Mab dm1a

Manufactured by Merck Group

MAb DM1A is a laboratory product developed by Merck Group. It is a monoclonal antibody that can be used for research purposes. The core function of MAb DM1A is to serve as a tool for scientific investigations, though its specific applications may vary depending on the research context.

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Lab products found in correlation

Hybond n membrane, by Cytiva (2 mentions) Hybond, by Cytiva (2 mentions) Monoclonal anti flag m2 peroxidase antibody, by Merck Group (1 mentions)

Spelling variants of this product

Anti-α-tubulin mouse mAb (DM1A) (3 citations)

2 protocols using mab dm1a

Western Blot and Northern Analysis

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Western blot signals of FLAG, tubulin, and SmD2 were detected using Monoclonal anti-FLAG M2-Peroxidase antibody (Sigma), mAb DM1A (Sigma) and anti-SNRPD2 antibody (Abcam), respectively. Northern analyses were performed by transferring RNAs from 8 M Urea-PAGE gel to Hybond-N membrane (Amersham) and probed by ³²P-labeled antisense DNA oligonucleotides that are listed in Supplementary Data 5.

Li L., Ding Z., Pang T.L., Zhang B., Li C.H., Liang A.M., Wang Y.R., Zhou Y., Fan Y.J, & Xu Y.Z. (2020). Defective minor spliceosomes induce SMA-associated phenotypes through sensitive intron-containing neural genes in Drosophila. Nature Communications, 11, 5608.

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Drosophila U1-70K Immunoassay Protocol

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Antiserum against Drosophila U1-70K was generated by immunizing rabbits with the peptide DGKKIDSKRVLVDV (amino acids 164–177). Anti-SNRPD2 (SmD2) antibody was purchased from Abcam (ab155030). Western blots of anti-EGFP and anti-tubulin were probed using mAb 7G9 (Abmart) and mAb DM1A (Sigma), respectively. Northern analysis was performed by transferring RNAs from 8 M urea-PAGE gel to Hybond N membrane (Amersham). Membranes were then probed by DIG-labeled (Roche) antisense DNAs complementary to the full-length U snRNAs in D. melanogaster.

Gao J.L., Fan Y.J., Wang X.Y., Zhang Y., Pu J., Li L., Shao W., Zhan S., Hao J, & Xu Y.Z. (2015). A conserved intronic U1 snRNP-binding sequence promotes trans-splicing in Drosophila. Genes & Development, 29(7), 760-771.

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