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Monoclonal phospho akt ser473

Manufactured by Cell Signaling Technology
Sourced in United States

Monoclonal phospho-Akt (Ser473) is a lab equipment product from Cell Signaling Technology. It is a monoclonal antibody that specifically recognizes the phosphorylated form of Akt at serine 473.

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2 protocols using monoclonal phospho akt ser473

1

Protein Expression Analysis in Rabbit

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Rabbit phospho-GSK-3β antibodies and monoclonal phospho-Akt (Ser473) were purchased from the Cell Signaling Technologies, Inc. (Beverly, MA, USA; cat. nos. 5558 and 4060, respectively). Rabbit monoclonal CTHRC1, C-myc and Ror2 antibodies (tyrosine-protein kinase transmembrane receptor Ror2) were purchased from Abcam, Inc. (Cambridge, MA, USA; cat. nos. ab85739, ab32072 and ab92379, respectively). Rabbit polyclonal c-Jun and Wnt5a antibodies were obtained from WanleiBio (Shenyang, China; cat. nos. wl0219a and wl0198, respectively). Goat anti-rabbit HRP-conjugated secondary antibodies and goat monoclonal HRP-conjugated antibody against GAPDH were obtained from Dingguo Biological Technology (Beijing, China; cat. nos. IH-0011 and SH-0031, respectively).
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2

Immunodetection of Signaling Proteins

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Polyclonal anti-SDF-1, monoclonal antibodies against phospho-JNK (Y185/Y223), JNK, and  IKK α + β, and polyclonal phospho-IKK α + β (S180/S181) antibodies were from Abcam PLC (Cambridge, UK). Polyclonal actin and polyclonal Akt antibodies were from Santa Cruz Biotechnology (Dallas, TX). Monoclonal phospho-Akt (Ser 473) and polyclonal IkB-α antibodies were from Cell Signaling Technology, Inc. (Beverly, MA). Monoclonal anti-vinculin antibodies were kindly provided by the lab of Prof. Geiger (Weizmann Institute of Science, Rehovot, Israel). Peroxidase-conjugated affinity purified goat anti-mouse, goat anti-rabbit and donkey anti-goat antibodies were all from Jackson ImmunoResearch Laboratories (West Grove, PA).
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