Non-labelled acyl-CoA and radiolabelled [14C]acyl-CoA were synthesized from unlabelled fatty acids (Sigma-Aldrich, St. Louis, MO, USA) and 14C-labelled fatty acids (Perkin-Elmer) according to a modified method described by Sánchez et al. [60 (link)]. Other substrates for enzymes assays: non-labelled sn-1-18:1-lysophosphatidylethanolamine, sn-1-18:1-lysophosphatidylcholine and lipid standards for thin-layer chromatography were purchased from Larodan Fine Chemicals and Avanti Polar Lipids, respectively.
Sn 1 18 1 lysophosphatidylcholine
Manufactured by Larodan
Sourced in Sweden
Sn-1-18:1-lysophosphatidylcholine is a lipid that is used as a research reagent in laboratory settings. It is a naturally occurring lipid species that can be derived from various biological sources.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sn 1 18 1 lysophosphatidylcholine
1
Synthesis of Radiolabeled Acyl-CoA
2
Preparation and Characterization of Radiolabeled Lipids
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[1-14 C]-labelled fatty acids were purchased from Perkin Elmer (Waltham, MA, USA), and non-radioactive fatty acids and sn-1-18:1-lysophosphatidylcholine were purchased from Larodan (Malmö, Sweden). Free CoA, bovine serum albumin (BSA) and heptadecanoic acid methyl ester (17:0-Me) were supplied by Sigma-Aldrich (St. Louis, MO, USA). The standards for thin-layer chromatography were obtained from Avanti Polar Lipids (Alabaster, AL, USA). The acyl-CoAs and [1-14 C]-labelled acyl-CoAs were prepared according to the modified method described by Sánchez et al. (1973) . The other biochemicals and solvents used for analysis were from Merck (Darmstadt, Germany) or Sigma-Aldrich.
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