The xCELLigence DP device (Roche Diagnostics, Germany) was used to monitor cell proliferation in real-time. The 96 well electronic microtiter plates (Roche Diagnostics, Germany) were used to culture H292 cells (5 × 105), and the cells were treated with various concentrations of 0404 or Nutlin or 5-FU, respectively. The xCELLigence system was used to measure all cells for 96 h according to the instructions. A programmed signal detection was used to measure the cell density in quadruplicate every 30 min. The RTCA software (version 1.2) from Roche Diagnostics was used for collecting and analyzing data.
96 well electronic microtiter plates
Manufactured by Roche
Sourced in Germany
The 96 well electronic microtiter plates are a laboratory equipment designed for high-throughput experiments. These plates contain 96 individual wells for holding samples or reagents. The plates are designed to be used with specialized plate readers and automated liquid handling systems to facilitate efficient and accurate data collection and analysis.
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Lab products found in correlation
2 protocols using 96 well electronic microtiter plates
1
Real-Time Proliferation Monitoring
2
Real-Time Cell Proliferation Assay
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An xCELLigence DP device (Roche Diagnostics, Germany) was used to monitor cell proliferation in real time. HCC cells (5 × 105) were seeded in 96-well electronic microtiter plates (Roche Diagnostics, Germany) and then were treated with Dox combined with AMSC-Exo-199a or AMSC-Exo-67. The xCELLigence system was used to measure all cells for 96 h according to the instructions. A programmed signal detector was used to measure the cell density in quadruplicate every 30 min. RTCA software (version 1.2) from Roche Diagnostics was used for collecting and analyzing data. For the 50% inhibitory concentration (IC50) assay, data were analyzed by GraphPad Prism 5 software (GraphPad, La Jolla, CA).
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