ACS grade acetone was from VWR International S.A.S. (Fontenay-sous-Bois, France). LC–MS grade acetonitrile and formic acid was from Fluka Analytical (Sigma Aldrich Chemie GmbH, Steinheim, Germany). Water was purified with Millipore Synergy UV (Merck KGaA, Darmstadt, Germany) water purification system.
Millipore synergy uv
Manufactured by Merck Group
Sourced in Germany
The Millipore Synergy UV is a high-performance water purification system designed to produce ultrapure water for laboratory applications. It utilizes a combination of reverse osmosis and ultraviolet (UV) technologies to remove impurities and ensure consistent water quality. The system's core function is to provide a reliable and efficient source of ultrapure water for various laboratory processes and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Acs grade acetone, by Avantor (2 mentions)
Lc ms grade acetonitrile, by Merck Group (2 mentions)
Analytical grade acetone, by Avantor (2 mentions)
Formic acid, by Avantor (2 mentions)
Formic acid, by Merck Group (1 mentions)
Lc ms grade acetonitrile, by Avantor (1 mentions)
Lc ms grade formic acid, by Merck Group (1 mentions)
Acetone, by Avantor (1 mentions)
Acetonitrile, by Avantor (1 mentions)
Methanol, by Avantor (1 mentions)
Bovine serum albumin cohn fraction 5, by Merck Group (1 mentions)
Lc ms grade, by Merck Group (1 mentions)
5 protocols using millipore synergy uv
1
Purification of Chemical Solvents
2
UHPLC–MS Catechins Analysis
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Water used for UHPLC–MS
and acid butanol analyses was purified using a Millipore Synergy UV
(Merck KGaA, Darmstadt, Germany) water purification system. ACS-grade
acetone was obtained from VWR International S.A.S. (Fontenay-sous-Bois,
France) and LC–MS-grade acetonitrile and formic acid from Fluka
Analytical (Sigma-Aldrich Chemie GmbH, Steinheim, Germany). Catechin,
epicatechin, gallocatechin, and epigallocatechin were purchased from
Extrasynthese (Genay, France).
and acid butanol analyses was purified using a Millipore Synergy UV
(Merck KGaA, Darmstadt, Germany) water purification system. ACS-grade
acetone was obtained from VWR International S.A.S. (Fontenay-sous-Bois,
France) and LC–MS-grade acetonitrile and formic acid from Fluka
Analytical (Sigma-Aldrich Chemie GmbH, Steinheim, Germany). Catechin,
epicatechin, gallocatechin, and epigallocatechin were purchased from
Extrasynthese (Genay, France).
3
Analytical Extraction and UHPLC Analysis
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Analytical-grade acetone (VWR International S.A.S., France) was used during extraction.
For oxidation, a pH 10 carbonate buffer (50 mM; sodium carbonate/sodium hydrogen
carbonate, J.T. Baker, Deventer, Netherlands) and, for stopping the oxidation, formic acid
(J.T. Baker, Deventer, Netherlands) were used. For the UHPLC analysis, LC–MS-grade
acetonitrile was purchased from VWR International S.A.S. (USA), and LC–MS-grade
formic acid was purchased from Sigma Aldrich (Seelze, Germany). The Milli-Q water used was
purified with the Millipore Synergy UV (Merck KGaA, Darmstadt, Germany) system.
For oxidation, a pH 10 carbonate buffer (50 mM; sodium carbonate/sodium hydrogen
carbonate, J.T. Baker, Deventer, Netherlands) and, for stopping the oxidation, formic acid
(J.T. Baker, Deventer, Netherlands) were used. For the UHPLC analysis, LC–MS-grade
acetonitrile was purchased from VWR International S.A.S. (USA), and LC–MS-grade
formic acid was purchased from Sigma Aldrich (Seelze, Germany). The Milli-Q water used was
purified with the Millipore Synergy UV (Merck KGaA, Darmstadt, Germany) system.
4
Purification and Analysis of Biochemical Compounds
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The following chemicals were used in this study: acetone (extraction and Sephadex LH-20 fractionation: Analytical grade, VWR International S.A.S., Fontenay-sous-Bois, France), acetonitrile (semi-preparative purification: HPLC grade, VWR International S.A.S., EC; UPLC analyses: LC-MS grade, VWR International S.A.S., Radnor, PA, USA), methanol (analytical grade, VWR International S.A.S., Fontenay-sous-Bois, France), formic acid (semi-preparative purification: 99‒100%, VWR Chemicals, EC; UPLC analyses: LC-MS grade, Sigma Aldrich, St. Louis, MO, USA), Bovine Serum Albumin (BSA, Cohn Fraction V, lyophilized powder, purity ≥ 95%, Sigma Aldrich, St. Louis, MO, USA). Water was purified with Millipore Synergy UV (Merck KGaA, Darmstadt, Germany) system.
5
Liquid Chromatography-Mass Spectrometry Reagents
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LC-MS grade acetonitrile was from Sigma-Aldrich GmbH (Steinheim, Germany) and formic acid (for LC-MS) and analytical grade acetone from VWR (Helsinki, Finland). LC grade acetonitrile was from Lab-Scan (Dublin, Ireland) and phosphoric acid from J.T. Baker (Deventer, Netherlands). Water was purified with either a Millipore Synergy UV (Merck KGaA, Darmstadt, Germany) or an Elgastat UHQ-PS (Elga, Kaarst, Germany) water purification system. Acetone-d6 (99.96 %) was from Euriso-top SAS (St-Aubin Cedex, France).
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