmstadt, Germany) were used for preparative TLC, respectively.
4
Thermal and Spectroscopic Analysis
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Melting points were measured on a Fisher Scientific melting point apparatus and were uncorrected. UV spectra were recorded on a Hitachi UV-3010 spectrophotometer in MeOH solution. IR spectra were recorded on a Jasco FT-IR-410 spectrophotometer as KBr discs. The 1H- and 13C-NMR spectra were recorded on a Bruker Avance-400 spectrometer. Chemical shifts values are given with tetramethylsilane as an internal reference.
5
Characterization of Polymer Nanoparticles
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Fluorescence
measurements
were performed on an LS-55 fluorescence spectrophotometer equipped
with a 1.50 mL quartz cell (PerkinElmer, USA). UV–vis absorption
spectra were obtained with a UV-3010 spectrophotometer (Hitachi, Japan).
TEM images were acquired with a Hitachi HT-7700 transmission electron
microscope (Tokyo, Japan). Luminescence lifetime was carried out on
an FLS920 spectrofluorometer (Edinburgh Instruments, UK). Zeta potential
and DLS were measured by a ZS90 zetasizer (Malvern, UK).
PFBT
(MW 164 000, polydispersity 3.4) was purchased from ADSDyes, Inc. (Quebec, Canada). NaH2PO4, Na2HPO4, tetrahydrofuran
(THF, anhydrous, ≥99.9%, inhibitor-free), ALP, AA2P, AchE,
NADH, TyR, ADH, GLDH, COD, BSA, trypsin, 2-(N-morpholino)ethanesulfonic
acid (MES), and reduced NEM were purchased from Sigma-Aldrich and
used as received. Unless indicated otherwise, all the commonly employed
reagents in this study are of at least analytical purity and used
without further purification. Ultrapure water (18.25 MΩ·cm)
was used throughout the experiments.
measurements
were performed on an LS-55 fluorescence spectrophotometer equipped
with a 1.50 mL quartz cell (PerkinElmer, USA). UV–vis absorption
spectra were obtained with a UV-3010 spectrophotometer (Hitachi, Japan).
TEM images were acquired with a Hitachi HT-7700 transmission electron
microscope (Tokyo, Japan). Luminescence lifetime was carried out on
an FLS920 spectrofluorometer (Edinburgh Instruments, UK). Zeta potential
and DLS were measured by a ZS90 zetasizer (Malvern, UK).
PFBT
(MW 164 000, polydispersity 3.4) was purchased from ADSDyes, Inc. (Quebec, Canada). NaH2PO4, Na2HPO4, tetrahydrofuran
(THF, anhydrous, ≥99.9%, inhibitor-free), ALP, AA2P, AchE,
NADH, TyR, ADH, GLDH, COD, BSA, trypsin, 2-(N-morpholino)ethanesulfonic
acid (MES), and reduced NEM were purchased from Sigma-Aldrich and
used as received. Unless indicated otherwise, all the commonly employed
reagents in this study are of at least analytical purity and used
without further purification. Ultrapure water (18.25 MΩ·cm)
was used throughout the experiments.
6
Characterization of 5-FU@DHA-UIO-66
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The morphology of 5-FU@DHA-UIO-66 was observed under a scanning electron microscope (SEM, Zeiss Field Emission SEM Supra55VP, Oberkochen, Germany). Fourier transform infrared (FT-IR) spectroscopy was performed using a Spectrum100 FT-IR spectrometer (4000–400 cm−1). Powder X-ray diffraction (PXRD) data were collected with a DX-2700B diffractometer (Dandong Haoyuan Instrument, Dandong, Liaoning, China) in the range of 5–50° (2θ) at a scan rate of 5°/min. Further, thermogravimetric analysis (TGA) was conducted under nitrogen utilizing a TGA/SDTA 851e analyzer (Mettler-Toledo, Highstown, NJ, USA) with the temperature rising from ambient temperature to 800 °C at a heating rate of 10 °C/min.
5-FU@DHA-UIO-66 was also subjected to ultraviolet (UV)-VIS spectroscopy utilizing a UV-3010 spectrophotometer (Hitachi, Tokyo, Japan). The solid-state 13C MAS NMR spectrum was recorded utilizing a contact time of 3 milliseconds on a Bruker AM-400 (400 MHz) spectrometer equipped with a 5.0 mm chemical probe (Bruker Daltonik GmbH, Bremen, Germany). The nitrogen adsorption-desorption isotherm was measured with a Autosorb AS-6B/IQ2 apparatus (Quantachrome Instruments, Boynton Beach, FL, USA) under nitrogen (77 K). Moreover, the concentration of 5-FU was measured utilizing UV-VIS spectroscopy.
5-FU@DHA-UIO-66 was also subjected to ultraviolet (UV)-VIS spectroscopy utilizing a UV-3010 spectrophotometer (Hitachi, Tokyo, Japan). The solid-state 13C MAS NMR spectrum was recorded utilizing a contact time of 3 milliseconds on a Bruker AM-400 (400 MHz) spectrometer equipped with a 5.0 mm chemical probe (Bruker Daltonik GmbH, Bremen, Germany). The nitrogen adsorption-desorption isotherm was measured with a Autosorb AS-6B/IQ2 apparatus (Quantachrome Instruments, Boynton Beach, FL, USA) under nitrogen (77 K). Moreover, the concentration of 5-FU was measured utilizing UV-VIS spectroscopy.
7
5-FU Drug Release Kinetics from DHA-UIO-66-NH2
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The loading amount of 5-FU in DHA-UIO-66-NH2 was calculated based on the determination of the concentration of 5-FU in the solution and supernatant at different time points by UV-3010 spectrophotometer (Hitachi). The release rate of 5-FU in PBS (pH =7.4 or 5.5) was measured by dialysis. Briefly, 200 µg 5-FU@DHA-UIO-66-NH2 was dispersed in 20 mL of PBS and subjected to dialysis (37±1 °C, 110 rpm). At different time points during the dialysis, 1 mL of the sample was taken from the supernatant for UV detection of 5-FU content, and 1 mL of fresh PBS was simultaneously added to the original system.
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