V850 pro

Experiments that investigated the impacts of different light wavelengths on SPs growth were conducted with the 1637-2 cell lines. The SPs of the 1637-2 with shoots and roots on the germination medium were transferred to the growth medium (selected above). The growth medium was supplemented with 1/2 WPM liquid medium (30 mL), 20 g/L s sucrose, and 1/5 flask volume of culture substrate (perlite and vermiculite = 1:1, respectively). Each SP was transferred into a dedicated flask (Ø6 cm x 9 cm high). The plantlets were cultured for one-month at 25 ± 1℃. Each group was treated with 15 replicates and repeated three times. LEDs (Philips, Amsterdam, Netherlands, 100 µmol/m²/s) with different red and blue ratios were employed as the light sources (B indicates blue light (400–500 nm); 5R5B indicates red light: blue light = 5:5; 7R3B indicates red light: blue light = 7:3; 8R2B indicates red light: blue light = 8:2; R indicates red light (600–700 nm)). A cool white fluorescent lamp (Philips, Amsterdam, Netherlands, 100 µmol/m²/s) served as the CK. The root and shoot lengths (total lengths of the aboveground parts) were measured with a scale after rinsing. The rootstock ratio indicated the root length to shoot length (R/S) ratio. The root tips, volume, surface area and average diameter were measured using a root scanner (EPSON, V850PRO, China).

Throughput was defined as the number of worms filtered per minute of processing time. To estimate the number of worms that were loaded into the filtration apparatus per culture plate, a suspension of worms was obtained by washing each of 6 typical, mixed-stage culture plates with 2 mL M9 buffer. Worms were pelleted by settlement (5 min at -20 C) after which the volume of the suspension was adjusted to 5 mL. The pellet was resuspended by agitation. Resuspended worms were transferred to 11 foodless NGM plates (20 μL per plate). Worms were allowed to disperse for approximately 10 min, then counted in images of the plates taken on a flat-bed scanner (Epson V850 Pro, Los Alamitos, CA, USA). This procedure yielded an estimated worm density of 5.6 worms/μL in the 5 mL suspension, which is equivalent to 4.7 × 10³ worms/plate. Throughput estimates were based on a typical value of 10 plates per filtration run. Throughput was computed as N/T_i where N is the estimated number of worms on 10 plates (4.7 × 10⁴), and T_i is processing time of run i which began at the moment the contents of the first culture plate were loaded into the pre-filtration filter stack and ended when worms were recovered from the filtration stack. Throughput values in Table 2 are within-method mean processing time (n = 3 runs). Processing time T for bleach synchronization included culture time (48 hr.).