Primary tumor specimens of CRC patients enrolled in CIPN studies were used for immunofluorescent staining. We used anti-CD3 and anti-CD40L to stain T cells and CD40 ligand, respectively. Alexa Fluor 594-conjugated secondary antibody was used for CD3, and Alexa 488-conjugated secondary antibody was used for CD40L. Tissue was co-stained with Hoechst to detect the nucleus. Whole tissue was scanned automatically using a TissueFAXS PLUS microscope (TissueGnostics, Vienna, Austria). In each sample, the region of interest (ROI) was randomly selected from 5 fields of the tumor microenvironment. Each field was 1.2 × 1.2 mm2. The number of CD3+CD40L+ cells was quantitated automatically by TissueQuest analysis software (RRID: SCR_014822).
Tissuefaxs plus microscope
Manufactured by TissueGnostics
Sourced in Austria
The TissueFAXS PLUS microscope is a high-performance imaging system designed for comprehensive tissue analysis. It combines advanced microscopy capabilities with integrated image acquisition and analysis tools. The core function of the TissueFAXS PLUS is to provide researchers and clinicians with a robust and versatile platform for capturing, processing, and quantifying high-quality images of tissue samples.
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Lab products found in correlation
3 protocols using tissuefaxs plus microscope
1
Quantifying T Cell and CD40 Ligand in CRC
2
Automated Quantification of Stained Cells
3
In Situ Hybridization of Honey Bee miRNA
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The honey bee brains were prepared according to Olivier et al. (2008) , with the modification that each brain was fixed in 4 % paraformaldehyde (PFA, Sigma) at 4 ºC for 30 min, and dehydrated in ascending concentrations of ethanol, embedded in paraffin, then sectioned 10 µm from the frontal side. In situ hybridization was performed according to the kit instructions of BOSTER (# MK10197). The main steps were as follows: the endogenous enzymes in the brain sections were firstly inactivated with 3% H 2 O 2 ; then the sections were treated with pepsin diluted with 3% citric acid for 20 min at room temperature, and washed using PBS; each section was incubated with 20 µl hybrid liquid of ame-miR-279a probe (5'ttaatgagtgtggatctagtca3') overnight in 40ºC; the reactions were blocked and sample incubated with biotinylated anti-mouse digoxin. Colour development was carried out according to the instructions of DAB kit. Finally, sections were dehydrated through a graded series of methanol, soaked with xylene, mounted with neutral gum and examined with a TissueFAXS plus microscope (TissueGnostics, Austria).
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