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Truseq v2 lt sample prep kit

Manufactured by Illumina
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The TruSeq v2 LT Sample Prep Kit is a laboratory equipment product designed for library preparation in DNA sequencing workflows. It provides the necessary reagents and consumables to convert DNA samples into libraries compatible with Illumina sequencing platforms. The kit includes enzymes, adapters, and other essential components required for this sample preparation process.

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Lab products found in correlation

Rneasy plus mini kit, by Qiagen (2 mentions) Iq sybr green supermix, by Bio-Rad (1 mentions) Ovation rna seq system, by Tecan (1 mentions)

Spelling variants of this product

TruSeq kits (44 citations) TruSeq v2 kit (38 citations) TruSeq® RNA LT Sample Prep Kit v2 (31 citations) LT Sample Prep Kit (12 citations) Illumina sample prep kit (8 citations) TruSeq DNA LT Sample Prep Kit v2 (6 citations) V2 kits (4 citations) Prep Kit V2 (2 citations) Kit v2 (2 citations)

2 protocols using truseq v2 lt sample prep kit

1

RNA-Seq Library Preparation and Differential Expression Analysis

Cited 1 time
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Total RNA was isolated using RNeasy Plus Mini Kit (Qiagen) following manufacturer’s protocol. RNA-seq library was prepared using the Truseq v2 LT Sample Prep Kit (Illumina). The sequencing reads from all RNA-seq experiments were aligned to human reference genome hg18 by TopHat (Trapnell et al., 2009 ). Differential gene expression analyses were performed using DEseq (Anders and Huber, 2010 (link)). See also Supplemental Experimental Procedures.
Xu J., Shao Z., Li D., Xie H., Kim W., Huang J., Taylor J.E., Pinello L., Glass K., Jaffe J.D., Yuan G.C, & Orkin S.H. (2015). Developmental control of Polycomb subunit composition by GATA factors mediates a switch to non-canonical functions. Molecular cell, 57(2), 304-316.
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2

Transcriptome Profiling by RNA-seq

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Total RNA was isolated using RNeasy Plus Mini Kit (Qiagen) following manufacturer’s protocol. RNA-seq library was prepared using the Truseq v2 LT Sample Prep Kit (Illumina) or the Ovation RNA-seq system (NuGEN). Sequencing reads from all RNA-seq experiments were aligned to human (hg19) reference genome by TopHat v2.0.13 (Trapnell et al., 2009 ) with the parameters: --solexaquals --no-novel-juncs. Quantitative RT-PCR (qRT-PCR) was performed using the iQ SYBR Green Supermix (Bio-Rad). Primer sequences are listed in Table S2.
Liu X., Zhang Y., Chen Y., Li M., Zhou F., Li K., Cao H., Ni M., Liu Y., Gu Z., Dickerson K.E., Xie S., Hon G.C., Xuan Z., Zhang M.Q., Shao Z, & Xu J. (2017). In Situ Capture of Chromatin Interactions by Biotinylated dCas9. Cell, 170(5), 1028-1043.e19.
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